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1.
Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3 总被引：17，自引：0，他引：17
C Holm T G Kirchgessner K L Svenson G Fredrikson S Nilsson C G Miller J E Shively C Heinzmann R S Sparkes T Mohandas 《Science (New York, N.Y.)》1988,241(4872):1503-1506
Hormone-sensitive lipase, a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis, is acutely controlled through reversible phosphorylation by catecholamines and insulin. The 757-amino acid sequence predicted from a cloned rat adipocyte complementary DNA showed no homology with any other known lipase or protein. The activity-controlling phosphorylation site was localized to Ser563 in a markedly hydrophilic domain, and a lipid-binding consensus site was tentatively identified. One or several messenger RNA species (3.3, 3.5, or 3.9 kilobases) were expressed in adipose and steroidogenic tissues and heart and skeletal muscle. The human hormone-sensitive lipase gene mapped to chromosome 19 cent-q13.3. 相似文献
2.
A new probe for the diagnosis of myotonic muscular dystrophy 总被引：11，自引：0，他引：11
R J Bartlett M A Pericak-Vance L Yamaoka J Gilbert M Herbstreith W Y Hung J E Lee T Mohandas G Bruns C Laberge 《Science (New York, N.Y.)》1987,235(4796):1648-1650
Myotonic muscular dystrophy (DM) is the most common muscular dystrophy, affecting adults as well as children. It is inherited as an autosomal dominant trait and is characterized by variable expressivity and late age-of-onset. Linkage studies have established the locus on chromosome 19. In order to identify tightly linked probes for diagnosis as well as to define in detail the DM gene region, chromosome 19 libraries were constructed and screened for restriction fragment length polymorphisms tightly linked to DM. A genomic clone, LDR152 (D19S19), was isolated that is tightly linked to DM; recombination fraction = 0.0 (95% confidence limits 0.0-0.03); lod score, 15.4. 相似文献
3.
Srinivas Somnath Pai Abdulaziz Anas Natamai Subramaniam Jayaprakash Prabhakaran Priyaja Balachandran Sreelakshmi Radhakrishnan Preetha Rosamma Philip Ambat Mohandas Isaac Sarogeni Bright Singh 《Aquaculture Research》2010,41(6):847-860
This study shows that the disease resistance and survival rate of Penaeus monodon in a larval rearing systems can be enhanced by supplementing with antagonistic or non‐antagonistic probiotics. The antagonistic mode of action of Pseudomonas MCCB 102 and MCCB 103 against vibrios was demonstrated in larval mesocosm with cultures having sufficient concentration of antagonistic compounds in their culture supernatant. Investigations on the antagonistic properties of Bacillus MCCB 101, Pseudomonas MCCB 102 and MCCB 103 and Arthrobacter MCCB 104 against Vibrio harveyi MCCB 111 under in vitro conditions revealed that Pseudomonas MCCB 102 and MCCB 103 were inhibitory to the pathogen. These inhibitory properties were further confirmed in the larval rearing systems of P. monodon. All these four probionts significantly improved larval survival in long‐term treatments as well as when challenged with a pathogenic strain of V. harveyi MCCB 111. We could demonstrate that Pseudomonas MCCB 102 and MCCB 103 accorded disease resistance and a higher survival rate in P. monodon larval rearing systems through active antagonism of vibrios, whereas Bacillus MCCB 101 and Arthrobacter MCCB 104 functioned as probiotics through immunostimulatory and digestive enzyme‐supporting modes of action. 相似文献
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5.
