Cyclooxygenase ‐2 Expression in Mammary Tumors in Dogs and its Correlation to Histologic and Biologic Behavior     

A. B. De Nardi  C. R. Daleck  C. H. M.  Souza  R. L. Amorin  S. Rodaski  C. Calderon  R. Torres 《Veterinary and comparative oncology》2005,3(1):56-57

Introduction:  Cyclooxygenase‐2 (Cox‐2) is the inducible form and the rate‐limiting enzyme, for conversion of arachidonic acid to prostaglandins. Cox‐2 overexpression, common in carcinomas, is associated with increased growth rate, resistance to apoptosis, angiogenesis, and overall, both local and distant aggressive behavior. Cox‐2 overexpression has been detected in human and canine mammary tumors (MTs). Histopathology of canine MT is not always predictive of biologic behavior, and anecdotally, only 50% of the malignant MTs are expected to metastasize. We hypothesize that Cox‐2 expression correlates with aggressive behavior.
Methods:  This retrospective study evaluated 48 bitches, presented for excision of MT between 2000 and 2003 at FMVZ de Botucatu‐UNESP, Brasil. Follow‐up varied from 18 months to 24 months and included physical examination and thoracic radiographs. Histopathologic examination was performed in all tumors, as well as in metastatic lesions when detected in the follow‐up period. Immunohistochemistry was used to detect expression of Cox‐2 in paraffin blocks (Rabbit polyclonal anti‐PGHS‐2. Oxford Biomedical). 10 adenomas, 10 carcinomas, 10 benign mixed tumors, 10 malignant mixed tumors and 8 cases of primary carcinomas and their metastatic lesions.
Results:  Expression of Cox‐2 varied among groups. Adenomas (32.1%), mixed benign tumors (38%), carcinomas (60.3%), malignant mixed tumors (65.8%), and metastatic carcinomas (81.25%) and their metastatic lesions (84.35%). Statistically significant differences (p < 0.05) were observed between the benign and malignant counterparts and between carcinomas and metastatic carcinomas.
Conclusions:  Cox‐2 expression correlates with both histologic and biologic behavior in mammary carcinomas, and may serve as a predictor of metastatic potential.  相似文献   

Patch test reactions to house dust mites in dogs with atopic dermatitis     

S. A. F. Nogueira  S. M. F. Torres  K. Horne  C. Jessen 《Veterinary dermatology》2004,15(Z1):4-4

The purpose of this study was to determine the percentage of dogs with spontaneous atopic dermatitis that show a positive patch test reaction to a commercially available 20% house dust mites mixture containing equal parts of Dermatophagoides farinae and Dermatophagoides pteronyssinus in white petrolatum. In addition, we evaluated whether skin reactions induced after the epicutaneous application of house dust mites were clinically and histologically similar to naturally developed skin lesions of dogs with atopic dermatitis. Furthermore, we investigated if the reactions induced by house dust mites were true allergic reactions by comparing them to atopic lesional skin and to patch test reactions induced by an irritant substance (sodium lauryl sulphate). White petrolatum alone and nonlesional skin sites were used as negative controls. Macroscopic and microscopic evaluations of the patch test and control sites were performed in a blinded fashion at 48 and 72 h after patch test application. Microscopic results were evaluated in a qualitative and quantitative manner. A chi‐square test for homogenicity was used for the quantitative analysis to compare the proportion of each dermal inflammatory cell type among positive histopathological tested sites. P values ≤ 0.05 were considered significant. The study included 12 healthy nonatopic dogs and 13 dogs with nonseasonal atopic dermatitis. None of the nonatopic dogs reacted to house dust mites and white petrolatum. Ten (77%) of the 13 atopic dogs reacted macroscopically and histopathologically to house dust mites. Macroscopic reactions induced by house dust mites were characterized by erythema, oedema and papules. The macroscopic reactions induced by house dust mites were identical to lesional skin in 20% of the dogs and identical to reactions induced by sodium lauryl sulphate in 40% of the dogs. Qualitative histopathological findings showed that the reactions induced by house dust mites were similar to atopic lesional skin in 80% of the dogs and were similar to sodium lauryl sulphate in 20% of the dogs. Quantitative analyses showed that the proportion of neutrophils in reactions induced by sodium lauryl sulphate was significantly higher (P < 0.05) compared to house dust mites reactions, which could be a differentiator factor between an allergic and an irritant reaction. These results showed that the epicutaneous application of house dust mites in dogs with atopic dermatitis induced histopathological lesions similar to spontaneous atopic lesions in dogs. Therefore, this study demonstrated that house dust mites penetrated the skin of dogs with atopic dermatitis and induced an inflammatory response that resembled a true allergic reaction. Funding: Small Companion Animal Grant, University of Minnesota.  相似文献   

