The effect of combined Azospirillum inoculation and nitrogen fertilizer on plant growth promotion and yield response of the blanket flower Gaillardia pulchella     

Ravi S. Gadagi  P. U. Krishnaraj  J. H. Kulkarni  Tongmin Sa 《Scientia Horticulturae》2004,100(1-4):323-332

The investigation was carried out to examine the performance of Azospirillum isolates on growth and N uptake of Gaillardia pulchella with two nitrogen levels. Azospirillum strain OAD-2 inoculation significantly increased plant height, number of leaves per plant, branches per plant and total dry mass accumulation in G. pulchella than other inoculations and/or uninoculated control. The application of 150 kg N ha⁻¹ further enhanced the above plant growth parameters significantly over application of 112 kg N ha⁻¹. The N uptake of G. pulchella was enhanced due to Azospirillum strain OAD-2 inoculation, which was further augmented in the presence of nitrogen. The combination of Azospirillum strain OAD-2 and 150 kg N ha⁻¹ showed the highest N uptake at 120 DAT. Plant growth and yield parameters also increased when inoculated with the non-diazotrophic, but efficient IAA producing, Azospirillum strain OAD-57. From this investigation, it can be concluded that Azospirillum strains OAD-2 and OAD-11 can play an important role in the N nutrition of G. pulchella.  相似文献   

Anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate against white spot syndrome virus in freshwater crab (Paratelphusa hydrodromous)     

Karthikeyan  K.  Kumar  A. T. Manish  Harshini  D.  Pooja  G.  Daniel  J. Arul  Yasam  Pavani  Gracevictoria  G.  Priayanka  M.  Krishnaraj  P.  Vidya  R.  Devi  S. Asha  Reddy  Sabbasani Rajasekhara  Sudhakaran  Raja 《Aquaculture International》2022,30(2):989-998

Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet the issue remains unsolved. The present study was carried out to determine the anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate (C₁₃H₁₅N₂O₂Cl) against WSSV. The anti-viral activity of the synthetic compound was determined in freshwater crabs. Crabs were divided into three different experimental groups: healthy control groups (N.C.) received NTE buffer, positive control group (P.C.) crabs received WSSV, and treatment group crabs received WSSV along with synthetic weight compound. Experimental groups were observed for 30 days post-infection. Three different organs (gills, muscles, and head soft tissue (HST)) were dissected from all three groups and analyzed using molecular-based techniques, including polymerase chain reaction (PCR), Western blot, and histopathology. Clinical signs of WSSV were observed in the positive and N.C. groups; however, the treatment group showed a 100% survival rate. Confirmation was done using PCR, Western blot, and histopathology. These results demonstrated that the given synthetic compound has significant anti-viral activity against WSSV.

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Analysis of molecular variance and population structure in southern Indian finger millet genotypes using three different molecular markers     

Host Antony David Rajendran  Ramakrishnan Muthusamy  Antony Caesar Stanislaus  Thirugnanasambantham Krishnaraj  Sivasankaran Kuppusamy  Savarimuthu Ignacimuthu  Naif Abdullah Al-Dhabi 《Journal of Crop Science and Biotechnology》2016,19(4):275-283

The genetic relationship among 42 genotypes of finger millet collected from different geographical regions of southern India was investigated using random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR), and simple sequence repeats (SSR) markers. Ten RAPD primers produced 111 polymorphic bands. Five ISSR primers produced a total of 61 bands. Of these, 23 bands were polymorphic. The RAPD and ISSR fingerprints revealed 71.3 and 37.4% polymorphic banding patterns, respectively. Thirty-six SSR primers yielded 83 scorable alleles in which 62 were found to be polymorphic. Out of 36 SSR primers used, 14 primers (46.6%) produced polymorphic bands. The SSR primer UGEP7 produced a maximum number of six alleles. Mean polymorphic information content (PIC) of RAPD, ISSR and SSR were 0.44, 0.28, and 0.14, respectively. Molecular variances among the population were 2, 11, and 1% for RAPD, ISSR, and SSR markers, respectively. SSR produced 99% molecular variance within individuals. RAPD and ISSR markers produced a low level of molecular variance within individuals. The STRUCTURE (model-based program) analysis revealed that the 42 finger millet genotypes could be divided into a maximum of four subpopulations. Based on the Bayesian statistics, each RAPD and SSR marker produced three subpopulations (K=3), while ISSR marker showed four subpopulations (K=4). This study revealed that RAPD and SSR markers could narrow down the analysis of population structure and it may form the basis for finger millet breeding and improvement programs in the future.  相似文献   

