Effect of dietary vitamins C and E fortification on lipid metabolism in red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli     

HONG  JI  Ahmad Daud  OM  Takao  YOSHIMATSU  Masahiro  HAYASHI  Tetsuya  UMINO  Heisuke  NAKAGAWA  Masaya  ASANO  Atsushi  NAKAGAWA 《Fisheries Science》2003,69(5):1001-1009

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Development and characterization of monoclonal antibodies against canine trypsin     

Takaki Waritani  Yoko Okuno  Yoshinori Ashida  Ryo Tsuchiya  Kosaku Kobayashi  Takatsugu Yamada   《Veterinary immunology and immunopathology》2001,80(3-4):333-338

Canine cationic trypsin was purified by salting-out, gel filtration and affinity chromatography. Purity was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight was ca. 28 kDa by SDS-PAGE.

Thirty hybridomas were obtained which produced mAb to canine cationic trypsin by the cell fusion technique. Twenty-two of these recognized cationic trypsin only, while eight hybridomas recognized both cationic and anionic trypsin. Several of the anti-canine cationic trypsin mAb were purified by salting-out and DEAE ion-change chromatography using ascites fluid of immunized BALB/c mice. The mAb proved to have very high specificity to canine cationic trypsin as shown by immunoblotting and it may be possible to use them to develop clinical assays.  相似文献   

Surgery of hepatic cysts in a cat.     

M Washizu  K Kobayashi  K Misaka  T Hayashi  G Kinoshita  M Kondo  S Aoki  H Orima  T Washizu 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):1051-1053

A 2-year-old female Japanese domestic cat weighing 3.6 kg was presented due to marked abdominal distention and was diagnosed as hepatic cyst. The bile acid concentration of the hepatic cyst was as low as the serum bile acid concentration. The membrane of the cysts was excised from the edge of the compressed hepatic tissue and no other surgical manipulation was performed. This surgical procedure is simple and quick compared to hepatic lobectomy, however, it is necessary to evaluate the fluid of the cyst before performing this procedure.  相似文献   

Homolog of FlhDC, a master regulator for flagellum synthesis: required for pathogenicity in Erwinia carotovora subsp. carotovora     

Hiroyuki Matsumoto  Masahiro Umehara  Hironobu Muroi  Yoshimasa Yoshitake  Shinji Tsuyumu 《Journal of General Plant Pathology》2003,69(3):189-193

 Erwinia carotovora subsp. carotovora (Ecc) is a causal agent of soft-rot diseases in a wide variety of plants. Here, we have isolated nonmotile mutants in Ecc by in vivo insertional mutagenesis using a transposon Tn5. The sequence disrupted by the Tn5 insertion in YMU1 and YMU5 mutants was highly homologous to that of flhC and flhD genes, respectively. They are involved in the initiation of the expression of flagellum-related genes in many gram-negative bacteria such as Escherichia coli and Salmonella. With electron microscopy, the flhC and the flhD homolog mutants were shown to be aflagellate. Furthermore, the virulence of these mutants was greatly reduced in Chinese cabbage and potato compared to that of the parental strain. These results suggest that flagellar formation is required for the pathogenicity of Ecc. Received: November 5, 2002 / Accepted: December 2, 2002 Acknowledgments This research was supported in part by Grant-in-Aid (12052210) and by a grant from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (13073).  相似文献   

Including an additional systematic environmental effect within a generation in an evaluation model improves accuracy of prediction of breeding values in a closed herd of pigs     

