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1.
T. Winkel H. D. Bertero P. Bommel J. Bourliaud M. Chevarría Lazo G. Cortes P. Gasselin S. Geerts R. Joffre F. Léger B. Martinez Avisa S. Rambal G. Rivière M. Tichit J. F. Tourrand A. Vassas Toral J. J. Vacher M. Vieira Pak 《Journal of Agronomy and Crop Science》2012,198(4):314-319
Reviewing the situation of quinoa production in southern Bolivia, Jacobsen (2011, J. Agron. Crop Sci. 197: 390) argues that the booming export market has a negative effect on the environment and on the home consumption of quinoa, thereby leading to an environmental disaster in the region. In view of the scarcity of scientific knowledge on the rapid social and environmental dynamics in the region, we consider that Jacobsen’s review misrepresents the situation of quinoa production in southern Bolivia. Specifically, we argue that (i) the data presented by Jacobsen (2011, J. Agron. Crop Sci. 197: 390) do not support any drop in quinoa crop yield supposed to reflect soil degradation and (ii) his demonstration regarding home consumption of quinoa is ill‐founded from both a nutritional and a cultural point of view. We suggest that the diffusion of the arguments exposed by Jacobsen (2011, J. Agron. Crop Sci. 197: 390), because of their flaws, might have strong negative impacts on those concerned with sustainable food production and fair‐trade with developing countries. We conclude that, rather than reinforced agro‐technical controls on local farmers, the rising competition in the international quinoa market requires a shift towards an ethical economy and ethical research cooperation with quinoa producers. 相似文献
2.
Xuezhi Zhu D. Allen Davis Luke A. Roy T. M. Samocha J. P. Lazo 《Journal of the World Aquaculture Society》2013,44(3):396-404
Three sources of soybean meal (SBM) were biochemically characterized and evaluated in diets for juvenile Litopenaeus vannamei. Three biological techniques were utilized to evaluate the nutritional quality of the meals including an outdoor growth trial as well as determination of in vivo apparent digestibility and in vitro digestibility including pepsin digestibility (0.0002%), pH Stat degree of hydrolysis, and immobilized digestive enzyme assay (IDEA). The growth trial was conducted over a 10‐wk period using soybean meal as the primary protein source with each meal being included at 54–58% of the diet based on an equal protein inclusion. At the conclusion of the growth trial there were no significant differences between the test diets indicating equivalent biological availability under practical conditions. The in vivo digestibility trial utilized chromic oxide as the inert marker and 70:30 replacement techniques resulted in typical results. Apparent protein digestibility (APD) and apparent energy digestibility (AED) of the diets ranged from 66.8 to 80.2% and 65.4 to 74.7%, respectively. Ingredient digestibility values for protein and energy ranged from 78.8 to 93.5% and 60.5 to 85.0%, respectively. One of the three SBM evaluated had numerically lower protein digestibility and significantly lower energy digestibility than the other two meals . 相似文献
3.
Juan Pablo Lazo Robert P. Romaire Robert C. Reigh 《Journal of the World Aquaculture Society》1998,29(4):441-450
Abstract— In vitro enzyme assays are rapid, inexpensive techniques for estimating protein digestibility of feed ingredients. Three assays—the Lazo single-enzyme assay with porcine trypsin; the Hsu multi-enzyme assay with porcine trypsin, α-chymotrypsin, and peptidase; and the Satterlee multi-enzyme assay with porcine trypsin, α-chymotrypsin, peptidase, and bacterial protease—were used to estimate relative protein digestibility (RPD) of selected feed ingredients used in diets for the Pacific white shrimp Penaeus vannamei. Ingredients tested were casein, gelatin, rice bran, shrimp meal, soybean meal, wheat gluten, and six varieties of fish meal. A highly significant, inverse, linear relationship existed between final pH in each of the enzyme assays and in vivo apparent protein digestibility of the ingredient in P. vannamei feeding trials. Ranking of RPD coefficients obtained in the Lazo single-enzyme assay followed closely the ranking of published in vivo apparent protein digestibility coefficients for the same ingredients in P. vannamei digestibility trials. Results indicated that a single-enzyme assay with porcine trypsin identified differences in protein digestibility of feedstuffs in vitro as well as the two multi-enzyme assays tested. The porcine-trypsin, single-enzyme assay appears to be a potentially useful tool for evaluating protein quality and relative protein digestibility of feedstuffs for P. vannamei. 相似文献
4.
