Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels          下载免费PDF全文

M Hajiahmadian  K Sarvi Moghanlou  N Agh  F Farrokhi Ardabili 《Reproduction in domestic animals》2016,51(6):979-984

Brood fish nutrition is an important factor susceptible to affect not only fecundity and gametogenesis but also gamete quality. In this study, we investigated the effects of altering dietary vegetable fatty acid content on semen quality (i.e. motility, density and seminal plasma composition), fertilizing ability and also blood testosterone (T) concentration in rainbow trout (Oncorhynchus mykiss). Fish were fed a commercial diet and ten formulated diets with similar proximate compositions but different levels of vegetable fatty acids (highly unsaturated fatty acids (HUFA): monounsaturated fatty acids (MUFA); HUFA: polyunsaturated fatty acids (PUFA); and HUFA: saturated fatty acids (SFA) ratios). Fish fed with HUFA: MUFA = 0.0 and HUFA: SFA = 0.25 ratios had the highest semen motility percentage and duration. However, the highest semen concentration and semenatocrit were observed in HUFA: SFA = 0.0 and HUFA: PUFA = 0.37 ratios. There was a significant difference in terms of K⁺ ion among diets supplemented with HUFA: PUFA = 0.0, HUFA: PUFA = 0.37 and HUFA: MUFA = 0.16 ratios (p < .05). Furthermore, Na⁺ ion showed significant difference between control group and diet supplemented with HUFA: PUFA = 0.0 (p < .05). Among the biochemical parameters, total protein showed a significant difference between HUFA: MUFA = 0.16 and HUFA: PUFA = 0.37 ratios (p < .05). No significant differences in fertilization ability and blood T concentration were found among dietary treatments (p > .05). In addition, the present data suggest that dietary fatty acid levels could affect semen quality but not fertilization ability in O. mykiss.  相似文献   

Anaesthetic efficacy of eugenol on Flowerhorn (Amphilophus labiatus × Amphilophus trimaculatus)          下载免费PDF全文

Reza Tarkhani  Ahmad Imani  Hadi Jamali  Kourosh Sarvi Moghanlou 《Aquaculture Research》2017,48(6):3207-3215

Anaesthetic efficacy of eugenol was investigated on Flowerhorn (Amphilophus labiatus × Amphilophus trimaculatus). A total of 104 fish with average weights of 12 ± 2.5, 28 ± 5 and 53 ±5.1 g were subjected to 25–200 mg L^?1 eugenol and behavioural responses as well as induction and recovery times were recorded. Induction and recovery times were significantly affected by eugenol concentration as well as fish weight (P < 0.05). Generally, 49.9–127.3 s after exposure to 50–200 mg L^?1 eugenol, fish reached stage 3 anaesthesia (suitable for general handling). Fish entered stage 4 anaesthesia (suitable for surgery and blood sampling) over 57.3–140.4 s post exposure to such concentrations. Recovery time was 91.7–312 s in all weight classes for all eugenol concentrations. Mortality (23%) was only observed in 12‐g fish when were subjected to 200 mg L^?1 eugenol. This study showed the behavioural response of Flowerhorn to anaesthesia and eugenol efficacy as an anaesthetic in this important ornamental species. The general quadratic equation revealed that concentrations of eugenol and fish size along with their interactive effects have significantly contributed to the model, with concentration recording the highest beta value in all models (β = ?0.809, ?0.818 and ?0.909, P = 0.000). According to the results, minimum eugenol concentration to induce anaesthesia in less than 3 min was 50 mg L^?1.  相似文献   

Short-Term Storage of Semen of Rainbow Trout: Interactions of Time,Antibiotic, and Activator     

Hamid Niksirat  Koorosh Sarvi  Asghar Abdoli  Saeed Hajirezaee 《Journal Of Applied Aquaculture》2013,25(4):358-366

This study was designed to examine the main effects and interactions of time, presence of antibiotics, and type of sperm activators on the fertilization capacity (eyeing rate) of refrigerated semen of rainbow trout, Oncorhynchus mykiss. The semen samples were stored in the presence or absence of 250 IU ml^?1 penicillin and 250 μg ml^?1 streptomycin sulfate. Freshwater and a saline solution were used as sperm activators. The semen samples were stored at 2–3°C and fertilized after 0, 6, 8, 12, 19, and 25 days of storage. Fertilizing capacities of semen samples stored in the presence of antibiotics (63.8 ± 5.6%) were significantly (p < 0.05) higher than those stored in the absence of antibiotics (46.2 ± 6.7%). Also, the fertilizing capacities of stored semen samples activated using saline solution (70.7 ± 5.7%) had significantly (p < 0.05) higher values than those activated using freshwater (39.3 ± 5.9%). Semen samples stored in the absence of antibiotics completely lost fertilizing capacity within 19 days of storage. After 25 days of storage in the presence of antibiotics, induction of fertilization using freshwater and saline solution resulted in 0% and 79.8 ± 1.7% fertility, respectively.  相似文献   

Replacing rotifers with a microparticle diet from first feeding in yellowfin seabream,Acanthopagrus latus (Houttuyn), larvae     

Behzad Sarvi  Abbas Matinfar  Homayoun Mahmoudzadeh  Gholam Reza Eskandary 《Aquaculture Research》2010,41(11):1614-1621

The possibility to replace or reduce rotifer use with a microparticle diet (MPD) from first feeding in yellowfin seabream larvae was investigated. The experiment consisted of five treatments, a rotifer (100%) control treatment (100R), three experimental treatments, which received a fixed ration of MPD supplemented with 75%, 50% and 25% of the amount rotifer fed in the control treatment (treatments 75R‐MPD, 50R‐MPD and 25R‐MPD respectively), and finally a treatment with the fixed MPD ration only (treatment 0R‐MPD). The results indicated that feeding regimes 100R, 75R‐MPD and 50R‐MPD did not differ significantly in the total length, final survival and stress test resistance. The highest dry weight was achieved in treatments 100R and 75R‐MPD at the end of the experiment compared with treatments 50R‐MPD, 25R‐MPD and 0R‐MPD. The 0R‐MPD treatment showed the lowest growth and survival. Yellowfin seabream larvae were able to ingest inert food directly from the moment of first feeding.  相似文献