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Masafumi KATAYAMA Tomokazu FUKUDA Toshihiro OKAMURA Eisaku SUZUKI Katsuo TAMURA Yuuko SHIMIZU Yoshihito SUDA Keiichi SUZUKI 《Animal Science Journal》2011,82(2):274-281
In pig production, dietary additive antibiotics are usually used for growth stimulation and disease prevention, although there is public concern about the increased incidence of resistant antibiotics and food safety. It is possible that such antibiotics might be replaced by naturally derived products such as seaweed and licorice. In this study, we evaluated the effect of dietary addition of seaweed and licorice on enhancing the immune function in swine. The animals of each group (eight animals per group) were sensitized at day 42 and 49, and the immunoglobulin production and the expression of cytokines were detected by the ELISA and real‐time PCR. As the results, saliva IgA production of the seaweed‐treated group increased around five times compared to that of control (day 56). Delayed hypersensitivity reaction and IgG production of the seaweed‐treated group increased around 1.8–2.0 times. In addition, enhanced saliva IgA production was detected at day 50 (around two times) and day 51 (around five times) by the licorice treatment, and lower expression level of tumor necrosis factor‐α messenger RNA at day 51 (around 1/25) was observed in the licorice treatment. We conclude that the replacement of antibiotics by naturally derived dietary additives might be feasible for immune system enhancement. 相似文献
2.
Change of plasma insulin-like growth factor-1 (IGF-1) concentration with early growth in Japanese beef cattle 总被引：1，自引：0，他引：1
Yoshihito SUDA Kentaro NAGAOKA Kazuki NAKAGAWA Takashi CHIBA Fumihiro YUSA Hisashi SHINOHARA Akira NIHEI Toshihiro YAMAGISHI 《Animal Science Journal》2003,74(3):205-210
The aim of the present study was to examine the change in plasma insulin‐like growth factor‐1 (IGF‐1) concentration with early growth, changes of bodyweight (BW) and relative dairy gain (RDG) in the pre‐ (PRW) and postweaning periods (POW) in Japanese beef cattle, and relationships with metabolites. A total of 33 calves, 22 Japanese black, 6 Japanese shorthorn and 5 of their crossbreed were studied. Insulin‐like growth factor‐1 and metabolite (glucose, triacylglycerol, nonesterified fatty acid) levels in the plasma, from jugular vein blood taken every month, were measured along with BW. Insulin‐like growth factor‐1 in POW increased dramatically with increase of BW (P < 0.05), and the correlation was positive at 0.52 (P < 0.01). Glucose levels correlated significantly with BW, RDG and IGF‐1 (P < 0.01). Metabolic required calorie correlated positively with IGF‐1 (P < 0.01). Also, correlations of BW in POW, with BW and RDG in PRW were positive (P < 0.01). Growth in PRW would be influenced by maternal effects, while active self‐secretion of IGF‐1 in POW might contribute to POW growth. These factors suggested that to increase growth in PRW, maintaining enough maternal effect and IGF‐1 level in POW, was important for establishing better growth after weaning. 相似文献
3.
Astrid ARDIYANTI Yoshie OKI Yoshihito SUDA Keiichi SUZUKI Koichi CHIKUNI Yoshiaki OBARA Kazuo KATOH 《Animal Science Journal》2009,80(1):62-69
To investigate the effects of bovine growth hormone (bGH) gene polymorphism on carcass traits and fatty acid compositions in Japanese Black cattle caused by nucleotide substitution of CTG (allele A)/GTG (allele B) at codon 127 and of ACG (allele A and B)/ATG (allele C) at codon 172 of bGH, GH genotypes of 135 cattle were determined using allele specific-multiplex polymerase chain reaction (PCR). Allele A gave greater rib thickness and lower melting point of fat (MP) while allele B gave higher C18:1% ( P < 0.05). Allele C gave higher C18:1, monounsaturated fatty acid (MUFA), unsaturated fatty acid (USFA) percentages ( P < 0.05). It also gave lower saturated fatty acid (SFA) percentages, higher MUFA/SFA and USFA/SFA ratios, and lower MP ( P < 0.05). Interactions of sex and GH alleles were analyzed. In heifers, allele A gave higher carcass weight, daily carcass gain, rib eye area, rib thickness, subcutaneous fat thickness, and BMS while allele B gave greater rib eye area and rib thickness ( P < 0.05). Allele C gave higher C18:1 ( P < 0.01), MUFA ( P < 0.01), USFA percentages ( P < 0.05) and MUFA/SFA and USFA/SFA ratios ( P < 0.01), and lower C16:0 and SFA percentages ( P < 0.05) and MP ( P < 0.01). GH gene polymorphism affected carcass traits and fatty acid compositions although the effects were more pronounced in heifers. 相似文献
4.
Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
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Hideki KINOSHITA Shun IMOTO Yoshihito SUDA Mitsuharu ISHIDA Masamichi WATANABE Yasushi KAWAI Haruki KITAZAWA Koh MIURA Akira HORII Tadao SAITO 《Animal Science Journal》2013,84(2):150-158
Adhesion tests are complex, time‐consuming and expensive, while the most important criterion for a probiotic lactobacilli is the ability to adhere to the human intestine. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) activity using a microtiter plate screening method. GAPDH activities were detected in 21 out of 30 samples from 12 h cultures and in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. A statistically significant positive correlation was shown between GAPDH activity and adhesion using the BIACORE adhesion assay (P < 0.01). The new screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the significant positive correlation of GAPDH activity with adhesion of lactobacilli derived from the human intestine. 相似文献
7.
In this study, we examined the genetic diversity in three populations of the critically endangered annual, Bidens cernua, in Japan by using inter‐simple sequence repeat markers and compared our data with those from two common congeners: Bidens radiata var. pinnatifida and Bidens tripartita. In contrast to our expectations, the degree of genetic diversity at the species level was higher in B. cernua than in B. radiata var. pinnatifida or B. tripartita. At the population level, the degree of genetic diversity was highest in B. cernua. These results may be ascribed to the mating system and method of seedbank formation in B. cernua. An analysis of molecular variance revealed relatively high genetic differentiation among the populations of all three species. We concluded that the distribution width could not be an index of genetic variability in Bidens examined in this study. 相似文献
8.
Aomi SUDA Tomoyuki MAKINO Yuji MAEJIMA Ikuko AKAHANE Teruo HIGASHI 《Soil Science and Plant Nutrition》2009,55(5):623-626
We used sequential extraction to investigate changes in the amounts of six chemical forms of manganese, cobalt and cadmium in soil samples after chloroform fumigation. The six forms were designated as follows: exchangeable, dilute-acid-soluble, manganese-oxide-occluded, organically bound, iron-oxide-occluded and residual. For all three metals, the decreases in the amounts of manganese-oxide-occluded forms were equivalent to the sum of the increases in the amounts of exchangeable and dilute-acid-soluble forms. The amounts of the other three forms did not change significantly after fumigation. These results indicate that some of the cobalt and cadmium in the manganese oxides was converted into exchangeable and dilute-acid-soluble forms, which suggests that soil sterilization may increase the availability of these heavy metals to plants. 相似文献
9.
Hirotada TSUJII Masahiro NISHIOKA Ummay SALMA Abdul G. MIAH Takaaki MAKI Myung G. LEE 《Animal Science Journal》2007,78(5):535-540
This comparative study was to investigate the hypocholesterolemic effects of dietary Rhodopseudomonas palustris and Rhodobacter capsulatus on rats fed a high cholesterol diet. Thirty male Wister–Imamichi rats were assigned to three groups and fed on either a high cholesterol diet, or a high cholesterol diet supplemented with 2.0% R. palustris or R. capsulatus for 4 weeks. Compared to the control diet, both of the R. palustris and R. capsulatus supplemented diets significantly reduced the serum cholesterol, triglycerides, low‐density lipoprotein, very low density lipoprotein cholesterol and hepatic triglycerides, but increased hepatic cholesterol in rats. In addition, both of the R. palustris and R. capsulatus supplemented diets may reduce the risk of atherosclerosis, as the ratio of high density lipoprotein cholesterol to the total cholesterol was significantly higher than in the control group (P < 0.05). Both the R. palustris and R. capsulatus supplemented diets led to an increase in the serum palmitic acid, compared with the oleic acid and linoleic acid. No significant differences were postulated between the rats fed R. palustris and R. capsulatus supplemented diets during the 4 weeks of the experimental period. Thus, the results may suggest that both R. palustris and R. capsulatus can contribute significant health benefits and seems to be feasible to investigate in future research. 相似文献
10.
Hirotada TSUJII Yasushi KUWABARA Ummay SALMA Abdul Gaffar MIAH Miki NISHIMURA Takaaki MAKI 《Animal Science Journal》2008,79(4):460-465
The study was designed to investigate the effect of Rhodobacter capsulatus on serum and meat cholesterol, fatty acid composition in meat, as well as meat quality of finishing pigs. A total of 16 120-day-old Landrace female pigs of about 60 kg initial body weight were randomly assigned into two groups. The pigs were fed a supplemented diet with 0.04% dietary R. capsulatus or a control diet for 60 days. Total cholesterol and low density lipoprotein-cholesterol concentration in serum was significantly lowered ( P < 0.05) in the pigs fed the R. capsulatus supplemented diet compared to the control diet. Carcass weight, carcass length, shoulder fat, back fat and loin fat thickness, longissimus muscle (LM) area, and color score did not differ significantly between the pigs fed the R. capsulatus supplemented diet and control diet. Among the nutrient composition of LM meat, neutral fat and triglyceride concentration were significantly ( P < 0.05) reduced in the finishing pigs by dietary supplementation of R. capsulatus . The proportions of n-6 PUFA were higher ( P < 0.05) in the pigs fed the R. capsulatus supplemented diet than in the pigs fed the control diet. The supplementation of dietary R. capsulatus to finishing pig diet played important roles in reducing serum cholesterol and meat triglycerides, as well as in increasing polyunsaturated fatty acid content in LM meat. 相似文献