The temporal evolution of chymotrypsin activity during early ontogeny of laboratory reared red drum larvae was accomplished using a combination of biochemical assays and electrophoretic methods (substrate SDS-PAGE). Optimal functional conditions for chymotrypsin were also determined. Chymotrypsin activity was first detected prior to the onset of exogenous feeding. Total chymotrypsin activity increased with age and standard length. Specific activity was greatest on day 10 post-hatch. Maximal chymotrypsin activity was observed at 50 °C, pH 7.8, and Ca2+ concentration of 25 mM. Using substrate gel electrophoresis and specific inhibitors the molecular weight of red drum chymotrypsin was estimated to be 26–27 kD. Our results indicate that the digestive system of red drum larvae is capable of alkaline proteolysis before first feeding and suggest that chymotrypsin may have potential as an indicator of nutritional condition. 相似文献
5.
Molecular mapping and characterization of traits controlling fiber quality in cotton 总被引：34，自引：1，他引：34
Cotton (Gossypium spp) is the world's leading natural fiber crop. Genetic manipulation continues to play a key role in the improvement of fiber
quality properties. By use of DNA-based molecular markers and a polymorphic mapping population derived from an inter specific
cross between TM-1 (G. hirsutum) and 3-79 (G. barbadense), thirteen quantitative trait loci (QTLs) controlling fiber quality properties were identified in 3-79, an extra long staple
(ELS) cotton. Four QTLs influenced bundle fiber strength, three influenced fiber length, and six influenced fiber fineness.
These QTLs were located on different chromosomes or linkage groups and collectively explained 30% to 60%of the total phenotypic
variance for each fiber quality property in the F2 population. The effects and modes of action for the individual QTLs were characterized with 3-79 alleles in TM-1 genetic
background. The results indicated more recessive than dominant, with much less additive effect in the gene mode. Transgressive
segregation was observed for fiber fineness that could be beneficial to improvement of this trait. Molecular markers linked
to fiber quality QTLs would be most effective in marker-assisted selection (MAS) of these recessive alleles in cotton breeding
programs.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
6.
7.
Magali Zacarias-Soto Benjamín Barón-Sevilla Juan P. Lazo 《Fish physiology and biochemistry》2013,39(5):1331-1339
Studies aimed to assess the digestive physiology of marine fish larvae under culture conditions are important to further understand the functional characteristics and digestive capacities of the developing larvae. Most studies to date concentrate on intestinal lumen digestion and little attention to the absorption process. Thus, the objectives of this study were to histochemically detect and quantify some of the enzymes responsible for absorption and intracellular digestion of nutrients in the anterior and posterior intestine of California halibut larvae. Alkaline and acid phosphatases were detected from the first days post-hatch (dph). Alkaline phosphatase maintained a high level of activity during the first 20 dph in both intestinal regions. Thereafter, a clear intestinal regionalization of the activity was observed with the highest levels occurring in the anterior intestine. Acid phosphatase activity gradually increased in both intestinal regions during development, and a regionalization of the activity was not observed until late in development, once the ocular migration began. Highest levels were observed in the anterior intestine at the end of metamorphosis concomitant with the stomach development. The results from this study show some morphological and physiological changes are occurring during larval development and a clear regionalization of the absorption process as the larvae develops. These ontological changes must be considered in the elaboration of diets according to the digestive capacity of the larvae. 相似文献
8.
Winfried Schröder Stefan Nickel Simon Schönrock Roman Schmalfuß Werner Wosniok Michaela Meyer Harry Harmens Marina V. Frontasyeva Renate Alber Julia Aleksiayenak Lambe Barandovski Oleg Blum Alejo Carballeira Maria Dam Helena Danielsson Ludwig De Temmermann Anatoly M. Dunaev Barbara Godzik Katrin Hoydal Zvonka Jeran Gunilla Pihl Karlsson Pranvera Lazo Sebastien Leblond Jussi Lindroos Siiri Liiv Sigurður H. Magnússon Blanka Mankovska Encarnación Núñez-Olivera Juha Piispanen Jarmo Poikolainen Ion V. Popescu Flora Qarri Jesus Miguel Santamaria Mitja Skudnik Zdravko Špirić Trajce Stafilov Eiliv Steinnes Claudia Stihi Ivan Suchara Lotti Thöni Hilde Thelle Uggerud Harald G. Zechmeister 《Annals of Forest Science》2017,74(2):31
Key message
Moss surveys provide spatially dense data on environmental concentrations of heavy metals and nitrogen which, together with other biomonitoring and modelling data, can be used for indicating deposition to terrestrial ecosystems and related effects across time and areas of different spatial extension.Context
For enhancing the spatial resolution of measuring and mapping atmospheric deposition by technical devices and by modelling, moss is used complementarily as bio-monitor.Aims
This paper investigated whether nitrogen and heavy metal concentrations derived by biomonitoring of atmospheric deposition are statistically meaningful in terms of compliance with minimum sample size across several spatial levels (objective 1), whether this is also true in terms of geostatistical criteria such as spatial auto-correlation and, by this, estimated values for unsampled locations (objective 2) and whether moss indicates atmospheric deposition in a similar way as modelled deposition, tree foliage and natural surface soil at the European and country level, and whether they indicate site-specific variance due to canopy drip (objective 3).Methods
Data from modelling and biomonitoring atmospheric deposition were statistically analysed by means of minimum sample size calculation, by geostatistics as well as by bivariate correlation analyses and by multivariate correlation analyses using the Classification and Regression Tree approach and the Random Forests method.Results
It was found that the compliance of measurements with the minimum sample size varies by spatial scale and element measured. For unsampled locations, estimation could be derived. Statistically significant correlations between concentrations of heavy metals and nitrogen in moss and modelled atmospheric deposition, and concentrations in leaves, needles and soil were found. Significant influence of canopy drip on nitrogen concentration in moss was proven.Conclusion
Moss surveys should complement modelled atmospheric deposition data as well as other biomonitoring approaches and offer a great potential for various terrestrial monitoring programmes dealing with exposure and effects.9.