Masahiro SATOH 《Animal Science Journal》2004,75(2):97-102

The present study evaluated the advantage of mixed‐model techniques over a selection index under different magnitudes of an additional systematic environmental effect (ASEE) in terms of accuracy of prediction and expected genetic gain. The data attempted to simulate a closed herd in a pig breeding program. The base population (G₀) consisted of 10 males and 50 females. Six generations (G₀ to G₅) were selected by using a selection index of three traits without overlapping. Additional systematic environmental constants with four levels in a generation were assigned from a uniform distribution at different ranges. Breeding values of animals in the last generation (G₅) were estimated on the basis of an index of individual phenotype (SI‐U), SI‐U adjusted for ASEE using a least‐squares mean (SI‐A), best linear unbiased prediction using an animal model excluding ASEE (AM‐E), and an animal model including ASEE (AM‐I). Accuracy of prediction and expected genetic gain were larger by the animal model than by the selection index, even if heritability of the traits selected was high and ASEE was set to zero. When ASEE was zero, the accuracy of prediction and expected genetic gain given by SI‐U and AM‐I were similar to those given by SI‐A and AM‐E, respectively. However, the differences in accuracy and expected gain between SI‐U and AI‐A and between AM‐I and AM‐E increased as the range of ASEE increased. It was concluded that selection based on an animal model was more effective than index selection, even if the herd environment was uniform and traits with high heritability were selected, and that it should be always included in an evaluation model, however slight any systematic environmental effect may be in a closed herd.  相似文献   

Improving management for higher reproduction accelerates genetic improvement in closed herd of swine     

Masahiro SATOH 《Animal Science Journal》2004,75(6):499-502

The present study compared responses to selection at different conception rates and litter sizes at weaning in a simulated closed herd in a swine breeding program. The base population consisted of 10 males and 50 females, and 10 generations of selection was practiced by using individual phenotype or best linear unbiased prediction of breeding values for a trait with heritability (h²) of either 0.2 or 0.5. The probability of conception in a single mating was assumed to be 0.8, 0.9 or 1.0. Litter size at weaning was sampled randomly from a normal distribution with mean 8, 10 or 12 and variance 8.1225. Genetic response increased by approximately 6% for h² = 0.2 and approximately 5% for h² = 0.5 at generation 10 when conception rate was increased from 0.8 to 1.0. However, litter size at weaning did not affect response to selection. In conclusion, improving conception rate by environmental management increases genetic response indirectly in a breeding program of a closed swine herd.  相似文献   

Laboratory diagnosis of canine GM2-gangliosidosis using blood and cerebrospinal fluid.     

Osamu Yamato  Hiroyuki Satoh  Naoaki Matsuki  Kenichiro Ono  Masahiro Yamasaki  Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(1):39-44

In the present study, laboratory techniques were used to diagnose canine GM2-gangliosidosis using blood and cerebrospinal fluid (CSF) that can be collected noninvasively from living individuals. Lysosomal acid beta-hexosaminidase (Hex) was measured spectrofluorometrically using 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide and 4-methylumbelliferyl 7-(6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside) as substrates. Main isoenzymes A and B of Hex in leukocytes were also analyzed using cellulose acetate membrane electrophoresis. GM2-ganglioside in CSF was detected and determined quantitatively by using thin-layer chromatography/enzyme-immunostaining method with anti-GM2-ganglioside antibody. In normal dogs, Hex activities could be determined in leukocytes, serum, and CSF and the total activities were markedly reduced in all the enzyme sources in a dog with Sandhoff disease. Electrophoresis of a leukocyte lysate from a normal dog showed that the Hex A and Hex B were not separated distinctively with formation of a broad band, whereas there were no bands in electrophoresis of a lysate from a dog with Sandhoff disease, showing a deficiency in the total enzyme activity. GM2-ganglioside could be detected and determined quantitatively in as little as 100 microl of canine CSE GM2-ganglioside in CSF in a dog with Sandhoff disease increased to 46 times the normal level. In conclusion, the methods in the present study are useful for diagnosis of canine GM2-gangliosidosis. These techniques enable definitive and early diagnosis of canine GM2-gangliosidosis even if tissues and organs cannot be obtained.  相似文献   

