The effect of protein binding on ivermectin uptake by bovine brain microvessel endothelial cells     

K. L. Audus  S. R. Knaub  F. L. Guillot  J. M. Schaeffer 《Veterinary research communications》1992,16(5):365-377

The effect of albumin binding on ivermectin uptake and transfer across the endothelial component of the blood-brain barrier (BBB) was determined with anin vitro model comprised of bovine brain microvessel endothelial cell (BMEC) monolayers. Cellular uptake of ivermectin was limited in the absence of albumin and 90% inhibited in the presence of 10% albumin. Cell membrane association of ivermectin, as followed by fluorescent probe labelling, was observed only at high (micromolar) concentrations of the drug. Membrane association was about 75% inhibited in the presence of albumin. Similarly, transfer across BMEC monolayers was restricted, equivalent to that of BBB impermeant markers. Unlike the uptake studies, however, albumin had little effect on the transfer of ivermectin across BMEC monolayers. These results support recentin vivo findings on the distribution of ivermectin into the brain and suggest that ivermectin has only a limited affinity for the endothelial component of the normal BBB.  相似文献   

Condition Factors and Length-Weight Relationships of Pond-Cultured Paddlefish Polyodon spathula with Reference to Other Morphogenetic Relationships     

Steven D.  Mims Richard S.  Knaub 《Journal of the World Aquaculture Society》1993,24(3):429-433

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