Effect of intramammary injection of rboGM-CSF on milk levels of chemiluminescence activity, somatic cell count, and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis          下载免费PDF全文

Hideyuki Takahashi  Masaharu Odai  Kenji Mitani  Shigeki Inumaru  Shozo Arai  Rieko Horino    Yuichi Yokomizo 《Canadian journal of veterinary research》2004,68(3):182-187

The effect of intramammary injection of recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF, 400 microg/10 mL) on quarter milk levels of chemiluminescence (CL) activity, and somatic cell count (SCC) and shedding pattern of Staphylococcus aureus was investigated. Ten Holstein cows, naturally infected with S. aureus were used, with either early-stage or late-stage subclinical mastitis. Injection of rboGM-CSF caused a remarkable increase in milk CL activity with a peak at 6 h after the cytokine injection in the early- and late-stage groups. In the early-stage group, milk SCC stayed around preinjection level at 6 h, rose significantly on days 1 and 2, and was followed by a smooth and significant decline to an under preinjection level (below 200 000 cells/mL) on day 7 postinjection. Alternatively, in the late-stage group, milk SCC rose significantly at 6 h after the cytokine injection and maintained high levels thereafter. The milk S. aureus count decreased drastically by the cytokine injection in the early-stage group. The bacterial count was moderately decreased in the late-stage group, but increased back to preinoculation levels on day 7 after the cytokine injection. The results suggest that the rboGM-CSF has a potential as a therapeutic agent for S. aureus infection causing subclinical mastitis of dairy cows, if the cytokine is applied at the initial stage of infection.  相似文献   

A morphological analysis of the skull size and shape of Kerivoulinae (Chiroptera: Vespertilionidae) from Vietnam     

Son Truong NGUYEN  Masaharu MOTOKAWA  Tatsuo OSHIDA  Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):187-198

Southeast Asia is a region of high biodiversity, containing species of plants and animals that are yet to be discovered. In this region, bats of the subfamily Kerivoulinae are widespread and diverse with six species recorded in Vietnam. However, the taxonomy of the Kerivoulinae in Asia is complicated. In our study, we used diagnostic characters and multivariate analysis to determine morphological differences between the genera Kerivoula and Phoniscus. We showed that the two genera are distinguishable by the size of second upper incisors, the shape of skull, nasal sinus, canines, second upper and lower premolars. In addition, the two genera can be osteometrically separated by measurements of the braincase height, interorbital width and shape of anterior palatal emargination. Our data clearly revealed the morphological variations in the skull shape of Kerivoula hardwickii in Vietnam. This suggests a possible separation into three morphotypes, representing cryptic species supported by statistical differences with wide variation in skull shape, size and teeth. These results demonstrated Kerivoula hardwickii can be separated three subspecies, and the result will serve as the basis for the future assessment and classification of this group in Southeast Asia.  相似文献   

A dog with myelodysplastic syndrome: chronic myelomonocytic leukemia     

Hiraoka H  Hisasue M  Nagashima N  Miyama T  Tanimoto T  Watanabe M  Itamoto K  Mizuno T  Inokuma H  Okuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):665-668

An eleven-year-old female pug was referred to Yamaguchi University Animal Hospital for evaluation of anemia and thrombocytopenia. The cytological examination of the peripheral blood showed some giant monocytic lineage blast cells. A few granulocytes and platelets had dysplastic features. On day 7, in addition to increasing the monocytic lineage cells, the dysplastic features of the blood had also increased compared to the initial examination. We performed bone marrow aspiration upon her death. The bone marrow revealed dysplastic features in all three hematopoietic cell lines, and an increase in the monocytic cell line. Based on the features of the bone marrow and the peripheral blood, this case was confirmed to be myelodysplastic syndrome--Chronic myelomonocytic leukaemia (MDS-CMML).  相似文献   

Evaluation of high sensitive DNA probe for the detection of Theileria sergenti infection in cattle.   总被引：2，自引：0，他引：2  

A Hirano  R Kirisawa  T Matsuba  R Komatsu  M Tanaka  K Takahashi  Y Kawakami  M Onuma 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(5):933-935

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Abnormal structural luteolysis in ovaries of the senescence accelerated mouse (SAM): expression of Fas ligand/Fas-mediated apoptosis signaling molecules in luteal cells     

Kiso M  Manabe N  Komatsu K  Shimabe M  Miyamoto H 《The Journal of reproduction and development》2003,49(6):457-463

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Clinical response of ovarian cysts in dairy cows after PRID treatment     

Zulu VC  Nakao T  Yamada K  Moriyoshi M  Nakada K  Sawamukai Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):57-62

