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1.
2.
Carotenoids are not only important to the plants themselves but also are beneficial to human health. Since citrus fruit is a good source of carotenoids for the human diet, it is important to study carotenoid profiles and the accumulation mechanism in citrus fruit. Thus, in the present paper, we describe the diversity in the carotenoid profiles of fruit among citrus genotypes. In regard to carotenoids, such as β-cryptoxanthin, violaxanthin, lycopene, and β-citraurin, the relationship between the carotenoid profile and the expression of carotenoid-biosynthetic genes is discussed. Finally, recent results of quantitative trait locus (QTL) analyses of carotenoid contents and expression levels of carotenoid-biosynthetic genes in citrus fruit are shown. 相似文献
3.
The mammalian uterus changes dramatically during the estrous cycle, pregnancy, and involution post partum. Dynamic changes in the uterine endometrium are a type of homeostasis and proceed with proliferation and exclusion of cells. Homeostasis of the uterus is closely related to apoptosis involving various hormones and cytokines. The objective of the present study was to determine the morphological features and occurrence of apoptosis in the porcine endometrium during the estrous cycle, early pregnancy, and post partum. Cyclic changes in the morphology of the surface epithelium were observed during the estrous cycle. The heights of surface epithelia were significantly high on day 4 of the estrous cycle and the early pregnancy. The heights of the surface epithelium remained low from days 1 to 31 post partum. We then used terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end-labeling (TUNEL) of the 3'-terminal of fragmented DNA, which is effective for detection of apoptosis in various tissues. We found that apoptosis in the porcine endometrium contributed to homeostasis of the endometrium during the estrous cycle through control of cell proliferation and exclusion. Conversely, apoptosis on days 4 and 8 of gestation before the implantation window depended on the plasma estrogen and progesterone levels; however, suppressive homeostasis of apoptosis occurred at the time of implantation on days 15, 18 and 21 of gestation. Our study is the first to demonstrate apoptotic cell death in the porcine endometrium directly by TUNEL method. The results strongly suggest that uterine homeostasis is mainly controlled by apoptosis during the estrous cycle and early pregnancy. 相似文献
4.
Toshihiro NADE Kazuhisa FUJITA Masashi FUJII Masaya YOSHIDA Teikichi HARYU Satsuki MISUMI Toshiaki OKUMURA 《Animal Science Journal》2005,76(5):513-517
An X‐ray computed tomography (CT) system for live standing cattle was developed for studying the meat yield, carcass composition and so on. The gantry contained three X‐ray tubes and detectors that corresponded to each X‐ray tube. The system was able to operate while the animal remained standing. The scan area had a diameter of 900 mm. The Musculus longissimus and Musculus trapezius areas, and the back fat thickness in the CT image were evaluated and compared to the actual cross‐section of the carcass using eight cattle. The differences among the muscles, and the subcutaneous and intermuscular fat were easily recognized. The correlation coefficient between the CT image and the actual carcass photograph of the M. longissimus area and the back fat thickness was high (r = 0.84, r = 0.93, P < 0.01). The present study demonstrated that muscle, fat and bone can be clearly imaged from a live standing animal using X‐ray equipment. 相似文献
5.
Isao Sawaguchi Shunji Shimizu Kouichi Ichihara Katsumi Toyokawa Osamu Ohkawabata 《Journal of Forest Research》1997,2(1):15-19
The growing condition of afforestation trees and damage to lower-story trees caused by felling and hauling of upper-story
trees in a multi-storied forest in Peninsular Malaysia was surveyed. Seventy months have passed since the planting ofAcacia mangium upperstory trees, which have grown 233 m3/ha in volume. The growth ofShorea leprosula thirty-five months after planting of lower-story trees have reached 5.54 m in height. However, as the ratio of height to
basal diameter exceeds 100:1, trees are tapering to a considerable degree. A backhoe was installed in order to fell the upper-story
trees. From the results of logging, the damage ratio estimation of the lower-story trees was simulated by using the Monte
Carlo method. The damage ratio differed considerably depending on planting patterns, height of upper-story trees and felling
skills. The results of this survey proved that the felling and hauling of upper-story trees would be technically feasible
in the future, except when one row upper-story trees and one row lower-story trees method were employed. 相似文献
6.
