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Minimal Set of Metabolic Pathways Suggested from the Genome of Onion Yellows Phytoplasma 总被引：1，自引：0，他引：1
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Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(2):173-176
The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 相似文献
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Abstract. Changes in the properties of urine and blood of carp with sekoke disease were studied. With the progress of this disease, the parameters of blood and particularly urine changed significantly. After 120 days, urine flow, osmolarity and concentrations of inorganic ions (K+ , Ca2+ , Mg2+ , Cl− ) and organic compounds (ammonium, creatine and creatinine, protein, glucose) in the experimental group showed higher values than those of the controls. Even after 60 days, when clinical signs of sekoke disease were not evident, abnormally high concentrations of ammonium, protein and glucose in the urine were found in the experimental group. 相似文献
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Abstract. Changes in blood properties and the chemical components, histology and isometric twitch tension of lateral muscle were studied in carp, Cyprinus carpio L., showing clinical signs of sekoke disease induced by a diet containing oxidized oil. When compared to normal fish, sekoke fish were found to have significantly lower mean blood pH, haematocrit, haemoglobin concentration and muscle water content. However, plasma lactate, lipoperoxide and total lipid concentrations were significantly higher in sekoke fish. It was found that in sekoke fish the maximum tension and the number of isometric twitches leading to complete fatigue of lateral muscle were greatly reduced. Histological studies confirmed that these reductions were related to the degree of structural damage within this tissue. 相似文献
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The Complete Nucleotide Sequence of Potato virus X Strain OS: The First Complete Sequence of a Japanese Isolate 总被引：3，自引：0，他引：3
Satoshi KAGIWADA Yasuyuki YAMAJI Hitomi NAKABAYASHI Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(1):94-98
The complete nucleotide sequence of the genomic RNA of a Japanese isolate of Potato virus X (PVX), strain OS (PVX-OS), was determined. The PVX-OS genome is 6435 nucleotides in length, excluding the 3′ poly(A) sequence,
and encodes for five open reading frames (ORFs), similar to other reported PVX strains. Comparisons of the nucleotide sequence
of PVX-OS with those of European and South American PVX strains revealed that the PVX-OS genomic sequence showed 95-96% homology
with European strains and 77-78% with South American strains. Phylogenetic analysis of PVX-OS and other PVX strains found
that PVX-OS clusters with European strains. This is the first report on the complete nucleotide sequence of a PVX strain from
Japan.
Received 10 May 2001/ Accepted in revised form 26 October 2001 相似文献
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Shin-ichi MIYATA Ken-ichiro FURUKI Toshimi SAWAYANAGI Kenro OSHIMA Tsutomu KUBOYAMA Tsuneo TSUCHIZAKI Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(1):62-67
The complete region of a putative streptomycin operon (str operon) of onion yellows (OY) phytoplasma, a phytopathogenic mollicute, was isolated and sequenced. This operon contains four
genes, rps12, rps7, fus, and tuf, encoding ribosomal proteins S12 and s7, elongation factor (EF) -G, and EF-Tu, respectively. These four genes constitute the
str operon in non-mollicute bacteria, such as Escherichia coli and Bacillus subtilis. In two species of mollicute Mycoplasma, the tuf gene was reported not to be included in this operon, but was located apart, indicating that the gene arrangement of this operon
in phytoplasmas resembles that of B. subtilis more than that of Mycoplasma spp. In addition, the deduced amino acid sequence of EF-G of phytoplasmas also resembles that of B. subtilis more than that of Mycoplasma spp. These results suggest that analyses of the gene organization and sequence of the phytoplasma genome will provide valuable
insights into evolutionary relationships among the culturable mollicutes, phytoplasmas and other Gram-positive bacteria.
Received 25 April 2001/ Accepted in revised form 21 August 2001 相似文献
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