Bovine viral diarrhea virus subgenotype 1b in water buffalos (Bubalus bubalis) from Brazil     

Paixão  Sonália Ferreira  Fritzen  Juliana Torres Tomazi  Crespo  Sarah Elizabeth Izzo  de Moraes Pereira  Helder  Alfieri  Alice Fernandes  Alfieri  Amauri Alcindo 《Tropical animal health and production》2018,50(8):1947-1950

Tropical Animal Health and Production - Serological studies have characterized the presence of the bovine viral diarrhea virus (BVDV) infection in water buffalo herds worldwide. However, the...  相似文献   

Stem cell factor supports migration in canine mesenchymal stem cells     

Nathaly Enciso  Luciana L. K. Ostronoff  Guillermo Mejías  Leticia G. León  María Luisa Fermín  Elena Merino  Cristina Fragio  Luis Avedillo  Concepción Tejero 《Veterinary research communications》2018,42(1):29-38

Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25–30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration.  相似文献   

Subcutaneous administration of ciclosporin in 11 allergic cats – a pilot open‐label uncontrolled clinical trial          下载免费PDF全文

Sandra N. Koch  Sheila M. F. Torres  Sandra Diaz  Sophie Gilbert  Aaron Rendahl 《Veterinary dermatology》2018,29(2):107-e43

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Evaluation of alternative sanitizers to chlorine disinfection for reducing foodborne pathogens in fresh-cut apple     

Maribel Abadias  Isabel Alegre  Josep Usall  Rosario Torres  Inmaculada Viñas 《Postharvest Biology and Technology》2011,59(3):289-297

The risk of undesirable by-products from chlorine disinfection in fresh-cut industries, together with its limited efficacy, has led to a search for alternative agents. The aim of this study was to test several alternative putative antimicrobial substances to reduce Escherichia coli O157:H7, Salmonella spp. and Listeria spp. populations on fresh-cut apple. Carvacrol, vanillin, peroxyacetic acid, hydrogen peroxide, N-acetyl-l-cysteine and Citrox were selected for their results in in vitro assays against E. coli O157:H7 and Listeria spp., to be tested on fresh-cut apple plugs. Apple flesh was inoculated by dipping in a suspension of a mix of the studied pathogens at 10⁶ cfu mL^?1, and then treated with the antimicrobial substances. All treatments were compared to deionized water and a standard sodium hypochlorite treatment (SH, 100 mg L^?1, pH 6.5). Pathogen population on apple plugs was monitored for up to 6 days at 10 °C. Bacterial reductions obtained by peroxyacetic acid (80 and 120 mg L^?1), vanillin (12 g L^?1), hydrogen peroxide (5, 10, 20 mL L^?1) and N-acetyl-l-cysteine (5 and 10 g L^?1) were similar or higher than reduction obtained by SH. In addition, bacterial populations were maintained at low levels throughout storage. No cells of any of the pathogens were detected in the peroxyacetic acid, hydrogen peroxide, Citrox and SH washing solutions after apple treatment. Peroxyacetic acid, hydrogen peroxide and N-acetyl-l-cysteine could be potential disinfectants for the fresh-cut industry as an alternative to chlorine disinfection. However, their effect on sensory quality and effectiveness under commercial processing conditions should be evaluated.  相似文献   

Microdialysis measurements of equine lamellar perfusion and energy metabolism in response to physical and pharmacological manipulations of blood flow          下载免费PDF全文

C. E. Medina‐Torres  C. Underwood  C. C. Pollitt  E. M. Castro‐Olivera  M. P. Hodson  D. W. Richardson  A. W. van Eps 《Equine veterinary journal》2016,48(6):756-764

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Predation performance and survival of susceptible and pyrethroid-resistant Eriopis connexa Germar (Coleoptera: Coccinellidae) to insecticides used in brassica crops     

Rogério Lira  Deividy Vicente do Nascimento  Paulo Roberto Ramos Barbosa  Alvin M. Simmons  Jorge Braz Torres 《Pest management science》2023,79(8):2704-2712

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The development of the normal Schirmer tear test results during the dog's first year of life     

Manuella Oliveira Borges de Sampaio  Bret A. Moore  Nathália Mendonça de Seabra  Mariza Bortolini  Thayane Cristina Vieira  Camilla Bolmann Martins  Ana Leticia Groszewicz de Souza  Camila Marinelli Martins  Fabiano Montiani-Ferreira 《Veterinary ophthalmology》2023,26(3):184-190

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Effect of sirolimus on insulin dynamics in horses     

Demia J. de Tonnerre  Carlos E. Medina Torres  Darko Stefanovski  Mary A. Robinson  Kate L. Kemp  François-René Bertin  Andrew W. van Eps 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):703-712

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