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排序方式: 共有68条查询结果,搜索用时 15 毫秒
1.
Takashi Naito Minoru Tanaka Satoshi Taba Tesuya Toyosato Atsushi Oshiro Kazuko Takaesu Kazuo Hokama Tomio Usugi Shinji Kawano 《Journal of General Plant Pathology》2007,73(2):139-141
In 1999, a disease of chrysanthemum [Dendranthema grandiflorum (Ramat.) Kitamura], characterized by virescence of flowers, occurred in Okinawa Prefecture. The causal agent was identified
as “Candidatus Phytoplasma aurantifolia” based on 16S rDNA sequencing.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB247462. 相似文献
2.
Takaaki Fujimoto Kazuhito Kita Kazuko Uchiyama Makoto Kuromaru Hisashi Akutsu Kazuyuki Oda 《Journal of Forest Research》2006,11(3):157-163
Age trends in variance components and heritability of overall wood density, earlywood and latewood density, and latewood proportion
were investigated in 29-year-old trees of 19 full-sib families of hybrid larch (Larix gmelinii var. japonica × Larix kaempferi) F1. The age–age correlation and optimum selection age for these traits were also estimated and genetic and phenotypic correlations
between wood density and radial growth rate were calculated for each growth ring. Intraring wood density data were obtained
using X-ray densitometry. The coefficient of additive genetic variance was stable over all ages, whereas the coefficient of
environmental variances gradually decreased with increasing age, resulting in increases in heritability estimates with age
for overall density. The latewood proportion had the highest heritability estimates at all ages, ranging from 0.44 to 0.66.
Overall density and its various components at 28 years of age showed strong genetic correlations with their respective traits
at all younger ages. Optimum selection ages for the wood density traits ranged from 8 to 14 years, at which point maximum
gain efficiencies per year were obtained. There were negative correlations between wood density and radial growth rate at
early ages, although these relationships tended to be weaker with increasing age. These results suggest that selection at
a young age is effective for wood density, but particular care must be taken in selecting trees with an improved radial growth
rate because rapid growth will result in a low-density wood product, especially in the early growth period. 相似文献
3.
Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers 总被引:1,自引:0,他引:1
A high‐throughput single nucleotide polymorphism (SNP) genotyping procedure was developed to select amylose‐free barley mutants whose waxy genes had a C‐ to T‐base substitution in exon 5, which converted Gln‐89 of the wild‐type gene into a termination codon. An F2 population carrying an amylose‐free waxy gene was checked for segregation. Polymerase chain reaction with confronting two‐pair primers (PCR‐CTPP) produced allele‐specific PCR products that have different sizes and are inherited in a co‐dominant manner. Two alleles of the barley waxy gene with SNP were correctly identified in parental strains using the PCR‐CTPP procedure. Segregation of the SNP as detected by PCR‐CTPP in an F2 population fitted the expected 1:2:1 ratio. The PCR‐CTPP procedure can provide a time saving and cost‐effective alternative to derived cleaved amplified polymorphic sequence in marker‐assisted selection. 相似文献
4.
Rensing SA Lang D Zimmer AD Terry A Salamov A Shapiro H Nishiyama T Perroud PF Lindquist EA Kamisugi Y Tanahashi T Sakakibara K Fujita T Oishi K Shin-I T Kuroki Y Toyoda A Suzuki Y Hashimoto S Yamaguchi K Sugano S Kohara Y Fujiyama A Anterola A Aoki S Ashton N Barbazuk WB Barker E Bennetzen JL Blankenship R Cho SH Dutcher SK Estelle M Fawcett JA Gundlach H Hanada K Heyl A Hicks KA Hughes J Lohr M Mayer K Melkozernov A Murata T Nelson DR Pils B Prigge M Reiss B Renner T Rombauts S Rushton PJ 《Science (New York, N.Y.)》2008,319(5859):64-69
We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics. 相似文献
5.
Pang PT Teng HK Zaitsev E Woo NT Sakata K Zhen S Teng KK Yung WH Hempstead BL Lu B 《Science (New York, N.Y.)》2004,306(5695):487-491
Long-term memory is thought to be mediated by protein synthesis-dependent, late-phase long-term potentiation (L-LTP). Two secretory proteins, tissue plasminogen activator (tPA) and brain-derived neurotrophic factor (BDNF), have been implicated in this process, but their relationship is unclear. Here we report that tPA, by activating the extracellular protease plasmin, converts the precursor proBDNF to the mature BDNF (mBDNF), and that such conversion is critical for L-LTP expression in mouse hippocampus. Moreover, application of mBDNF is sufficient to rescue L-LTP when protein synthesis is inhibited, which suggests that mBDNF is a key protein synthesis product for L-LTP expression. 相似文献
6.
