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1.
Sivakumar P Sankar M Nambi PA Praveena PE Singh N 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(10):506-509
The mesenteric lymph nodes (MLN) of buffaloes (n = 100) were examined for the presence of parasitic infection. The nymphal stage of Linguatula serrata was observed in two buffaloes. A single white-coloured nymph with transversely striated spines on a segmented body, two pairs of oral suckers and hooks was observed in the MLN. The morphometrics of the nymphs were studied. The affected lymph nodes were grossly enlarged with cyst and showed pathological lesions of fibroblastic reaction with a mild underlying inflammatory zone. 相似文献
2.
We compared arbuscular mycorrhizal (AM) and dark septate endophyte (DSE) fungal associations in 2 crops and 31 weeds commonly occurring in pulp and paper mill effluent irrigated and well-water irrigated soils. Soil pH, organic C, N, P and K, were higher in pulp and paper mill effluent irrigated than in well-water irrigated soils. In contrast, the average AM fungal colonization, root length with AM fungal hyphae/hyphal coils, spore numbers and diversity were lower in pulp and paper mill effluent irrigated soils compared to well-water irrigated soils. However, no significant variation was found in DSE fungal colonization nor root length with AM fungal arbuscules/arbusculate coils and vesicles between pulp and paper mill effluent irrigated and well-water irrigated soils. A significant negative correlation existed between AM and DSE fungal colonization in both effluent and well-water irrigated soils. Twelve AM fungal spore morphotypes belonging to Acaulospora, Dentiscutata, Glomus, Racocetra and Scutellospora were isolated from the well-water irrigated soils, whereas spores of six morphotypes were isolated from effluent irrigated soils. AM fungal spore numbers were correlated significantly and positively to AM fungal colonization in effluent and well-water irrigated soils. 相似文献
3.
P. N. Sivalingam D. K. Samadia Dhurendra Singh H. K. Changal Hanif Khan S. K. Sharma 《Genetic Resources and Crop Evolution》2011,58(7):1095-1103
Sixteen germplasm accessions of Prosopis cineraria with suitable horticultural traits were identified from north-western Rajasthan, India, propagated clonally by budding on
seedling rootstock and maintained in the field gene bank. Morphological characterization of seven-year-old trees of these
accessions by 21 traits indicated a lot of variation among the accessions tested. Higher number of flowers per raceme was
found in accession CIAH/K2, higher width of ripened pod in CIAH/K5, higher number of seeds per pod in CIAH/K12 and a higher
weight of seed per pod in CIAH/K6. Overall, CIAH/K16 was found to be a superior genotype for most of the useful traits. High
significant positive correlation was obtained with traits useful for horticultural values. Out of 62 random decamer primers
for random amplification (RAPD) reaction, and four minisatellite core sequence for direct amplification of minisatellite DNA
(DAMD) screened with these accessions, 12 RAPD and 2 DAMD primers were found polymorphic. Average polymorphism resolved by
these markers among the accessions was 93.2%. Genetic diversity revealed by Jaccard’s co-efficient was between 0.11 and 0.77,
and four major clusters were identified among these accessions by phylogenetic analysis using NTSYSpc-2.02e software. This
study shows the existence of high genetic diversity within these accessions. 相似文献
4.
A survey was made on the content of nine trace metals in waters of 12 predetermined coastal stations in the Island of Penang, Malaysia, with the object of establishing mussel culture farms in the near future. The results indicated low concentrations of these trace elements except for Cr and Ni, which ranged from trace level to 35 ppm and BDL (below detectable level) to 2.07 ppm, respectively. These values are slightly above the upper limits (Cr, 0.05 and Ni, 1.0 ppm) set by the national Division of Environment, Ministry of Science, Technology and Environment, under the Environmental Quality (Sewage and Industrial Effluents) Regulations 1978.Investigations on tissue level bioaccumulated trace metal contents in mussel from an experimental culture farm indicated BDL values for As in all tissues, Cd highest in the intestines and stomach (6.24 ppm), Co in the mantle (59.85 ppm), Cr in the siphon (1869.85 ppm), Cu in the mantle (23.94 ppm), Fe in the intestines and stomach (218.82 ppm), Mn in the style (365.28 ppm), Ni in the mantle (23.94 ppm), Pb in the mantle (279.3 ppm) and Zn in the style (152.2 ppm). However, culture experiments at 10 ppm concentration stresses of the different trace elements over a 48-h period did not result in a similar tendency in tissue bioconcentration, except for Cd (378.35 ppm) and Fe (419.66 ppm) in the intestine and stomach, and Mn (748.5 ppm) in the style. Co was highest in the style (187.35 ppm), Cr in the gill (2924.91 ppm), Cu in the gill (457.22 ppm), Ni in the intestines and stomach (222.44 ppm), Pb in the gill (1897.2 ppm), and Zn in the mantle (1077.73 ppm).Short-term stress studies over a 48-h period with trace metal concentrations ranging between 10 and 300 ppm indicated the patterns of biodeposition for Cd, Cr, Co, Pb, Mn and Zn to be closely related even though their toxicity levels varied. Cu indicated a bioaccumulation maximum after 12 h of incubation followed by a regulatory mechanism, while Fe and Ni demonstrated a drastic spiked absorption after 6 h exposure followed by the normal trend of biodeposition as observable for the other trace metals. The biodeposition factors at most instances, were highest for the 10 ppm concentration cultures of 48 h. 相似文献
5.
