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DAVID GELA MARTIN FLAJŠHANS MARTIN KOCOUR MAREK RODINA OTOMAR LINHART 《Aquaculture International》2006,14(1-2):195-203
A basic assumption for carrying out the breeding work is to have well-organised broodstock management. Taking into account
the number of populations bred and the limited number of ponds available, it is impossible to avoid rearing several juvenile
or adult breeds in mixed stocks. Fish of individual breeds are group-marked with regular renewal of the freeze-branded mark
and broodstock adults should be marked individually. To minimise inbreeding or losses in genetic variation, at least 120 fish
per strain are reared and when the strain is restored, factorial crosses of at least 15 females and 25 males should be applied.
All activities are individually registered in the “Evidence 2003” data-recording software and data can be sorted by the origin
of the fish population, of the strain or of individual fish; reproductive and performance parameters are recorded individually
also. Further breeding operations with tench comprise the selection after over-wintering and rearing of fish before, during
and after the reproductive season. 相似文献
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Various procedures for artificial insemination in tench, Tinca tinca (L.) were re-examined with evaluation of fecundity of males and females among different tench strains. The objectives of
this study were to enhance fertilization and hatching rates through optimization of the activation solution, the insemination
process, the activation of gametes, and the elimination of eggs stickiness. Sperm for all experiments was collected directly
into immobilization solution of modified Kurokura solution containing 180 mM of NaCl and stored at 2 °C for 2.5–5 h prior
to the experiment. When dechlorinated tap water was used for activation a gamete ratio of 1150 spermatozoa per egg showed
the best significant fertilisation and hatching rates. Optimal ratio between eggs (weight in g) and activation solution (in
cm3) was 1:1. Different concentrations of activation solutions such as NaCl from 0 to 68 mM (0–136 mOsmol kg−1) without buffer statistically decreased fertilization and hatching rates. The activation solution containing 17 mM of NaCl,
10 mM Tris–HCl, pH 8 and 9 significantly increased fertilization and hatching rates compared to dechlorinated tap water of
pH 7 or activation solution containing 17 mM of NaCl, 10 mM Tris–HCl, pH 6 and 7. Adhesiveness of the eggs was successfully
removed by incubation in Alcalase and activity: 3.16 Anson units per cm3. 相似文献
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