Ecological and morphological characterisation of two rare and endemic wild edible <Emphasis Type="Italic">Cucumis</Emphasis> species (Cucurbitaceae) of Western Ghats of India     

K. Joseph John  Y. C. Roy  M. V. Krishnaraj  V. A. M. Nissar  M. Latha  K. V. Bhat 《Genetic Resources and Crop Evolution》2017,64(1):149-158

Cucumis silentvalleyi (Manilal, T. Sabu et P.J. Mathew) Ghebretinsae et Thulin and Cucumis indicus Ghebretinsae et Thulin are two rare narrow endemics of Western Ghats of India. Both have morphological resemblance but distinguishable by a few key characters like shape of the leaf, ovary and fruit, and chromosome number. While both are edible and non-bitter, their distribution is mutually exclusive, but overlapping with Cucumis sativus var. hardwickii (Royle) Alef. (the wild and feral form of C. sativus L.). Interspecific crossability studies indicate that both are related to each other and to a lesser extent to Cucumis setosus Cogn. and Cucumis melo L. Being incompletely described, a comparative account of their morphology, phenology, distribution, ecology and conservation are provided.  相似文献   

A new subspecies of <Emphasis Type="Italic">Momordica cochinchinensis</Emphasis> (Cucurbitaceae) from Andaman Islands,India     

K. Joseph John  Y. C. Roy  M. V. Krishnaraj  R. Asokan Nair  M. Deepu  M. Latha  K. V. Bhat  L. K. Bharathi 《Genetic Resources and Crop Evolution》2018,65(1):103-112

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On the taxonomic status,occurrence and distribution of <Emphasis Type="Italic">Cucumis hystrix</Emphasis> Chakrav. and <Emphasis Type="Italic">Cucumis muriculatus</Emphasis> Chakrav. (Cucurbitaceae) in India     

K. Joseph John  M. V. Krishnaraj  K. Pradheep  L. K. Bharathi  A. Suma  M. Latha  S. R. Yadav  K. V. Bhat 《Genetic Resources and Crop Evolution》2018,65(6):1687-1698

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Development of NILs from heterogeneous inbred families for validating the rust resistance QTL in peanut (Arachis hypogaea L.)     

Sharanabasappa B. Yeri  Kenta Shirasawa  Manish K. Pandey  M. V. C. Gowda  Vanhi Sujay  Manda Shriswathi  Hajisaheb L. Nadaf  Babu N. Motagi  S. Lingaraju  A. R. S. Bhat  Rajeev K. Varshney  P. U. Krishnaraj  Ramesh S. Bhat 《Plant Breeding》2014,133(1):80-85

Heterogeneous inbred families segregating for rust resistance were identified from the two crosses involving susceptible (TAG 24 and TG 26) and resistant (GPBD 4) varieties of peanut. Rust‐resistant (less than score 5) and rust‐susceptible (more than score 5) plants were identified in each HIF and evaluated under rust epiphytotic conditions. The set of plants belonging to the same HIF, but differing significantly in rust resistance, not in other morphological and productivity traits, was regarded as near‐isogenic lines (NILs). Largely, rust‐resistant NILs had GPBD 4‐type allele, and susceptible NILs carried either TAG 24 or TG 26‐type allele at the three SSR loci (IPAHM103, GM1536 and GM2301) linked to a major genomic region governing rust resistance. Comparison of the remaining genomic regions between the NILs originating from each of the HIFs using transposon markers indicated a considerably high similarity of 86.4% and 83.1% in TAG 24 × GPPBD 4 and TG 26 × GPBD 4, respectively. These NILs are useful for fine mapping and expression analysis of rust resistance.  相似文献