M A Farook N Madan G Taju S Abdul Majeed K S N Nambi N Sundar Raj S Vimal A S Sahul Hameed 《Journal of fish diseases》2014,37(8):703-710
White tail disease (WTD) caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) is a serious problem in prawn hatcheries. The gene for capsid protein of MrNV (MCP43) was cloned into pRSET B expression vector. The MCP43 protein was expressed as a protein with a 6‐histidine tag in Escherichia coli GJ1158 with NaCl induction. This recombinant protein, which was used to raise the antiserum in rabbits, recognized capsid protein in different WTD‐infected post‐larvae and adult prawn. Various immunological methods such as Western blot, dot blot and ELISA techniques were employed to detect MrNV in infected samples using the antiserum raised against recombinant MCP43 of MrNV. The dot blot assay using anti‐rMCP43 was found to be capable of detecting MrNV in WTD‐infected post‐larvae as early as at 24 h post‐infection. The antiserum raised against r‐MCP43 could detect the MrNV in the infected samples at the level of 100 pg of total protein. The capsid protein of MrNV estimated by ELISA using anti‐rMCP43 and pure r‐MCP43 as a standard was found to increase gradually during the course of infection from 24 h p.i. to moribund stage. The results of immunological diagnostic methods employed in this study were compared with that of RT‐PCR to test the efficiency of antiserum raised against r‐MCP43 for the detection of MrNV. The Western blot, dot blot and ELISA detected all MrNV‐positive coded samples as detected by RT‐PCR. 相似文献
6.
P. N. Sivalingam Dhurendra Singh Sarita Chauhan 《Genetic Resources and Crop Evolution》2012,59(2):305-316
Twenty two germplasm accessions of Cordia myxa were collected from Rajasthan and established at the field gene bank for conservation and evaluation. Morphological characterization
of 10 year-old trees for 17 traits indicated wide variations among the accessions tested. Higher number of flowers per cyme
was found in accession ACHM11 and higher pulp:stone ratio in AHCM25. Overall, AHCM22 was found to be a superior germplasm
line for most of the horticulturally useful traits among the accessions tested as it had higher percent of fruit set, pulp:stone
ratio and fruit weight. High significant positive correlation was obtained between leaf, fruit characters and pulp:stone ratio.
However, these characters were found to be negatively correlated with number of flowers per cyme. Out of 50 random decamer
primers used for random amplification (RAPD), 25 were polymorphic. Average polymorphism resolved by these markers among these
accessions was 69.8% with an average polymorphic information content of 0.43. Genetic diversity revealed by Jaccard’s co-efficient
was between 0.44 and 0.94, and three major clusters were identified among these accessions by phylogenetic analysis using
NTSYSpc-2.02e software. RAPD markers associated with leaf size and pulp:stone ratio were also identified. This study shows
the existence of high genetic diversity among these accessions. 相似文献
7.
Development,distribution and expression of a DNA vaccine against nodavirus in Asian Seabass,Lates calcarifier (Bloch, 1790)
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S Vimal M A Farook N Madan S Abdul Majeed K S N Nambi G Taju N Sundar raj S Venu R Subburaj A R Thirunavukkarasu A S Sahul Hameed 《Aquaculture Research》2016,47(4):1209-1220
Fish nodavirus (betanodavirus), a viral pathogen responsible for viral nervous necrosis (VNN) was isolated from infected Asian sea bass (Lates calcarifer). The distribution, clearance and expression of nodavirus vaccine, on the basis of DNA vaccine (pFNCPE42 DNA‐pcDNA3.1) construction, were analysed in tissues of the Asian seabass by PCR, RT‐PCR, ELISA and Immunohistochemistry. Fish immunized with a single intramuscular injection of 20 μg of the pFNCPE42‐DNA vaccine showed a significant increase in the serum antibody level in the 3rd week after vaccination, compared to control eukaryotic expression vector pcDNA3.1 vaccinated fish. Results from PCR studies indicated that the vaccine‐containing plasmids were distributed in heart, intestine, gill, muscle and liver 10 days after vaccination. Clearance of pFNCPE42‐DNA vaccine was studied at 10, 25, 50, 75 and 100 days of post vaccination (d p.v). At 100 days p.v. pFNCPE42‐DNA was cleared from muscle of vaccinated sea bass. In vitro and in vivo expression of fish nodavirus capsid protein gene (FNCP) was determined by fluorescent microscopy. Asian seabass was immunized with pFNCPE42‐DNA vaccine at a dose of 20 μg per fish and were challenged with betanodavirus by intramuscular injection. The vaccinated seabass was protected from nodaviral infection and 77.33% of relative percent survival (RPS) was recorded. 相似文献
8.