Balakrishnan Soumya Ameer Ahna Pazhur Mohandas Sowmya Maliyekkal Sajeevan Ambadi Kannan Bhaskaran Sathyabhama Anoop Singh Bright 《Aquaculture International》2022,30(4):2129-2144
Diseases are one of the most critical limiting factors in aquaculture. Recirculating aquaculture systems (RAS) are one of the most functionally viable sustainable aquaculture production systems adopted world over. In the event of any eventuality caused by bacterial pathogens, antibiotics or other antibacterial agents cannot be applied due to the vulnerability of biological filters which form an integral part of the RAS. Because of this, newer drugs are required for the effective control of diseases in RAS which would not interfere with the activity of nitrifying bacteria used in the biological filters. The antagonistic activity of pyocyanin, a bioactive compound produced by Pseudomonas aeruginosa, against vibrios is well established. The purpose of this study was to prove the effectiveness of pyocyanin as an aquaculture drug for application in RAS by employing a pilot-scale shrimp culture under RAS. It was noted that at the concentration of 5 mg L?1, pyocyanin could significantly bring down the population of Vibrio spp. in RAS without affecting noticeably the other natural heterotrophic bacteria. Also, pyocyanin at this concentration did not inhibit the activity of nitrifying bacterial consortia used in the SBSBR (stringed bed suspended bioreactor) of RAS. The reared shrimp (Penaeus monodon) showed 100% survival after the application of pyocyanin, besides exhibiting normal health signs. Pyocyanin was non-toxic to the shrimp hybrid cell line (PmLyO-Sf9) at the concentration required for its application in disease management (IC50?=?419.26 mg L?1). The present study has demonstrated that pyocyanin is effective as an environment-friendly and safe aquaculture drug for the application in RAS-based shrimp culture to control Vibrio spp. without impeding nitrification established through the deployment of nitrifying bioreactors.
相似文献6.
Jose S Jayesh P Sudheer NS Poulose G Mohandas A Philip R Singh IS 《Journal of fish diseases》2012,35(5):321-334
Shrimp cell lines are yet to be reported and this restricts the prospects of investigating the associated viral pathogens, especially white spot syndrome virus (WSSV). In this context, development of primary cell cultures from lymphoid organs was standardized. Poly-l-lysine-coated culture vessels enhanced growth of lymphoid cells, while the application of vertebrate growth factors did not, except insulin-like growth factor-1 (IGF-1). Susceptibility of the lymphoid cells to WSSV was confirmed by immunofluoresence assay using monoclonal antibody against the 28 kDa envelope protein of WSSV. Expression of viral and immune-related genes in WSSV-infected lymphoid cultures could be demonstrated by RT-PCR. This emphasizes the utility of lymphoid primary cell culture as a platform for research in virus-cell interaction, virus morphogenesis, up and downregulation of shrimp immune-related genes, and also for the discovery of novel drugs to combat WSSV in shrimp culture. 相似文献
7.
Victor Stalinraj Koyadan Kizhakedath Vijayan Mohandas Sanjuktha Changaramkumarth Paran Balasubramanian Shankar Vinayakarao Alavandi Thairiyam Chinnappan Santiago 《Aquaculture Research》2009,40(2):129-138
The objective of this study was to investigate the reason for variation in the virulence of white spot syndrome virus (WSSV) from different shrimp farms in the Southeast coast of India. Six isolates of WSSV from farms experiencing outbreaks (virulent WSSV; vWSSV) and three isolates of WSSV from farms that had infected shrimps but no outbreaks (non‐virulent WSSV; nvWSSV) were collected from different farms in the Southeast coast of India. The sampled animals were all positive for WSSV by first‐step PCR. The viral isolates were compared using histopathology, electron microscopy, SDS‐PAGE analysis of viral structural proteins, an in vivo infectivity experiment and sequence comparison of major structural protein VP28; there were no differences between isolates in these analyses. A significant observation was that the haemolymph protein profile of nvWSSV‐infected shrimps showed three extra polypeptide bands at 41, 33 and 24 kDa that were not found in the haemolymph protein profile of vWSSV‐infected shrimps. The data obtained in this study suggest that the observed difference in the virulence of WSSV may not be due to any change in the virus, rather it could be due to the shrimp defence system producing certain factors that help it to accommodate the virus without causing any mortality. 相似文献
8.
