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1.
Ana E. González-Santiago Belinda Vargas-Guerrero Pedro M. García-López Alma L. Martínez-Ayala José A. Domínguez-Rosales Carmen M. Gurrola-Díaz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):134-140
Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders. 相似文献
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3.
Gustavo Pereira Valani Aline Fachin Martíni Laura Fernanda Simes da Silva Renata Cristina Bovi Miguel Cooper 《Soil Use and Management》2021,37(1):22-36
Integrated crop–livestock–forest is a promising strategy to improve soil quality. It comprises four different integrated farming systems: crop–livestock, crop–forest, forest–livestock and crop–livestock–forest. This work systematically reviewed studies about integrated crop–livestock–forest systems and soil quality. A total of 92 papers were retrieved from the Web of Science—Clarivate Analytics platform, and the following information was analysed: publication year, institution, region of the studied site, type of integrated system, soil type, tillage system, maximum soil depth and the soil quality indicators assessed. Most studies were published in the second half of the 2010s. Brazil is a prominent focus of research about soil quality and integrated crop–livestock–forest systems, with significant contribution from its central and southern regions. The Embrapa was the main publishing institution, present in over one‐third of the studies. Crop–livestock was the most common integrated system, Ferralsols was the most common soil group, and most of the studied soils were clayey. No tillage was the main tillage system. Most studies focused on the topsoil, assessing physical and/or chemical soil quality indicators. More emphasis on biological indicators of soil quality is required, as well as assessments integrating biological, physical and chemical indicators of soil quality. Future works should compare different integrated systems, including assessments deeper in the soil profile, especially in systems with the forest component, and also in sandy and silty soils. Soil quality indicators that have been rarely used should be further tested. Novel indicators should be added to better understand the promotion of soil quality by integrated crop–livestock–forest systems. 相似文献
4.
Marlene Cordero Pedro L. Ramos Lester Hernández Ana I. Fernández Ana L. Echemendía Rudy Peral Gloria González Daniel García Surey Valdés Ana Estévez Keren Hernández 《Phytoparasitica》2003,31(5):478-489
The presence of a begomovirus in potato plants with yellow mottle symptoms was determined for the first time in Cuba. The
incidence of typical begomovirus-like symptoms in potato plants in some regions of Havana province (Güira de Melena, San José
de las Lajas, Güines and Boyeros) during the growing seasons from 1992 to 1998 was in general low. However, in some cultivars
belonging to the National Program for Potato Genetic Improvement, the incidence reached 100%. Yield losses, determined in
1992 and 1994, ranged as high as 19% to 56.33% depending on the cultivar. Characterization of the causal agent was done by
light microscopy, host range (graft and mechanical transmission), DNA hybridizations, polymerase chain reaction, and restriction
fragment length polymorphism analysis. Nucleotide sequence of the amplified fragments revealed the presence ofTomato mottle Taino virus. The virus was transmittedvia tubers and has been detected in mixed infections withPotato virus X and withPotato leaf roll virus.
http://www.phytoparasitica.org posting Oct. 20, 2003. The first two authors contributed equally to this work. 相似文献
5.
Benjamin Brault DVM James Hoskinson DVM Laura Armbrust DVM George Milliken PhD 《Veterinary radiology & ultrasound》2004,45(4):298-304
The purpose of this study was to evaluate seven digital cameras for their ability to adequately capture quality radiographic images and their relative cost effectiveness. Radiographs of line pair (LP) phantoms (ranging from 0.6 to 16.6 LP/mm) and a 20-step contrast phantom were photographed at a near distance of 30.0 cm and a far distance (determined for each camera) at which a full 14 x 17 in film filled the viewfinder. Of the seven cameras tested, the Canon EOS-D30 consistently performed the best in LP/mm resolved (7.7 at close distance, 1.4 at far distance) and in contrast latitude (all 20 steps at both close and far distances). The Canon EOS-D30, determined to be the best camera used in this study for photographing radiographic images, is also the most expensive camera that was used. Two cameras, the Nikon Coolpix 995 and the Sony DSC-F707, were very close to the Canon in performance at considerably less cost. Certain inexpensive cameras may not be acceptable choices for teleradiology. 相似文献
6.
Kathryn C Gamble Michael M Garner Laura Krause Thomas P Alvarado 《Journal of zoo and wildlife medicine》2004,35(3):361-369
Two adult female rock hyraxes (Procavia capensis) at the Dallas Zoo were confirmed with spontaneous diabetes mellitus from 1997-2000, whereas a third animal with a similar clinical presentation never became hyperglycemic. The pancreas in all three animals showed pancreatic islet fibrosis (PIF). Retrospective examination of medical records for rock hyraxes acquired by this collection or born into it from 1991-2002 identified eight more animals affected with PIE All affected animals, including three males and eight females, were 1-7 yr of age and presented either with vague clinical signs of soft feces and rough hair coat or were acutely moribund or dead. Clinical pathology data was available for seven of the animals before onset of overt clinical signs and revealed inappropriate hyperglycemia in six, as well as elevated serum concentrations of creatine phosphokinase, amylase, and lipase in all seven animals. Pedigree evaluation did not support a familial pattern for PIE Review of the histopathology findings from nine other zoologic collections with rock hyrax deaths during the study period identified six institutions with 12 additional cases genetically unrelated to the incident collection. Histopathology and viral serology did not support an infectious cause. Analysis of serum anti-islet and anti-insulin antibodies did not suggest autoimmune disease, and none of the animals had known exposure to toxic substances. Limited nutritional analyses did not support a nutritional basis for the condition, and the cause for PIF remains unknown. 相似文献
7.
Barriers to and enablers of implementing antimicrobial stewardship programs in veterinary practices 下载免费PDF全文
8.
Daniel A. Truchado Michaël A. J. Moens Sergio Callejas Javier Pérez-Tris Laura Benítez 《Veterinary research communications》2018,42(2):111-120
Papillomaviruses are non-enveloped, DNA viruses that infect skin and mucosa of a wide variety of vertebrates, causing neoplasias or simply persisting asymptomatically. Avian papillomaviruses, with six fully sequenced genomes, are the second most studied group after mammalian papillomaviruses. In this study, we describe the first oral avian papillomavirus, detected in the tongue of a dead Yorkshire canary (Serinus canaria) and in oral swabs of the same bird and other two live canaries from an aviary in Madrid, Spain. Its genome is 8,071 bp and presents the canonical papillomavirus architecture with six early (E6, E7, E1, E9, E2, E4) and two late open reading frames (L1 and L2) and a long control region between L1 and E6. This new avian papillomavirus L1 gene shares a 64% pairwise identity with FcPV1 L1, so it has been classified as a new species (ScPV1) within the Ethapapillomavirus genus. Although the canary died after showing breathing problems, there is no evidence that the papillomavirus caused those symptoms so it could be part of the oral microbiota of the birds. Hence, future investigations are needed to evaluate the clinical relevance of the virus. 相似文献
9.
María Teresa Tejedor-Junco Margarita González-Martín Estefanía Bermeo-Garrido Rebeca Villasana-Loaiza Elena Carretón-Gómez 《Veterinary research communications》2018,42(3):227-232
Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance. 相似文献