Rabies virus infection in Aedes pseudoscutellaris cells: a study on receptorial structures     

L Seganti  F Superti  L Sinibaldi  M Marchetti  S Bianchi  N Orsi 《Comparative immunology, microbiology and infectious diseases》1991,14(3):265-275

Rabies virus is able to infect in vitro a wide range of homeothermic and poikilothermic cells but little is known about its multiplication in arthropod cells. In this research the infection of rabies virus in Aedes pseudoscutellaris cells, a mosquito cell line susceptible to mosquito-borne viruses, was studied. After 60 days of incubation at 26 degrees C up to 70% of infected cells showed the synthesis of both viral nucleocapsid and envelope antigens, although viral yield and cell damage could not be detected. Research performed in order to investigate the role of membrane carbohydrate moieties in rabies virus-mosquito cell interaction suggested the participation of galactose and N-acetylglucosamine whereas sialic acid, known to be a rabies binding site in many homeothermic cell lines, was not involved.  相似文献   

Brewery residues as a source of organic carbon in Litopenaeus schmitti white shrimp farms with BFT systems     

Michelle Midori Sena Fugimura  Helaine dos Reis Flor  Emanuela Paula de Melo  Tiago Viana da Costa  Wilson Wasielesky Jr.  Lidia Miyako Yoshii Oshiro 《Aquaculture International》2015,23(2):509-522

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Some observations on the morphology of the pigeon's seminiferous epithelium cells     

A M Orsi  M C Mercadante  E Dias  S Mello Dias  C A Vicentini 《Anatomia, histologia, embryologia》1984,13(4):327-332

A light microscopic examination of the seminiferous epithelium of the pigeon was carried out. Comparisons are made between the spermiogenic stages of the pigeon and that of the domestic fowl and mammals.  相似文献   

Inhibition of inducible nitric oxide synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits     

Rossi A  Rigano D  Pergola C  Formisano C  Basile A  Bramanti P  Senatore F  Sautebin L 《Journal of agricultural and food chemistry》2007,55(13):5053-5061

Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor kappaB (NF-kappaB) and/or mitogen-activated protein kinase (MAPK) activation.  相似文献   

Reduced-calorie Avocado Paste Attenuates Metabolic Factors Associated with a Hypercholesterolemic-high Fructose Diet in Rats     

María Elena Pahua-Ramos  Leticia Garduño-Siciliano  Lidia Dorantes-Alvarez  German Chamorro-Cevallos  Julieta Herrera-Martínez  Obed Osorio-Esquivel  Alicia Ortiz-Moreno 《Plant foods for human nutrition (Dordrecht, Netherlands)》2014,69(1):18-24

The objective of this study was to evaluate the effect of reduced-calorie avocado paste on lipid serum profile, insulin sensitivity, and hepatic steatosis in rats fed a hypercholesterolemic-high fructose diet. Thirty five male Wistar rats were randomly separated in five groups: Control group (ground commercial diet); hypercholesterolemic diet plus 60 % fructose solution (HHF group); hypercholesterolemic diet plus 60 % fructose solution supplemented with avocado pulp (HHF+A group); hypercholesterolemic diet plus 60 % fructose solution supplemented with reduced-calorie avocado paste (HHF+P group); and hypercholesterolemic diet plus 60 % fructose solution supplemented with a reduced-calorie avocado paste plus fiber (HHF+FP group). The A, P, and FP were supplemented at 2 g/kg/d. The study was carried out for seven weeks. Rats belonging to the HHF group exhibited significantly (P?≤?0.05) higher total cholesterol, triglycerides, and insulin levels in serum as well as lower insulin sensitivity than the control group. Supplementation with reduced-calorie avocado paste showed a significant (P?≤?0.05) decrease in total cholesterol (43.1 %), low-density lipoprotein (45.4 %), and triglycerides (32.8 %) in plasma as well as elevated insulin sensitivity compared to the HHF group. Additionally, the liver enzymes alanine aminotransferase and aspartate aminotransferase decreased significantly in the HHF-P group (39.8 and 35.1 %, respectively). These results are likely due to biocompounds present in the reduced-calorie avocado paste, such as polyphenols, carotenoids, chlorophylls, and dietary fibre, which are capable of reducing oxidative stress. Therefore, reduced-calorie avocado paste attenuates the effects of a hypercholesterolemic-high fructose diet in rats.  相似文献   

The analysis of PAHs nitrogen derivatives in the sewage sludges of upper Silesia, Poland     

Danuta Bodzek  Beata Janoszka  Lidia Warzecra 《Water, air, and soil pollution》1996,89(3-4):417-427

The dimethylformamide extracts from sewage sludges from a chosen sewage — treatment plant in highly industrialized region of Poland were investigated. Using the column chromatography with Al₂O₃ and silicic acid as the stationary phases, the fraction of azaarenes has been separated. GC-MS analysis of azaarenes fraction made it possible to identify 10 azaarenes and several of their alkyl derivatives, which dominate in the organic matter extracted from sewage sludges. The silicic acid column chromatography in connection with the reduction of nitroarenes and derivatization with pentafluoropropionic acid anhydride yielded an enriched nitrofraction. Nitroarenes present in this fraction as amide derivatives were also analysed by GGMS method. There was found presence of 3 nitroarenes and their methyl derivatives.  相似文献   

