An attempt to eradicate bovine leukemia virus infection in a public pasture     

H Itoh  N Ogasawara  K Ohshima  K Okada  S Numakunai  Y Seimiya 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(3):661-663

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Evaluation of Live Feeds for Larval and Postlarval Mahimahi Coryphaena hippurus   总被引：3，自引：0，他引：3  

Syd  Kraul  Alan  Nelson Karen  Brittain Harry  Ako Amy  Ogasawara 《Journal of the World Aquaculture Society》1992,23(4):299-306

Live hatchery feeds were assayed for fatty acids (FA), amino acids (AA), and their ability to support growth and survival of larval and postlarval mahimahi Coryphaena hippurus at two different hatchery stages. Euterpina acuritrons copepods (C), mahimahi yolk-sac larvae (YSL), Artemia parthenogenica brine shrimp nauplii (BSn), A. parthenogenica juveniles (BSj), and Brachionus plicatilis rotifers (R) were assayed, using several enrichment media. There was little difference in AAs among feeds.
Levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were about 10 times higher in YSL than in their feeds. This explains previous findings where first stage larval survival (0–9 days) was not affected by feed HUFA levels. Second stage survival was significantly higher when larvae were fed copepods. Enrichment with 100 ppm SuperSelco greatly improved the survival of larvae that were fed brine shrimp. The even higher omega-3 fatty acids found in copepods appear to be important for survival of larvae under more stressful conditions.
Brine shrimp juveniles enriched with SuperSelco are a good food for postlarval mahimahi. Yolksac larvae of mahimahi are an even better food, promoting faster growth at less cost, for large scale mahimahi aquaculturists. Different batches of yolksac mahimahi larvae varied by a factor of 10 in their concentration of DHA, but always had the highest level of DHA as much as 40% of total fatty acids (FAs). These "high HUFA" batches of YSL also had the highest levels of EPA and total fatty acids. The data suggest that climate and broodstock age may have considerable influence on larval nutrition.  相似文献   

The structure of the first coordination shell in liquid water   总被引：1，自引：0，他引：1  

Wernet P  Nordlund D  Bergmann U  Cavalleri M  Odelius M  Ogasawara H  Näslund LA  Hirsch TK  Ojamäe L  Glatzel P  Pettersson LG  Nilsson A 《Science (New York, N.Y.)》2004,304(5673):995-999

X-ray absorption spectroscopy and x-ray Raman scattering were used to probe the molecular arrangement in the first coordination shell of liquid water. The local structure is characterized by comparison with bulk and surface of ordinary hexagonal ice Ih and with calculated spectra. Most molecules in liquid water are in two hydrogen-bonded configurations with one strong donor and one strong acceptor hydrogen bond in contrast to the four hydrogen-bonded tetrahedral structure in ice. Upon heating from 25 degrees C to 90 degrees C, 5 to 10% of the molecules change from tetrahedral environments to two hydrogen-bonded configurations. Our findings are consistent with neutron and x-ray diffraction data, and combining the results sets a strong limit for possible local structure distributions in liquid water. Serious discrepancies with structures based on current molecular dynamics simulations are observed.  相似文献   

Interaction between the helicase domain of the tobacco mosaic virus replicase and a tobacco arginine decarboxylase     

Takumi Shimizu  Yasuyuki Yamaji  Yoshitake Ogasawara  Koji Hamada  Keitaro Sakurai  Toshihiko Kobayashi  Takato Watanabe  Tadaaki Hibi 《Journal of General Plant Pathology》2004,70(6):353-358

In a yeast two-hybrid screening test for tobacco proteins that interact with TMV replicase using the helicase (H) domain as bait, a cDNA clone was selected that encodes a polyamine biosynthetic enzyme, arginine decarboxylase (ADC). In yeast cells, the C-terminal internal region of ADC interacted with the H domain. This observation was confirmed in vitro by far-Western blotting. Inhibition of the binding between the H domain and the IRnHEL (I region and N-terminus of helicase domain) region by ADC using a yeast three-hybrid assay suggested possible interference of the heterodimerization of 126K and 183K by ADC.The nucleotide sequence data of pADCF reported in this study is available in the DDBJ/EMBL/GenBank databases under accession number AB110952  相似文献   

Prevalence of Salmonella spp. in pigs, chickens and ducks in the Mekong Delta, Vietnam   总被引：1，自引：0，他引：1  

Tran TP  Ly TL  Nguyen TT  Akiba M  Ogasawara N  Shinoda D  Okatani TA  Hayashidani H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):1011-1014

In order to determine the prevalence of Salmonella spp. in domestic animals in 6 provinces of the Mekong Delta, Vietnam, 1,098 fecal or intestinal content samples from pigs, chickens, and ducks were examined in the period from July to October, 2000. Salmonella spp. were isolated from 78 (7.1%) of the total samples, which included 23 (5.2%) of 439 pigs, 24 (7.9%) of 302 chickens, and 31 (8.7%) of 357 ducks. From those samples, 80 Salmonella strains were isolated and 25 serovars were identified. The predominant serovars were S. Javiana, S. Derby, and S. Weltevreden. S. Javiana and S. Weltevreden were detected together in pigs, chickens, and ducks. These results indicate that the serovars of Salmonella are widely distributed in domestic animals in the Mekong Delta, Vietnam.  相似文献   