N Sundar Raj K S Nathiga Nambi S Abdul Majeed G Taju S Vimal M A Farook A S Sahul Hameed 《Journal of fish diseases》2012,35(12):917-925
An attempt was made to determine the replication efficiency of white spot syndrome virus (WSSV) of shrimp in different organs of freshwater rice‐field crab, Paratelphusa hydrodomous (Herbst), using bioassay, PCR, RT‐PCR, ELISA, Western blot and real‐time PCR analyses, and also to use this crab instead of penaeid shrimp for the large‐scale production of WSSV. This crab was found to be highly susceptible to WSSV by intramuscular injection. PCR and Western blot analyses confirmed the systemic WSSV infection in freshwater crab. The RT‐PCR analysis revealed the expression of VP28 gene in different organs of infected crab. The indirect ELISA was used to quantify the VP28 protein in different organs of crab. It was found that there was a high concentration of VP28 protein in gill tissue, muscle, haemolymph and heart tissue. The copy number of WSSV in different organs of infected crab was quantified by real‐time PCR, and the results revealed a steady increase in copy number in different organs of infected crab during the course of infection. The viral inoculum prepared from different organs of infected crab caused significant mortality in tiger prawn, Penaeus monodon (Fabricius). The results revealed that this crab can be used as an alternate host for WSSV replication and production. 相似文献
9.
Palaiyur N. Sivalingam Dilip K. Samadia Dhurendra Singh Sarita Chauhan 《The Journal of Horticultural Science and Biotechnology》2016,91(4):353-361
Prosopis cineraria (L.) Druce, commonly called khejri, is an important multipurpose, leguminous tree of the Thar Desert. ‘Thar Shoba’ is a variety of khejri without thorns which was developed as a vegetable. In order to develop markers for varietal identification, 80 RAPD primers were tested, of which nine were polymorphic between ‘Thar Shoba’ and trees in a natural population of khejri. Phenetic analysis revealed that ‘Thar Shoba’ formed a separate cluster from the natural population with similarity coefficients of 0.57–0.78. The 10-mer primers OPBE05, OPBA13, OPA12, and OPA14 were each found to differentiate ‘Thar Shoba’ from the natural population by one amplicon. These differential amplicons were sequenced and SCAR markers were developed based on the sequences that differentiated ‘Thar Shoba’ from the natural population. Natural populations of khejri from different regions of the Thar Desert, and clonally multiplied ‘Thar Shoba’ plants in the ICAR-CIAH bud-wood genebank, were used to validate these primers. These markers will be useful in further developing the bud-wood genebank, and for certification, identification, and protection of the khejri variety ‘Thar Shoba’. 相似文献
10.
Cracking of seed coats in soybean (Glycine max (L.) Merr.) deteriorates the external appearance of seeds and reduces their commercial value. Two types of cracking have
been reported that occur in some cultivars: Type I with irregular cracks and Type II with net-like cracks. This study was
conducted to determine the genetic basis of net-like cracking. Genetic analysis was performed using F1 plants produced by crossing Uzuramame, a Japanese landrace with black seed coats having net-like cracking and a Clark mutant
with black seed coats, their F2 population and F3 lines. Degree of cracking in individual plants was calculated by averaging cracking index (no cracking: 0 to severe cracking:
4) of total or 100-seed samples (average cracking index, ACI). Uzuramame exhibited intense cracking, whereas the Clark mutant
showed slight cracking. Intermediate degree of cracking in F1 plants suggested incomplete dominance. ACI of F2 plants was continuously distributed. Gene number involved was estimated to be 1.4 by Wright's method. All F3 lines derived from F2 plants with ACI more than 2.8 displayed severe cracking phenotypes. In contrast, F3 lines derived from F2 plants with ACI less than 2.8 segregated from low to high ACI (0.5 to 3.2). When F2 plants were classified as slight (ACI<2.8) or severe (ACI>2.8) cracking, the frequency distribution of the F2 plants fitted to a 3:1 ratio. Genotypes of SSR marker Satt264 that is closely linked to SoyPRP1 locus for proline-rich cell
wall protein had a minor effect on ACI. Further, seed weight was positively associated with ACI (r =0.46**). Our results suggest that net-like cracking is controlled primarily by a major gene, and SoyPRP1 and gene(s) contributing
to seed weight may have minor effects on the intensity of cracking.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献