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1.
Jazayeri M Soleimanjahi H Fotouhi F Pakravan N 《Comparative immunology, microbiology and infectious diseases》2009,32(5):453-461
Herpes simplex virus type 2 is the most common infectious agent in humans that causes genital herpes disease and vaccination is a desirable method to prevent herpes infections. An effective therapeutic vaccine will need to elicit virus-specific immune responses. The route of immunization has important role in immune responses. In this study, DNA vaccine encoding glycoprotein D of herpes simplex virus type 2 (HSV-gD2) was prepared and injected via intramuscular and footpad routes to determine the optimal method of delivery for immune stimulation. The control manipulation of immune response by concerning route of administration is highly appreciated issue by researches. Although DNA vaccine containing HSV-gD2 is effective in both intramuscular and footpad injection routes, the latter could induce significantly higher cellular responses against HSV-2. 相似文献
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Sina Ghanbari Ahmad Nooshkam Barat Ali Fakheri Nafiseh Mahdinezhad 《Journal of Crop Science and Biotechnology》2018,21(5):435-441
18 soybean genotypes were examined to investigate the relationships between some principal attributions of morphology with seed yield per soybean, by Random Complete Block Design (RCBD) study. This study was also carried out three replicates to gain reliable results. The results of variance analysis indicated that, there were significance differences among all soybean genotypes. Moreover, the results of correlated analysis revealed that biological yield (0.96), harvest index (0.92), and number of branches (0.92) had the uttermost correlation with seed yield. To data factor analysis, four independent variables justified 99 percent of all data. The first variable, seed yield, justified 96 percent of entire variance. Multiple-Regression Model with method Analytical Regression Model (step-by-step) was utilized to examine soybean seed yield. This model proved that biological yield, thousand seed weight, and harvest index entered into model respectively and justified 98.85 percent of variation of seed yield. Correlated coefficients of considered attributions were equal to 0.96, 0.78, and 0.92, respectively. All of these indexes had significant at 1% in statistical process. Therefore, these traits can be notability used in soybean breeding programs. Also, accordance to cluster analysis, the sample was divided into three groups. 相似文献
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In the present study, three DNA extraction procedures were examined to determine which might yield DNA from Grape leaves suitable for molecular analysis for RAPD, SSR. AFLP and etc analysis. The three methods examined were: the miniprep procedure and the modified CTAB for difficult species and protocol CTAB. Only the modified CTAB method consistently yielded DNA suitable for Polymerase Chain Reaction (PCR) amplification, regardless of plant growing conditions or leaf age. The quality and quantity of extracted genomic DNA gained from these methods are deliberated by means UV biophotometer, electrophoresis in 1.2% agarose gel and PCR. In this regard, application chosen for young and mature leaves, the most value of qualified DNA, is extracted from fully expanded leave when PVP was added to the extraction buffer. This same procedure also yielded PCR-amplifiable DNA from various other perennial, woody species and from other fruit species such as apple (Malus domestica), cherry (Prunus avium), peach (Prunuspersica), plum (Prunus domestica). DNA yield from this procedure is high (up to 1 mg g(-1) of leaf tissue). DNA is completely digestible with restriction endonucleases and amplifiable in the Polymerase Chain Reaction (PCR). 相似文献
5.
Mahdi Jaberi Behzad Gharehyazi Mahmood Khosrowchahli Ali Sharafi Nafiseh Aboofazeli 《The Journal of Horticultural Science and Biotechnology》2018,93(2):204-208
Plant regeneration ability was studied in the medicinal-ornamental plant, Cosmos bipinnatus ‘Sonata white’, which is a dicotyledonous recalcitrant plant to shoot induction. Cotyledons were used as sources of explants to investigate plant regeneration. High frequency of direct shoot induction was obtained when BA (5 mg/l) and AgNO3 (5 mg/l) were used in combination with 20 mg/l adenine sulphate (73.8%) in Murashige and Skoog (MS) medium. The highest shoot number per explant (5.7) was induced on MS medium supplemented with 5 mg/l BA, 5 mg/l AgNO3, and 40 mg/l adenine. Eight week-old shoots were transferred to root induction media containing MS and half-strength MS medium with different concentration of IBA. The highest rate of root induction (70.8%) was obtained on half-strength MS medium with 1.5 mg/l IBA within four weeks. The plantlets were transferred to pot and kept in the greenhouse condition. Seventy percent of the plantlets successfully acclimatised.