Sukhada Mohandas 《The Journal of Horticultural Science and Biotechnology》2013,88(5):514-518
SummaryPapaya (Carica papaya L.) cv. Coorg Honey Dew is one of the most popular cultivars grown in Southern India, but it requires high doses of inorganic phosphorus (P) fertilisation for growth. Arbuscular mycorrhizal fungi (AMF) are known to improve plant growth and nutrient uptake, especially the uptake of P and micronutrients. As papaya plants respond well to high levels of P, inoculation with AMF was studied to see if AMF could fulfill the requirement for P in plants grown under field conditions. Papaya seedlings (n = 36 per AMF) were colonised separately, in polybags, for 45 d by two species of AMF, Glomus mosseae and G. fasciculatum. Seedlings were then transplanted to the field, with uninoculated seedlings as controls, in a replicated randomised block design. Three levels of P were applied [50, 75, or 100% of the recommended dose (240 g plant–1 year–1) of P fertiliser, as super-phosphate]. Plants were studied for root colonisation by AMF, for growth parameters, and for leaf nutrient contents (especially, P, Zn, and Cu). Acidic and alkaline phosphatase activities in the roots of AMF-colonised plants were recorded as these enzymes are involved in the mobilisation of P. The yields of plants up to 18 months-old, and fruit quality, measured by total soluble solids contents (TSSC) and β-carotene contents, were recorded. AMF-inoculated plants performed better than uninoculated control plants at all levels of P applied. G. mosseae was more effective at improving plant growth, fruit yield, and P and Zn contents than G. fasciculatum at the 75% and 50% P-levels. Cu contents increased at all P-levels in G. fasciculatum-colonised plants. Total soluble solids contents showed marginal improvements at the 75% P level with both fungi. β-carotene contents increased significantly in G. mosseae-colonised plants at the 50% and 75% P-levels, and in G. fasciculatum-colonised plants at the 75% P-level. The feasibility of applying on-farm produced AMF inoculum to improve papaya cultivation and to save 25% of the P applied during papaya cultivation is discussed. 相似文献
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The apicomplexan parasites Babesia and Plasmodium are related, yet phylogenetically distinct haemoprotozoa that infect red blood cells and cause severe diseases of major human and veterinary importance. A variety of cellular and molecular interactions are pivotal in many aspects of the pathogenicity of these two parasites. Comparison of the cellular and molecular mechanisms that culminate in accumulation of parasitised red blood cells in the microvasculature of cattle infected with Babesia bovis (babesiosis) and humans infected with Plasmodium falciparum (falciparum malaria) is particularly instructive given the striking similarities in the pathophysiology of these two important medical and veterinary diseases. While such adhesive phenomena have been studied extensively in malaria, they have received relatively little attention in babesiosis. In this review, we summarise the findings of more than 25 years of research into cellular adhesive phenomena in malaria and speculate on how this body of work can now be applied to Babesia parasites. Such information is fundamental if we are to learn more about the biology of Babesia parasites, the cellular and molecular mechanisms by which they cause infection and disease and how to develop novel therapeutic strategies or vaccines for both Babesia and malaria infections. 相似文献
10.
Roberta Di Terlizzi Patrick G. Gallagher Narla Mohandas Laurie A. Steiner Karen S. Dolce Xinhua Guo Melinda J. Wilkerson Steven L. Stockham 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(1):52-58
Abstract: A 5‐year‐old, spayed female, mixed‐breed dog with persistent elliptocytosis was evaluated at the Veterinary Medical Teaching Hospital at Kansas State University. The elliptocytosis was asymptomatic and was detected during the evaluation of lameness. When subjected to shear stress in an ektacytometer, the dog's erythrocytes had reduced cellular deformability and erythrocyte membranes had decreased mechanical stability. Analysis of erythrocyte membrane spectrin by nondenaturing gel electrophoresis revealed an increased amount of spectrin dimers, indicating a defect in spectrin self‐association. DNA analysis detected a β‐spectrin mutation in codon 2110 in which threonine was replaced by methionine. This mutation likely altered the molecular structure of the erythrocyte membrane, leading to impaired spectrin self‐association and elliptocyte formation. 相似文献