We investigated the therapeutic effects of a progesterone releasing intravaginal device (PRID) on cystic ovarian disease (COD) and reproduction performance of cows. The possible influence of PRID on metabolic and/or health status was also examined. A total of 40 Holstein-Friesian cattle, with ovarian cystic structures, > or =2.5 cm in diameter, persisting for more than 7-14 days, without a corpus luteum (CL) were used for the study. PRID or placebos were inserted into the vagina for 12 days. Five animals lost the intravaginal device before removal and one was culled. Based on plasma progesterone concentration on the day of treatment, 20 (17 PRID and 3 placebos) of the remaining 34 cows had follicular cysts (progesterone < or =1 ng/ml) and 14 (10 PRID and 4 placebos) had luteal cysts (progesterone >1 ng/m l). Fourteen (82%) of the PRID-treated follicular cystic cows responded with formation of a CL within 14 days after treatment, and an overall conception rate of 53.8%. Likewise, 70% of the treated luteal cystic cows responded with CL formation and 71.4% conception rate. No significant differences were observed in hematocrit (Ht), white blood cell count and serum levels of glucose, blood urea nitrogen, aspartate aminotransferase, and alanine aminotransferase, between the day of PRID insertion and removal, in animals with follicular and luteal cysts. PRID treatment resulted in ovulation 2-4 days later and formation of a CL in cows that recovered.  相似文献   

Central and peripheral concentrations of tumor necrosis factor-alpha in Chinese Meishan pigs stimulated with lipopolysaccharide     

Sakumoto R  Kasuya E  Komatsu T  Akita T 《Journal of animal science》2003,81(5):1274-1280

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Classification of abomasal displacement in cows according to histopathology of the liver and clinical chemistry     

Komatsu Y  Itoh N  Taniyama H  Kitazawa T  Yokota H  Koiwa M  Ohtsuka H  Terasaki N  Maeno K  Mizoguchi M  Takeuchi Y  Tanigawa M  Nakamura T  Watanabe H  Matsuguchi Y  Kukino T  Honma A 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(9):482-486

Histopathological features of livers and blood chemical values in cows with abomasal displacement were investigated. Liver biopsy samples were collected during redressment operations in 92 cows with abomasal displacement, and the samples were stained with haematoxylin and eosin or periodic acid Schiff (PAS). Blood was collected for chemical tests. Livers were histopathologically divided into the following four types: normal histology cases (21%), fatty degeneration cases (36%), cloudy swelling cases (19%) and fatty degeneration cases with cloudy swelling (24%). The number of PAS-positive samples was significantly higher in the normal histology group and significantly lower in the severe fatty degeneration group and severe cloudy swelling group. Cows with fatty degeneration had significantly higher levels of serum 3-hydroxybutyric acid, non-esterified fatty acid and aspartate aminotransferase than did those with cloudy swelling or normal histology. The results indicate that the morbid conditions of cows with abomasal displacement can be classified into four types.  相似文献   

In vitro detection of equine arteritis virus from seminal plasma for identification of carrier stallions   总被引：1，自引：0，他引：1  

Fukunaga Y  Wada R  Sugita S  Fujita Y  Nambo Y  Imagawa H  Kanemaru T  Kamada M  Komatsu N  Akashi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(6):643-646

Equine arteritis virus (EAV) was readily isolated in RK-13 cell monolayers by plaque assay from seminal plasma of experimental carrier stallions when they contained high titers of virus regardless of the presence of non-viral cytotoxicity in the seminal plasma. The cytotoxicity interfered with virus isolation from seminal plasma which contained virus at titers less than 10 PFU/ml. However, it was possible to detect the virus in seminal plasma pretreated with PEG (#6000). EAV was consistently identified by RT-PCR from crude seminal plasma which contained virus at titers of more than 10(2.7) PFU/ml. In vitro detection of EAV by virus isolation supplemented with RT-PCR using seminal plasma was proved to be an effective alternative to the standard test mating as a diagnostic method for carrier stallions.  相似文献   

Appearance of race Pfs:5 of spinach downy mildew fungus, Peronospora farinosa f. sp. spinaciae, in Japan     

Mamoru Satou  Kazufumi Nishi  Masaharu Kubota  Michiko Fukami  Hideaki Tsuji  Kees van Ettekoven 《Journal of General Plant Pathology》2006,72(3):193-194

The races for the causal agent of spinach downy mildew Peronospora farinosa f. sp. spinaciae were identified by inoculation of race-differential cultivars. One isolate was identified as Pfs:5s and the others belonged to a new race. This is the first report of race Pfs:5 and another new race in Japan.  相似文献