Abbreviations: BA, 6-benzylaminopurine; IBA, Indole-3-butyric acid; MS, Murashige and Skoog; PGRs, plant growth regulators. 相似文献
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This study was conducted to determine how land management in three ecosystems including forest, rangeland, and saline desert soils affects the activity and diversity of isoenzymes, and the kinetic and thermodynamic parameters of soil cellulase. Cellulase was chosen because it hydrolyzes cellulose, a major plant substance entering the soils. Soil samples were collected in October 2009 from a native forest, a native rangeland, and a native saline desert as well as adjacent cultivated fields in central Iran. Land use changes of the native forest, rangeland, and saline desert soils resulted in 79%, 47%, and 73% reductions in cellulase activity, respectively. The Michaelis constant (Km) increased but the maximum catalytic velocity (Vmax) decreased for soil cellulase as a result of cultivation in the study areas. The thermodynamic parameters (Ea and Q10) of soil cellulase were also affected by cultivation. Higher Ea and Q10 values were obtained for the native soils, but the lower values belonged to the cultivated soils. The cellulase zymographic pattern revealed different cellulase isoenzymes in the soils. The prevailing cellulase isoenzymes in the soils were found to have molecular weights of 62 and 74 kDa in the native forest, 86 kDa in the cultivated forest, 55 and 65 kDa in the native rangeland, and 72 kDa in the reclaimed saline areas. Evidence reveals that the sensitivity of soil cellulase kinetic and thermodynamic parameters to land management seems to be due to differences in the cellulase isoenzymes present in soils. 相似文献
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Najmeh Zarei Hosnieh Ghasemi Mahsa Nayebhashemi Mozhgan Zahmatkesh Monire Jamalkhah Nafiseh Moeinian Zahra Mohammadi Somayeh Enayati Vahid Khalaj 《Iranian Biomedical Journal》2021,25(4):255
Background:The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant proteins, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which its deletion extends RLS. Herein, a los1-deficient strain of P. pastoris was generated and characterized. Methods:A gene disruption cassette was prepared and transformed into an anti-CD22-expressing strain of P. pastoris. A δ los1 mutant was isolated and confirmed. The drug sensitivity of the mutant was also assessed. The growth pattern and the level of anti-CD22 ScFv expression were compared between the parent and mutant strains.Results:The los1 homologue was found to be a non-essential gene in P. pastoris. Furthermore, the susceptibility of los1 deletion strain to protein synthesis inhibitors was altered. This strain showed an approximately 1.85-fold increase in the extracellular level of anti-CD22 scFv (p < 0.05). The maximum concentrations of total proteins secreted by δ los1 and parent strains were 125 mg/L and 68 mg/L, respectively.Conclusion:The presented data suggest that the targeted disruption of los1 homologue in P. pastoris can result in a higher expression level of our target protein. Findings of this study may improve the current strategies used in optimizing the productivity of recombinant P. pastoris strains. Key Words: Aging, Longevity, Pichia pastoris, Recombinant proteins 相似文献
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In this study, the role of the globin moiety in the structure of this pigment has been evaluated, using myoglobin and hemin as model systems. After the synthesis of the cured pigment from the compounds used in this study, the absorption spectra, Fourier transform infrared spectroscopy (FTIR), and electrospray ionization (ESI)/MS spectroscopy were used to evaluate the chemical structure. Results indicated that the UV/visible, IR absorption, and mass spectroscopy of the cured pigment produced from myoglobin and its counterpart without the globin moiety, hemin, are different. Whereas myoglobin produced mononitrosylheme, hemin converted to dinitrosylheme, but probably the second nitric oxide group attached to the propionate side chain of the heme ring. It seems that the globin moiety protected heme ring against the second nitric oxide group. 相似文献
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Adel Spotin Soheila Rouhani Parnazsadat Ghaemmaghami Ali Haghighi Mohammad Reza Zolfaghari Aref Amirkhani Mahin Farahmand Ali Bordbar Parviz Parvizi 《Iranian Biomedical Journal》2015,19(3):149-159
Background:
Molecular diversity of Leishmania major and its morphological changes have become a controversial issue among researchers. Some aspects of polymorphic shapes of amastigotes in clinical manifestations along with molecular variation were evaluated among suspected patients of some exceptional zoonotic cutaneous leishmaniasis locations in Northern Khuzestan, Southwestern Iran.Methods:
Suspected patients (n = 165) were sampled in zoonotic cutaneous leishmaniasis foci over two consecutive years during 2012-2014. Prepared smears were stained, scaled and measured by ocular micrometer. DNA was extracted from smears; ITS-rDNA and Cytochrome b (Cyt b) markers were amplified, and PCR products were digested by BsuR1 restriction enzyme. Then the RFLP and sequencing were employed.Results:
Only L. major was identified in patients containing regular amastigotes'' shapes (oval or round) with a size of 2-4 µm in each of classical wet, dry, mixed lesions. Meanwhile, irregular shapes (spindle, pear, or cigarette) were observed separately in non-classical wet lesions with more than 4 µm. Interestingly, a few amastigotes with an external flagellum were observed in some lesions. All sequenced ITS-rDNA and Cyt b genes of L. major did not show any molecular variation (χ 2 P > 0.05), including only one common haplotype (GenBank access no. EF413075).Conclusion:
Findings proved that unlike other endemic foci, there is not a meaningful correlation between phenotypic and genotypic features of L. major isolates. This study is considered as the first comprehensive report to incriminate morphometric shapes of L. major amastigotes, which enhances our knowledge concerning their relevance with various clinical appearances and genotypic traits. Key Words: Leishmania major, Nuclear gene, Mitochondrial gene, Amastigote shapes, Iran 相似文献10.
Malayeri BE Chehregani A Yousefi N Lorestani B 《Pakistan journal of biological sciences: PJBS》2008,11(3):490-492
The aim of present study was to identify accumulator plants that are effective for phytoremediation. We chose a mine of Iron and Copper named Hame Kasi that located western north of Hamedan city as a polluted area. In this region concentration of heavy metals is several times more than non-polluted area. Seventeen plant species and 6 soil samples were collected from this region for determination of heavy metals content. Atomic Absorption Spectrophotometer (AAS) was used for analysis of heavy metals in soil and plant samples, then tested plant species were grouped on the basis of their accumulation capability of heavy metals. The results of this research showed that there are some hyper accumulator plants in this area that can concentrate heavy metals in their different parts thus they can be used for remediation of polluted area. 相似文献