Kouichi Ichihara Tosimi Tanaka Isao Sawaguchi Shuji Umeda Katsumi Toyokawa 《Journal of Forest Research》1996,1(1):45-49
This study examines a method for designing an optimum profile of a forest road in a short matter of time. In this method,
a genetic algorithm (GA) searches for points where the gradient changes and dynamic programing (DP) designs the longitudinal
slope. This method can be applied to the ground heights of an actual forest road. The evaluation function of the DP assesses
the total expenditure. The inverse of this function is the fitness function. Crossover and mutation are operators of an evolution
simulation of a GA. They are each repeated 20 times and 420 profiles are designed and assessed, all within about 10 min. A
comparison of the profile designed by the GA with that of an actual forest road shows only one section where the formation
level differed substantially. Overall, both profiles seem to show a high degree of correspondence. From this result, we believe
that the profile designed by the GA is best, or the one of the best. A conventional method takes about 65 h to calculate all
changing points of gradients. However, by using the GA, the calculation time was reduced to about 1/389 that of the coventional
method, showing that the method which uses the GA through the profile design is very useful. 相似文献
7.
Satoshi KAMBAYASHI Masaya IGASE Kosuke KOBAYASHI Ayana KIMURA Takako SHIMOKAWA MIYAMA Kenji BABA Shunsuke NOGUCHI Takuya MIZUNO Masaru OKUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1405-1412
Hypoxic conditions in various cancers are believed to relate with their
malignancy, and hypoxia inducible factor-1α (HIF-1α) has been shown to be a major
regulator of the response to low oxygen. In this study, we examined HIF-1α expression in
canine lymphoma using cell lines and clinical samples and found that these cells expressed
HIF-1α. Moreover, the HIF-1α inhibitors, echinomycin, YC-1 and 2-methoxyestradiol,
suppressed the proliferation of canine lymphoma cell lines. In a xenograft model using
NOD/scid mice, echinomycin treatment resulted in a dose-dependent regression of the tumor.
Our results suggest that HIF-1α contributes to the proliferation and/or survival of canine
lymphoma cells. Therefore, HIF-1α inhibitors may be potential agents to treat canine
lymphoma. 相似文献
8.
Takeshi OHTA Shuichi KIMURA Masaya HIRATA Takahisa YAMADA Toshie SUGIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1327-1330
The Spontaneously Diabetic Torii (SDT) fatty rat, a model for obese type 2 diabetes,
shows bone quantitative abnormalities, namely low bone mineral density (BMD). The
objective of this study was to evaluate bone morphological changes, in particular
identifying the bone qualitative abnormalities, in the SDT fatty rat. Male SDT fatty rats
showed increases in total trabecular area and trabecular number and decreases in
trabecular thickness in cancellous bones of the proximal tibia, indicating trabecular
miniaturization. The SDT fatty rat is useful for investigation of pathophysiological
changes in bone quality in diabetic osteoporosis. 相似文献
9.
10.
Masatomi Hosoi Chuya Shinzato Masaya Takagi Shoko Hosoi-Tanabe Hideki Sawada Eri Terasawa Haruhiko Toyohara 《Fisheries Science》2007,73(2):385-394
ABSTRACT: Taurine is the primary osmolyte in marine molluscs, whose cellular osmo-conforming process is vital for environmental adaptation because of a lack of osmotic homeostasis. Here, cDNA cloning and expression, and functional analyses of taurine transporter (TAUT) from the giant Pacific oyster are reported on. The deduced amino-acid sequence of oyster TAUT (oyTAUT) showed 47–51% identity to those of vertebrate TAUT, whereas identity among the vertebrates is 78–95%. Functional analysis of oyTAUT expressed in Xenopus oocytes revealed that oyTAUT has a lower affinity and specificity for taurine and a requirement for higher NaCl concentration, compared with vertebrate TAUT. Taken together with similar functional properties of TAUT from mussel, indicated by our previous study, it is possible that these functional features reflect the internal environment of the molluscs (i.e. higher taurine and NaCl concentrations). Oyster taurine transporter mRNA expression was induced by not only hyper-osmotic stress, similar to other TAUT, but also hypo-osmotic stress. It is speculated that the expression in response to hypo-osmotic stress was induced by a substantial decrease in tissue taurine content following the decrease in the internal osmolality. 相似文献