排序方式： 共有7条查询结果，搜索用时 15 毫秒
1
1.
Abstract. The present study was performed to obtain further details on the immuno-modulating effects of oxytetracycline (OTC) in carp, Cyprinus carpio L. The immuno-logical study was extended by investigating the pharmacokinetic behaviour of OTC. Oxytetracycline, at a dosage of 60 mg/kg, was administered five times intra-peritoneally at 3-day intervals; the first injection was given 1 day before immunization. The kinetics of the primary anti-sheep red blood cell (SRBC) response, during a short-term OTC treatment revealed that the kinetics of the plaque-forming cell (PFC) response was not significantly affected by OTC nor its solvent. In contrast, the number of plaque-forming cells was significantly decreased. The anti-SRBC antibody production was delayed by 2–4 days in both experimental groups (OTC and solvent). However, within 12–14 days post-immunization, the same antibody levels were detected as in the control group. High OTC plasma levels (>50 μg/ml) were detected about 10 h after a single i. p. injection. A mean plasma elimination half-life (T1 /2 ) of 34·5 h was calculated. 相似文献
2.
Abstract. The present study was undertaken to identify female breeders, resistant or susceptible to disease, which might be used to obtain gynogenetically cloned carp lines differing in disease resistance. Experimentally induced erythrodermatitis was used as the disease model. Firstly, the effect of age on the resistance to bath challenge with atypical Aeromonas salmonicida was examined. These challenges indicated a shift from subacute to chronic infection with increasing age, as shown by a lower survival at 3 and 5 months (both 15%) compared to 10 months of age (60%). Then, to conserve and characterize breeder females, offspring of two females (nos 21 and 38), including F1, F2 and backcross (B1 and B2) progenies, were bath challenged at the age of 3 months. Comparison of the survival data showed a segregation into two groups of progenies: one resistant with nearly 100% survivors (W, F1 and B2), and one relatively susceptible group with 25% (R8 and F2) or 50% (B1) mortality. Analysis of inheritance indicated dominance of the resistant phenotype. Thus, the results identified two breeder females whose (gynogenetic) progeny might be expected to differ in resistance to bath challenge with atypical A. salmonicida. 相似文献
3.
D. EVENBERG P. de GRAAFF B. LUGTENBERG W. B. Van MUISWINKEL 《Journal of fish diseases》1988,11(4):337-350
Abstract The development and applicability of a dose-controlled experimental infection with atypical Aeromonas salmonicida in carp is described. The proliferation and clinical manifestations of experimentally induced carp erythrodermatitis mimicked a natural infection. An in-vivo assay was used to evaluate the lethal properties of cell-free culture supernatants and a simple serum-free growth medium was devised for maintaining the virulence of the challenge strain. Depending on the inoculation dose, a sublethal (chronic) to a lethal (acute) infection could be induced, and a dose-response relation was observed between A. salmonicida inoculum size and carp mortality. The dose-controlled experimental infection was used as a challenge test for laboratory evaluation of the efficacy of potential vaccine candidates. The vaccine candidates tested, a cell envelope preparation, purified lipopolysaccharide and purified A-layer (ACE) protein showed no protection or only a feeble one at the best, while formalinized whole cells showed a consistent but only moderate protection. In contrast, when concentrated, detoxified culture supernatant was used, the carp were protected against a subsequent lethal challenge. These observations indicate that immunity against A. salmonicida extracellular products is of prime importance. 相似文献
4.
G. HOUGHTON G. F. WIERGERTJES A. GROENEVELD W. B. VAN MUISWINKEL 《Journal of fish diseases》1991,14(3):333-341
Abstract. The degree of resistance to an atypical strain of Aeromonas salmonicida , the causative bacterium of carp erythrodermatitis, was examined in two strains of carp, Cyprinus carpio L.: a Polish line. R3, sixth generation of conventional inbreeding (full-sib matings); and a Hungarian line. R8, fifth generation of conventional inbreeding. Comparisons were made between and within the two strains. Results showed a significant difference ( P < 0·001) in the degree of resistance, with the Hungarian carp showing greater resistance than the Polish carp. Differences within each strain were also observed indicating a maternal influence on resistance. Two transferrin genotypes in three genetic combinations were identified (DD, DG, GG) but were not found to influence resistance. 相似文献
5.
Abstract. A bath challenge system was used to infect carp. Cyprinus carpio L., with Aeromonas salmonicida subsp. nova , the causative agent of carp cruthrodermatitis. Bath-challenged fish became infected with the bacterium exihibitinng typical signs of the disease, Carp that were sublethally bath exposed became infected and exhibited some skin lesions, but after one week, these quickly healed and the animals fully recovered from the infection, Naive fish that had not been previously exposed to the bacterium had mortalities of 100% when infected by the subcutaneous route and 40–60% by the bath route of infection. Carp that received sublethal infections were able to withstand subsequent lethal infection and recover regardless of the route of infection. Sublethally bath-exposed carp were protected from subsequently lethal challenges of A. salmonicida subsp. nova for at least 5 months. 相似文献
6.
7.
Abstract. The development of immunological memory was investigated in carp following direct immersion in Aeromonas hydrophila bacterin. A single bath did not induce an increase in serum antibody (Ab) levels. A second bath at 1, 3 or 8 months resulted in clear secondary Ab responses. The highest Ab levels were reached in the group boosted after 3 months. However, at 12 months a second bath gave no further significant response. When a booster was given at the same intervals by intramuscular injection, instead of immersion, the induced memory was not expressed as a clear secondary response. It is concluded that direct immersion in a bacterial antigen did induce immunological memory. The duration of this slowly developing memory is limited in time (less than 12 months) and was only demonstrated following a second immersion. This last feature suggests the existence of a local or mucosal activity during the immune response after bath vaccination. 相似文献
1