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Masahiko Awaji Toshie Matsumoto Daisuke Ojima Shunsuke Inoue Michio Suzuki Masaei Kanematsu 《Fisheries Science》2018,84(3):535-551
For recovery of the declining population of pen shells in the wild, the production of pen shell juveniles for transplantation or aquaculture is underway in Japan. For more stable juvenile production, artificial fertilization methods for pen shells are needed, but methods to induce oocyte maturation (meiosis resumption) used in other bivalves, which make oocytes fertilizable, were ineffective for pen shells. Here, we report evidence showing that retinoic acid (RA) has strong activity in inducing oocyte maturation and activating sperm motility in pen shells. Treatment of fully developed oocytes with 1.0 μM all-trans-RA (at-RA) induced germinal vesicle breakdown, a typical morphological sign of oocyte maturation, but 1.0 μM at-retinol and at-retinal, 2 mM ammonia, and 1.0 μM serotonin were ineffective. Treatment with at-RA for 30 min was sufficient for oocyte maturation and was more potent than its isomers, 9-cis- and 13-cis-RA. Parallel results were obtained for sperm motility activation. Oocyte responsiveness to at-RA increased during the final stage of ovary development. Artificial fertilization was successful only with the oocytes treated with at-RA, and fertilized eggs developed to D-shaped (veliger) larvae without apparent morphological abnormalities. These results indicate the possible application of RA for the artificial fertilization of pen shells. 相似文献
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Uchida Motoharu Miyoshi Tatsuo Kanematsu Masaei Kobayashi Yutaka 《Fisheries Science》2020,86(3):519-530
Fisheries Science - The suitability of fermented products of Ulva fronds as diets for juvenile asari clams was tested. Ulva fronds were transformed by fermentation into a single-cell product (Ulva... 相似文献
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SUMMARY: Adenocarcinomatous growths comprising glandular cavities surrounded by cuboidal to low columnar cells and a large amount of mucus produced by the mucous cells, were observed in the the gastric lymph nodes of a harbor seal Phoca largha. The seal was stranded on the coast of Toyama Bay, Sea of Japan, on 2 March, 1998. Necroscopic examination also showed the animal had significant signet ring cell carcinoma in the stomach, as has been reported elsewhere. The adenocarcinoma may have developed from metastatic cells arising in the gastric carcinoma. 相似文献
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Kurihara Takeo Hashimoto Kazumasa Nakano Shoji Matsuyama Yukihiko Ito Atsushi Kanematsu Masaei Okamura Kazumaro 《International Aquatic Research》2018,10(4):349-359
International Aquatic Research - In the inner area of the Sea of Ariake, rearing experiments were conducted to examine how the survival rates of juveniles of three bivalve species (Anadara... 相似文献
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Kurihara Takeo Nakano Shoji Matsuyama Yukihiko Hashimoto Kazumasa Yamada Katsumasa Ito Atsushi Kanematsu Masaei 《International Aquatic Research》2018,10(1):1-11
International Aquatic Research - Survival time of juvenile pen shell Atrina pectinata (shell length ≈ 34 mm) exposed to hyposaline water without sediments was... 相似文献
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