Effect of recombinant canine interleukin-6 and interleukin-8 on tissue factor procoagulant activity in canine peripheral blood mononuclear cells and purified canine monocytes     

Ogasawara S  Daddona JL  Trimpert J  Stokol T 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(3):325-335

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Localization of leptin and leptin receptor in the bovine adenohypophysis   总被引：1，自引：0，他引：1  

Ogasawara H  Ohwada S  Nagai Y  Taketa Y  Matsuzaki M  Tanaka S  Watanabe K  Aso H  Yamaguchi T 《Domestic animal endocrinology》2008,35(1):8-15

The present study was carried out to detail the cellular localization of leptin (Lep) and the leptin receptor (LepR) in the bovine adenohypophysis. Lep immunoreactivity (Lep-ir) was found in about 30% of adenohypophysial cells in the gland. Immunochemistry of Lep and specific hormones using serial sections revealed that Lep-ir was present in 60.4% of somatotrophs, 15.9% of gonadotrophs, 6.5% of mammotrophs, 6.5% of thyrotrophs and 2.4% of corticotrophs. Both the common short isoform (OBRa) and the long isoform (OBRb) of LepR mRNA were expressed in the bovine adenohypophysis. LepR immunoreactivity (LepR-ir) was found in only 2.8% of the adenohypophysial cells and over 50% of LepR-ir cells were gonadotrophs, in which most of the cells were distributed in the zona tuberalis. The findings on Lep and LepR in the adenohypophysial cells indicate that Lep may regulate gonadotroph function through autocrine/paracrine pathway in the bovine adenohypophysis.  相似文献   

Effect of CO2 enrichment on fruit growth and quality in Japanese pear (Pyrus serotina Reheder cv. Kosui)     

Junki Ito  Shigeki Hasegawa  Kounosuke Fujita  Shizuhiko Ogasawara  Tamio Fujiwara 《Soil Science and Plant Nutrition》2013,59(2):385-393

Six year-old Japanese pear (Pyrus seratina Reheder cv. Kosui) trees grafted on P. serotina cv. Nihonyamanashi were grown in containers filled with Granite Regosol under glasshouse conditions. At different stages of fruit growth, pear trees were exposed to an elevated CO₂ concentration (130 Pa CO₂ ) along with a control (35 Pa CO₂). For one group of plants, CO₂ enrichment was applied for 79 d from 52 d after full bloom (DAB) to fruit maturity (long-term CO₂ enrichment) and for another group the same treatment was applied for 35 d from 96 DAB to fruit maturity (short-term CO₂ enrichment). The effects of the elevated CO₂ concentration on vegetative growth, mineral contents, and fruit production and quality were examined. Long-term CO₂ enrichment enhanced vegetative growth, without any significant effect on the mineral contents in either flower bud or fruit except for a remarkable increase in the K content. Long-term CO₂ enrichment increased the fruit size and fresh weight, but had no significant effect on the fruit quality. On the other hand, the short-term CO₂ enrichment did not induce any significant change in the fruit size but increased the fruit sugar concentration. Along with the reduction of the sorbitol concentration in fruit, the fructose and sucrose concentrations increased and these changes occurred earlier at elevated CO₂ than at ambient CO₂ concentrations. From these results, we concluded that the effect of CO₂ enrichment on fruit growth varies depending upon the growth stages of fruit: during the initial and fruitlet stages when fruit expansion occurs, CO₂ enrichment increases the fruit size, whereas, during maturation when fruit expansion has slowed down and sugar accumulation in fruit is active, it increases the fruit sugar concentration.  相似文献   

Tolazoline as an antagonist of xylazine in cattle   总被引：1，自引：0，他引：1  

K Takase  Y Hikasa  S Ogasawara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(4):859-862

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Antagonistic effects of alpha-adrenoceptor blocking agents on reticuloruminal hypomotility induced by xylazine in cattle.   总被引：1，自引：1，他引：0       下载免费PDF全文

Y Hikasa  K Takase  S Emi    S Ogasawara 《Canadian journal of veterinary research》1988,52(4):411-415

The intravenous injection of a standard dose (0.05 mg/kg) of xylazine inhibited reticuloruminal motility in cattle. Pretreatment with adrenoceptor antagonists showing alpha 2-blocking activity, tolazoline (0.5 mg/kg) and yohimbine (0.2 mg/kg), antagonized the xylazine-induced reticuloruminal amotility. Tolazoline was more effective than yohimbine, since an antagonistic effect was not seen at 0.5 mg/kg yohimbine, and yohimbine at 0.2 mg/kg was less effective than tolazoline at 0.5 mg/kg. An adrenoceptor antagonist showing alpha 1-blocking activity, prazosin, did not prevent the inhibition of reticuloruminal motility by xylazine. The xylazine-induced reticuloruminal amotility was also not prevented by either a dopamine receptor antagonist, domperidone, or an opiate receptor antagonist, naloxone. These results suggest that xylazine inhibits bovine reticuloruminal motility through its activation of alpha 2-adrenoceptors, and show that tolazoline can be used as a specific antagonist of xylazine in studies of the alpha-adrenergic influence on reticuloruminal motility in cattle.  相似文献