Aquaculture of the sand star,Astropecten indicus Döderlein, 1888, as a step toward the sustainable aquaculture of harlequin shrimp,Hymenocera picta Dana, 1852     

Jarunan Pratoomyot  Thammasak Torcharoen  Pairach Thongra-ar  Andrew P. Shinn 《Journal of the World Aquaculture Society》2020,51(1):282-286

Ornamental harlequin shrimp, Hymenocera picta, are commonly maintained on live comet sea stars, Linckia multifora. In Thailand, both species are commonly collected from the wild, raising concerns regarding the sustainability of such practices. As a step toward the sustainable aquaculture of both, methods of their culture and nutrition were investigated. Under captive conditions, harlequin shrimp readily predate, and can be maintained upon the astropectinid sand star Astropecten indicus as an alternative live feed during periods when comet sea stars are unavailable. Wild A. indicus subsequently spawned multiple times shortly after their collection; the eggs (300 μm diameter) were transferred to static aquaria. Following the appearance of planktonic bipinnaria, larval sea stars were fed a 50:50 Chaetoceros calcitrans and Isochrysis galbana diet. Eleven days post-transfer, brachiolaria stages began to settle; stages were then fed concentrated I. galbana. When specimens measured 1–2 mm in diameter, they were transferred onto an in-house formulated Amphiprion diet (>50% protein, >16% lipid). Sea stars were graded regularly; they matured and spawned after 6 months. Populations were maintained for more than three successive generations, demonstrating their ready culture under captive conditions, representing the first harvestable aquaculture of a sea star as a step toward supporting the sustainable culture of a primary, marine species.  相似文献   

Detection of nifH Sequences in Sugarcane (Saccharum officinarum L.) and Pineapple (Ananas comosus [L.] Merr.)     

Shotaro Ando  Masahiro Goto  Sompong Meunchang  Praphai Thongra-ar  Toru Fujiwara  Hiroaki Hayashi  Tadakatsu Yoneyama 《Soil Science and Plant Nutrition》2005,51(2):303-308

To detect N₂-fixing bacteria in a plant without using culture methods, nifH gene segments were amplified with degenerate primers from DNA extracted from stems of sugarcane ( Saccharum officinarum L.) and leaves of pineapple ( Ananas comosus [L.] Merr.). Sequences of the nifH clones were homologous to those of bacteria in the genera Bradyrhizobium, Serratia , and Klebsiella. The bacteria with nifH sequences homologous to those of the three genera may be promising candidates for predominant endophytic diazotrophs in sugarcane and pineapple, because these sequences were commonly detected in samples from different host plants, which were collected at different locations. On the other hand, no nifH sequence related to Gluconacetobacter diazotrophicus , which is an endophytic N₂-fixing bacterium and had been previously isolated from sugarcane, was detected in sugarcane. This indicates the absence or the presence of few G. diazotrophicus in the stems of the sugarcane plants used in the current study.  相似文献