用植株转化法将GUS基因导入桑树幼苗的研究   总被引：5，自引：2，他引：3  

陆小平  楼程富  王波  小岛峯雄 《蚕业科学》2004,30(2):129-132

为探讨简便易行的农杆菌介导的转化方法 ,用工程农杆菌A 110株 (LBA4 4 0 4 /pIG12 1 Hm)对桑幼苗子叶腋隙进行刺伤感染 ,感染部位长出的桑芽表型异常。取其基因组DNA进行分子生物学鉴定 ,结果显示 :以基因组DNA为模板 ,经PCR反应后得到特异性扩增片段用SalⅠ酶解 ,酶切产物与预计大小完全相符 ;用GUS基因作探针进行Southern杂交和用Km基因作探针对基因组DNA进行Southern杂交 ,均获得较强的杂交信号。  相似文献   

Distribution and abundance of resting cysts of the toxic Alexandrium spp. (Dinophyceae) in sediments of the western Seto Inland Sea, Japan   总被引：1，自引：0，他引：1  

MINEO  YAMAGUCHI  SHIGERU  ITAKURA  KEIZO  NAGASAKI  YUICHI  KOTANI 《Fisheries Science》2002,68(5):1012-1019

Sediment samples were collected from 135 stations in the western part of the Seto Inland Sea (Iyo Nada, Suo Nada, Beppu Bay, Tokuyama Bay, Hiroshima Bay, Aki Nada, Hiuchi Nada and Bingo Nada) to determine the horizontal distribution and abundance of resting cysts of Alexandrium spp. ( A. tamarense  +  A. catenella ). Enumeration of the cysts was performed using the primuline-staining direct count method. Cysts of Alexandrium spp. were rarely found in Iyo Nada, Suo Nada and Beppu Bay, but were widely distributed in Tokuyama Bay, Hiroshima Bay, Aki Nada, Hiuchi Nada and Bingo Nada. Cyst concentrations ranged from not detected (ND) to 14, ND to 17, ND to 4, 93 to 8137, 8 to 4454, ND to 6, ND to 18 and 4–29 cysts/cm³ wet sediment in Iyo Nada, Suo Nada, Beppu Bay, Tokuyama Bay, Hiroshima Bay, Aki Nada, Hiuchi Nada and Bingo Nada, respectively. The majority of cysts occurred in Tokuyama Bay and Hiroshima Bay, where higher densities were observed in the inner bay and along the coastal margin. Relatively higher cyst concentrations were observed at stations with a higher mud content. The abundance of Alexandrium spp. cysts in western Seto Inland Sea is lower than in the eastern Seto Inland Sea, except for Tokuyama Bay and Hiroshima Bay. However, because sporadic blooms of Alexandrium have been observed, continuing monitoring is necessary to prevent paralytic shellfish poisoning outbreaks in this area.  相似文献