The SAX-3/roundabout (Robo) receptor has SLT-1/Slit-dependent and -independent functions in guiding cell and axon migrations. We identified enhancer of ventral-axon guidance defects of unc-40 mutants (EVA-1) as a Caenorhabditis elegans transmembrane receptor for SLT-1. EVA-1 has two predicted galactose-binding ectodomains, acts cell-autonomously for SLT-1/Slit-dependent axon migration functions of SAX-3/Robo, binds to SLT-1 and SAX-3, colocalizes with SAX-3 on cells, and provides cell specificity to the activation of SAX-3 signaling by SLT-1. Double mutants of eva-1 or slt-1 with sax-3 mutations suggest that SAX-3 can (when slt-1 or eva-1 function is reduced) inhibit a parallel-acting guidance mechanism, which involves UNC-40/deleted in colorectal cancer. 相似文献
7.
8.
This study aimed at capturing the spatial variability of landscape patterns and their trajectories of change from 1950 to
2000 within a watershed, which is representative of areas of intensive agricultural use. After an analysis of landscape features
changes for the entire watershed based on aerial photographs, hierarchical clustering analysis provided a typology of landscape
patterns for the cadastral lots. Following that, the trajectory of change of each lot was characterized (nature, importance,
direction, rate of change). Seven types of landscape patterns are distinguished by the relative importance of different classes
of landscape features and 51 trajectories of change were identified for the lots. The analysis shows that although the majority
of lots were subjected to a homogenization of their landscape patterns since 1950, this trend is not entirely uniform and
that since 2000 it occurs alongside trends towards diversification of certain landscape features on some lots. Furthermore,
nearly a third of the lots are not following the main trajectories of change detected. Thus, the results suggest that extrinsic
forces (policies, technologies) that are directing main changes in areas of intensive agricultural use toward uniformity could
be modulated by internal forces (uses and values of the population). A better understanding of theses internal forces seems
crucial to manage landscapes. From a methodology standpoint, although the hierarchical clustering analyses appear useful for
understanding the spatial and temporal variability of landscape patterns, particular attention must be given to validating
the typology chosen to characterize them. 相似文献
9.
10.
Yonetomi D Kadosawa T Miyoshi K Nakao Y Homma E Hanazono K Yamada E Nakamura K Ijiri A Minegishi N Maetani S Hirayama K Taniyama H Nakade T 《Veterinary radiology & ultrasound》2012,53(4):371-380
Contrast‐enhanced magnetic resonance (MR) imaging with a new liver‐specific contrast agent gadolinium‐ethoxybenzyl‐diethylenetriamine penta‐acetic acid (Gd‐EOB‐DTPA; EOB·Primovist®) was studied in 14 normal beagles and 9 dogs with focal liver lesions. Gd‐EOB‐DTPA accumulates in normally functioning hepatocytes 20 min after injection. As with Gd‐DTPA, it is also possible to perform a dynamic multiphasic examination of the liver with Gd‐EOB‐DTPA, including an arterial phase and a portal venous phase. First, a reliable protocol was developed and the appropriate timings for the dynamic study and the parenchymal phase in normal dogs using Gd‐EOB‐DTPA were determined. Second, the patterns of these images were evaluated in patient dogs with hepatic masses. The optimal time of arterial imaging was from 15 s after injection, and the optimal time for portal venous imaging was from 40 s after injection. Meanwhile, the optimal time to observe changes during the hepatobiliary phase was from 20 min after injection. In patient dogs, 11 lesions were diagnosed as malignant tumors; all were hypointense to the surrounding normal liver parenchyma during the hepatobiliary phase. Even with a low‐field MR imaging unit, the sequences afforded images adequate to visualize the liver parenchyma and to detect tumors within an appropriate scan time. Contrast‐enhanced MR imaging with Gd‐EOB‐DTPA provides good demarcation on low‐field MR imaging for diagnosing canine focal liver lesions. 相似文献