Mitochondrial DNA analysis of Nepalese domestic dwarf cattle Lulu*     

Kumiko TAKEDA  Masahiro SATOH  Shreeram P. NEOPANE  Bahadur S. KUWAR  Hari D. JOSHI  Nanda P. SHRESTHA  Hiroshi FUJISE  Mariko TASAI  Takahiro TAGAMI  Hirofumi HANADA 《Animal Science Journal》2004,75(2):103-110

Dwarf Lulu cattle, the only Bos Taurus type of cattle in Nepal, are raised under severe environments in the mountainous zone of that country. In the present study, the body measurement traits, cytogenetic and molecular genetic characteristics of the Lulu cattle are investigated. Blood samples were collected from 31 animals in four villages (altitudes 2590–3550 m) in the southern part of Mustang. The Lulu cattle had a normal karyotype with 2n = 60, XY or XX. Only one male examined had a large submetacentric X‐chromosome and a small submetacentric taurine type Y‐chromosome. The mitochodrial DNA (mtDNA) genotypes were analyzed by PCR mediated restriction fragment length polymorphisms, displacement (D)‐loop region PCR mediated single strand conformation polymorphisms, and D‐loop region sequences. Many base substitutions were found in the D‐loop region, suggesting that the Lulu cattle originated from at least 10 maternal lines. Three types of mtDNA from these cattle were found, the Bos taurus type (n = 23), the Bos indicus type (n = 6), and the Bos grunniens type (n = 2). In the village at the lowest altitude, four of the five cows were of the Bos indicus type. These results indicated that mtDNA types of the Lulu cattle mostly belong to Bos taurus, but have been hybridized with Bos indicus cattle in lower‐elevation regions in their maternal lineage.  相似文献   

Effect of dasatinib in a xenograft mouse model of canine histiocytic sarcoma and in vitro expression status of its potential target EPHA2          下载免费PDF全文

K. Ito  R. Miyamoto  H. Tani  S. Kurita  M. Kobayashi  K. Tamura  M. Bonkobara 《Journal of veterinary pharmacology and therapeutics》2018,41(1):e45-e48

Canine histiocytic sarcoma (HS) is an aggressive and highly metastatic tumor. Previously, the kinase inhibitor dasatinib was shown to have potent growth inhibitory activity against HS cells in vitro, possibly via targeting the EPHA2 receptor. Here, the in vivo effect of dasatinib in HS cells was investigated using a xenograft mouse model. Moreover, the expression status of EPHA2 was examined in six HS cell lines, ranging from insensitive to highly sensitive to dasatinib. In the HS xenograft mouse model, dasatinib significantly suppressed tumor growth, as illustrated by a decrease in mitotic and Ki67 indices and an increase in apoptotic index in tumor tissues. On Western blot analysis, EPHA2 was only weakly detected in all HS cell lines, regardless of sensitivity to dasatinib. Dasatinib likely results in the inhibition of xenograft tumor growth via a mechanism other than targeting EPHA2. The findings of this study suggest that dasatinib is a targeted therapy drug worthy of further exploration for the treatment of canine HS.  相似文献   

A survey of antimicrobial resistance in Escherichia coli isolated from wild sika deer (Cervus nippon) in Japan     

Yukino TAMAMURA-ANDOH  Nobuyuki TANAKA  Keisuke SATO  Yoshino MIZUNO  Nobuo ARAI  Ayako WATANABE-YANAI  Masato AKIBA  Masahiro KUSUMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):754

We examined the antimicrobial susceptibility of 848 Escherichia coli isolates from 237 feces samples of wild sika deer (Cervus nippon) captured between 2016 and 2019 in 39 of the 47 prefectures of Japan. Five of the 237 wild sika deer (2.1%) carried E. coli with resistance to at least one antimicrobial, and all the resistant isolates showed resistance to tetracycline. The resistant isolates contained antimicrobial resistance genes that were similar to those in E. coli derived from humans and farm animals. Although wild sika deer are not currently likely to be a source for the transmission of antimicrobial resistance in Japan, they can potentially mediate antimicrobial resistance spread by coming into contact with humans, animals, and their surroundings.  相似文献