Ultrasound-Assisted Extraction for the Recovery of Carotenoids from Guava’s Pulp and Waste Powders     

da Silva Lima  Renan  Nunes  Itaciara Larroza  Block  Jane Mara 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):63-69

Plant Foods for Human Nutrition - In this work, lycopene- and β-carotene-rich extracts were obtained from guava’s pulp and waste powders using maceration (ME), ultrasonic bath (BUAE,...  相似文献   

Optimizing injection time of GFP plasmid into sheep zygote     

Mohammadreza Ebrahimi  Laura Mara  Bernardo Chessa  Fabrizio Chessa  Abbas Parham  Maria Dattena 《Reproduction in domestic animals》2021,56(3):467-475

Microinjection of exogenous DNA into the cytoplasm of matured oocytes or zygotes is a promising technique to generate transgenic animals. However, the data about the microinjection time and procedure in sheep are limited and have not treated in detail. To obtain more in-depth information, the Sarda sheep oocytes from abattoir-derived ovaries were subjected to IVM and IVF. Then, the GFP plasmid as a reporter gene was injected into the cytoplasm of MII oocytes (n: 95) and zygotes at different post-insemination intervals (6–8 hpi, n: 120; 8–10 hpi, n: 122; 10–12 hpi, n: 110 and 12–14 hpi, n: 96). There were no significant differences in the cleavage rates between the groups. However, blastocyst rate of injected zygotes at all-time intervals was significantly lower than injected MII oocytes and control group (p < 0.05). Interestingly, the proportion of GFP-positive embryos was higher at 8–10 hpi compared with other injected groups (4 % versus 0 %, p  < 0.01). Among these, the proportion of mosaic embryos was high and two of those embryos developed to the blastocyst stage. In conclusion, we settled on the cytoplasmic microinjection of GFP plasmid at 8–10 hpi as an optimized time point for the production of transgenic sheep and subsequent experiments.  相似文献   

Intake,digestibility and performance of lambs fed diets containing peach palm meal     

Alana Batista dos Santos  Mara Lúcia Albuquerque Pereira  Herymá Giovane de Oliveira Silva  Gleidson Giordano Pinto de Carvalho  Taiala Cristina de Jesus Pereira  Leandro Sampaio Oliveira Ribeiro  José Augusto Gomes Azevêdo  Maria das Graças Conceição Parada Costa Silva  Larisse Borges Sousa  Leandro Borges Sousa  Daiane de Oliveira Alencar 《Tropical animal health and production》2016,48(3):509-515

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Effect of supplementation of beef cattle with different protein levels and degradation rates during transition from the dry to rainy season     

Rodolfo Maciel Fernandes  Chafic Mustafé de Almeida  Bruna Caldas Carvalho  João Alexandrino Alves Neto  Verônica Aparecida Costa Mota  Flávio Dutra de Resende  Gustavo Rezende Siqueira 《Tropical animal health and production》2016,48(1):95-101

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The use of intralipid emulsion therapy to treat status epilepticus in a guinea pig (Cavia porcellus) secondary to severe ivermectin toxicity     

Jennifer J. Ebel DVM  Kristi Gannon DVM  DACVECC  Mara Kraenzlin VMD  DACVECC 《Journal of Veterinary Emergency and Critical Care》2023,33(1):107-111

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Successful management of minoxidil 5% toxicosis in 2 cats from the same household     

Tyler E. Johnson DVM  Meghan E. Fick DVM  MS  DACVECC  Jenica L. Haraschak MS  DVM  DACVECC  Mara E. Vernier DVM  Saki Kadotani DVM  MS  DACVIM 《Journal of Veterinary Emergency and Critical Care》2023,33(4):454-459

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<Emphasis Type="Italic">In vitro</Emphasis> screening for sunflower (<Emphasis Type="Italic">Helianthus annuus</Emphasis>L.) resistant calli to <Emphasis Type="Italic">Diaporthe helianthi</Emphasis> fungal culture filtrate     

Mara Quaglia  Antonio Zazzerini 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(4):393-400

Diaporthe helianthi the causal agent of sunflower (Helianthus annuus) stem canker, causes significant reductions in yield and oil content in most sunflower-growing areas. With the aim of enhancing host resistance, we selected in vitro sunflower calli against culture filtrates of two pathogen isolates (7/96 and 101/96). This technique may be an effective and rapid tool to discriminate the most virulent D. helianthi isolate and to screen for host resistance in the early stage of a breeding programme. Further investigation on the mechanisms involved in defence pathways showed no induction of salicylic acid and pathogenesis-related proteins in calli, indicating that the host resistance is not associated with Systemic Acquired Resistance but probably other biochemical mechanisms.  相似文献   

Molecular evidence of Leishmania spp. in spider monkeys (Ateles geoffroyi) from The Tuxtlas Biosphere Reserve,Veracruz, Mexico     

Pérez-Brígido  Carlos D.  Romero-Salas  Dora  Pardío-Sedas  Violeta T.  Cruz-Romero  Anabel  González-Hernández  Milagros  Delprá-Cachulo  Joyce Mara  Ascencio  Mariano  Florin-Christensen  Mónica  Schnittger  Leonhard  Rodríguez  Anabel E. 《Veterinary research communications》2022,46(1):295-302

Veterinary Research Communications - The black-handed spider monkey (Ateles geoffroyi) is a platyrrhine primate distributed in southern Mexico, Central America, and part of South America. Two...  相似文献   

Canine circovirus and Canine adenovirus type 1 and 2 in dogs with parvoviral enteritis     

Balboni  Andrea  Terrusi  Alessia  Urbani  Lorenza  Troia  Roberta  Stefanelli  Silvia A. M.  Giunti  Massimo  Battilani  Mara 《Veterinary research communications》2022,46(1):223-232

Veterinary Research Communications - Canine parvovirus type 2 (CPV-2) is one of the most relevant pathogens associated with enteritis in dogs and is frequently reported in association with the...  相似文献   

Vegetation restoration is associated with increasing forest width     

Londe  Vinícius  Messias  Maria Cristina Teixeira Braga  de Sousa  Hildeberto Caldas 《New Forests》2021,52(1):129-144

New Forests - Monitoring and assessment reveal important information about restoration areas and can also be useful for understanding ecological processes such as succession and species-area...  相似文献