Morphological development and allometric growth of yellowtail kingfish Seriola lalandi V. larvae under culture conditions 
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下载免费PDF全文 Emmanuel Martínez‐Montaño Karina González‐Álvarez Juan Pablo Lazo Juan Manuel Audelo‐Naranjo Antonio Vélez‐Medel 《Aquaculture Research》2016,47(4):1277-1287
Morphological development and allometric growth patterns of Seriola lalandi larvae were assessed to characterize normal growth patterns under culture conditions. Early ontogenetic stages of yellowtail kingfish exhibited an exponential growth in terms of standard length as a function of age. Five development stages were characterized from hatching to the juvenile stage: larval stage I (0–2 days post hatch, dph) with endogenous feeding, characterized by a small yolk sac, unpigmented eyes, primordial finfold surrounding the body and a closed mouth; larval stage II (2–15 dph) characterized by mouth opening, complete pigmentation of eyes and the beginning of the exogenous feeding; subsequently, in the larval stage III (15–25 dph) the posterior tip of notochord of the larvae bended upward and the first rays appeared in fins, concomitant with a change in swimming behaviour; thereafter, larval stage IV (post‐flexion stage; 25–30 dph) began when larvae resembled in morphology to a juvenile organism; however, caudal and dorsal fins were not completely development. Lastly, the juvenile stage was reached 30 dph characterized by a morphology and fin structures similar to those of the adults. Growth and development of structures and organs associated with vital functions such as feeding, sensorial and breathing systems seemed to be more critical previous to 23 dph, which was reflected with a positive allometric growth of head and eyes during this period. The results from this study can be used as a tool‐guide to assess normal development in larval research with S. lalandi to improve existing rearing protocols in hatchery production. 相似文献
10.
Effect of Two Novel Experimental Microdiet Types on Growth,Survival, and Pigmentation during the Weaning Period of the Fine Flounder,Paralichthys adspersus,Larvae 下载免费PDF全文
下载免费PDF全文 Lucas Orihuela Melissa Montes Joel Linares Angélica Castro Lili Carrera Juan P. Lazo 《Journal of the World Aquaculture Society》2018,49(4):770-779
The aim of this study was to evaluate two new types of experimental weaning microdiets for fish larvae, using the same formulation, but with different manufacturing processes, microextrusion marumerization (MEM) and particle‐assisted rotational agglomeration (PARA). Both microdiets were compared to a commercial microdiet, Otohime? (OTO), during the weaning of the fine flounder, Paralichthys adspersus . Weaning was achieved by cofeeding with live food from 40 to 50 d after hatch (d.a.h.). Thereafter, only the microdiets were fed to the end of the study (60 d.a.h.), where the total length, dry weight, and survival rates were 15.9 ± 3.20, 14.3 ± 1.72, and 14.4 ± 2.28 mm; 8.83 ± 3.40, 5.53 ± 2.85, and 7.10 ± 3.56 mg; 18.1, 16.3, and 15.2%, for OTO, MEM, and PARA, respectively. The dry weight and total length were significantly higher (P < 0.05) for larvae fed OTO compared with those fed MEM. There were no differences between the other comparisons. Based on these results, any of the two experimental microdiets can be used for future investigations. However, we recommend the PARA microdiets because the manufacturing process produces less‐dense and smaller particle size diets, reducing sinking rates and increasing the probability of the larvae in detecting and ingesting the diet. 相似文献