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David E. Kime Mohammad A. S. Abdullah Miroslawa Sokolowska-Mikolajczyk Piotr Epler 《Fish physiology and biochemistry》1994,13(4):317-324
Carp ovarian tissue was incubated with 3H-17-hydroxyprogesterone in the presence of 0, 0.1, 1, 10, and 100 μg ml−1 unlabeled 17-hydroxyprogesterone. The pattern of metabolites formed showed a marked variation with substrate concentration.
Formation of glucuronide and sulphate conjugates was important only at low substrate concentration. At high substrate concentration
(10 and 100 μg ml−1) 17,20α-dihydroxy-4-pregnen-3-one was the major metabolite, but at intermediate concentrations polar 7α-hydroxypregnanetetrols
predominated. The results support the hypothesis that at low substrate concentrations conjugating, 5α-reducing and 7α-hydroxylating
enzymes, of high activity but low capacity, act as scavengers to deactivate any steroids formed during the relatively low
pituitary gonadotrophin secretions which are necessary for oocyte development, but that during the prespawning gonadotrophin
surge when high levels of substrate are present these enzymes are saturated and 17,20α-dihydroxy-4-pregnen-3-one (17,20αP)
becomes the major ovarian steroid. The possible role of 17,20αP during oocyte final maturation requires further examination. 相似文献
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The seedlings of the soybean ( Glycine max. (L.) Merr.) cv. Polan were investigated by subjecting them to water culture for a period of 14 d. To the Knop nutrient solution, lead was added as PbCl2 at four concentrations: 0, 10, 20 and 40 mg dm−3 . Observations of soybean leaf tissues were carried out by light microscopy, transmission electron microscopy and scanning electron microscopy. The Pb levels used in the present study reduced the area of cotyledons and leaf blades of the soybean plants. Pb-induced changes in the leaf epidermis structure involved a reduction in the cell size, more abundant wax coating, and an increase in the number of stomata and trichomes per unit area with simultaneous reduction in the size of the guard cells. The lead treatment resulted in the reduction in the thickness of the leaf blades, reduction in the area of xylem and phloem in the vascular bundles and in the diameter of the xylem vessels. Under Pb stress, the leaf mesophyll cells were characterized by the presence of altered chloroplasts with a reduced lamellar system and multidirectional pattern of the thylakoid system. Burst stroma of the thylakoid system and cracked chloroplast envelopes were also observed. The importance of the increase in the number of stomata and trichomes for plants under the metal stress was examined. 相似文献
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J. Chyb M. Sokolowska-Mikolajczyk T. Mikolajczyk M. Socha P. Epler 《Fish physiology and biochemistry》2003,28(1-4):91-92
The pituitaries of male and female common carps were subjected to the treatment with recombinant human activin A. Basal LH secretion decreased in females during wintering, but stimulated basal and sGnRH-induced LH secretion during ovulatory period. It stimulated LH secretion in males during wintering, but not during spawning, suggesting sex-specific effect of activin on LH secretion in common carp. 相似文献
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Humiczewska M 《Folia parasitologica》2002,49(3):221-226
Using histochemical and cytophotometric methods, enzymes responsible for the membrane transport (alkaline phosphatase, adenosine triphosphatase, and 5-nucleotidase) in the developing sporocyst of Fasciola hepatica (L., 1758) were studied. The most active metabolism occurred in the germ balls of sporocysts on the 8th and 15th days of development, which is associated with intensive proliferation and subsequently differentiation of embryos within the germ balls. 相似文献
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