Influence of Organic Amendments on the Accumulation of 137Cs and 90Sr from Contaminated Soil by Three Grass Species     

James A. Entry  Lidia S. Watrud  Mark Reeves 《Water, air, and soil pollution》2001,126(3-4):385-398

Bahia grass (Paspalum notatum), johnson grass (Sorghum halpense) and switchgrass (Panicum virginatum) werecompared for their ability to accumulate ¹³⁷Csand ⁹⁰Sr from three different contaminated soilsin the presence and absence of either sphagnum peator poultry litter amendments. Above-ground plantbiomass did not differ between plants that were notexposed to these radionuclides and those that wereexposed to soil containing ¹³⁷Cs or ⁹⁰Sr.After three harvests, bahia, johnson and switchgrassplants accumulated from 17.2 to 67.3% of the¹³⁷Cs and from 25.1 to 61.7% of the ⁹⁰Sradded to the soil. Poultry litter and peat mossamendments increased aboveground plant biomass,activity of ¹³⁷Cs or ⁹⁰Sr in plant tissue, %accumulation of ¹³⁷Cs or ⁹⁰Sr from soil andthe plant bioconcentration ratio at each harvestcompared to the control (no amendment) treatment. Thegreatest increases in plant biomass, and radionuclideaccumulation were observed with poultry litter foreach of the three grass species. Johnson grass hadgreater aboveground plant biomass, activity of¹³⁷Cs and ⁹⁰Sr in plant tissue, %accumulation of ¹³⁷Cs or ⁹⁰Sr from soil andbioconcentration ratio in each soil amendment, at eachharvest compared to bahia and switchgrass. Thegreatest accumulation of ¹³⁷Cs and ⁹⁰Sr wasmeasured in johnson grass grown in soil that wasamended with poultry litter. These results suggestthat plant species selection and agronomic practicesmay need to be considered to maximize phytoremediationof radionuclide contaminated soils.  相似文献   

Consideraciones sobre el sistema urinario del Gallus gallus– Indian River; I. Formación y tributaris del uréter     

Por S. Mello Dias  P. Pinto Silva  V. J. M. Campos  A. M. Orsi  M. C. Oliveira Dayoub  R. M. Define 《Anatomia, histologia, embryologia》1983,12(4):347-352

In the literature reviewed, there are certain differences of opinion with regard to the formation and tributareis to the ureter. Therefore, dissection of 40 fowls injected with neoprene latex, k neoprene latex and celobar (radiopaque mass) and vinyl acetate casts through the ureter and blood vessels revealed the formation and disposition of the tributaries to the ureter, and its origin and number of collectors. The results were described in relation to the renal lobes.  相似文献   

Morphometric Analysis of the Intestine of Domestic Quails (Coturnix coturnix japonica) Treated with Different Levels of Dietary Calcium     

K. P. Aptekmann  S. M. Baraldi Artoni  M. A. Stefanini  & M. A. Orsi 《Anatomia, histologia, embryologia》2001,30(5):277-280

The aim of this study was to perform a morphometric analysis of the various parts of the intestine of the domestic quail. Twenty-four female quails (Coturnix coturnix japonica) aged 37 weeks were used and accommodated in laying cages for 12 weeks. Each group was fed a standardized diet containing different quantities of calcium: 2.0%, 2.5%, 3.0% and 3.5%. The birds were weighed, killed, and samples of 1 cm were collected from the duodenum, jejunum and ileum and submitted to the histological routine. The sections obtained were stained in haematoxylin & cosin (H&E). For morphometric analysis, 30 villi and 30 crypts of each segment of the small intestine were measured in order to determine the height and area of the villi, as well as the depth of the crypts. The results showed that although the integrity of the gastrointestinal tract was maintained in all the birds treated with the different calcium levels, a calcium level of 3.0% showed the most promise. as the levels of 2.0% and 2.5% did not cause any alteration in the intestinal tract. Furthermore, a calcium level of 3.5% led to a significant reduction in the height of the villosities, and in consequence reduced the digestive and absorptive capabilities.  相似文献   

Accumulation and depletion kinetics of erythromycin in rainbow trout (Oncorhynchus mykiss)     

Vendrell D  Serarols L  Balcázar JL  de Blas I  Gironés O  Múzquiz JL  Ruiz-Zarzuela I 《Preventive veterinary medicine》2012,105(1-2):160-163

Erythromycin is an antimicrobial agent recommended for the control and treatment of diseases caused by gram-positive bacteria. Few studies, however, have determined the metabolic and pharmacokinetic aspects of this antimicrobial agent in fish. The aim of the present study, therefore, was to determine the accumulation and depletion time of erythromycin after administration of medicated feed containing 52 mg kg(-1) body weight day(-1) for 8 days in rainbow trout (Oncorhynchus mykiss). Results were analyzed following the European Agency for Evaluation of Medicinal Products guidelines. We measured a withdrawal time of 187°C-day (°C-day=water temperature×days), lower than the value (500°C-day) recommended by Council Directive 2004/28/EC for veterinary medicinal products. Our results provide data to establish therapeutic regimens for the use of erythromycin in aquaculture.  相似文献