Activin receptor type IIB in rohu (Labeo rohita): molecular characterization,tissue distribution and immunohistochemical localization during different stages of gonadal maturation     

Patnaik  Siddhi  Sahoo  Lakshman  Mohanty  Mausumee  Bit  Amrita  Meher  Prem Kumar  Das  Sachidananda  Jayasankar  Pallipuram  Saha  Jatindra Nath  Das  Paramananda 《Fish physiology and biochemistry》2021,47(5):1353-1367

Fish Physiology and Biochemistry - Activin receptor type IIB (ActRIIB) is a transmembrane serine/threonine kinase receptor which plays a pivotal role in regulating the reproduction in vertebrates...  相似文献   

Standardization of large scale production of homologous live attenuated PPR vaccine in India     

Hegde R  Gomes AR  Byregowda SM  Hugar P  Giridhar P  Renukaprasad C 《Tropical animal health and production》2008,40(1):11-16

Live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the Indian Veterinary Research Institute, Muktheswar, India. Vero cells between 130–150 passages with six percent foetal calf serum were used for the production of vaccine. The cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic effect was 80%. The vaccine was freezedried in order to maintain the stability of the vaccine. Identity test and titration was performed and the vaccine titre was monitored to be minimum of 10⁵/100 doses. In-house sterility tests and quality control tests using experimental animals and small ruminants were performed. The vacuum and moisture content of the vaccine were also regulated to be within the normal limits.  相似文献   

Identification and characterization of SNPs in released,landrace and wild accessions of mungbean (Vigna radiata (L.) Wilczek) using whole genome re-sequencing     

Bangar  Pooja  Tyagi  Neetu  Tiwari  Bhavana  Kumar  Sanjay  Barman  Paramananda  Kumari  Ratna  Gaikwad  Ambika  Bhat  K. V.  Chaudhury  Ashok 《Journal of Crop Science and Biotechnology》2021,24(2):153-165

Journal of Crop Science and Biotechnology - Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] is vital grain legume having nutritional and socio-economic importance, especially in the...  相似文献   

Evaluation of genetic relationship among six Labeo species using randomly amplified polymorphic DNA (RAPD)   总被引：1，自引：0，他引：1  

Paramananda Das  Harendra Prasad  Prem Kumar Meher  Ashoktaru Barat  & Ranjit Kumar Jana 《Aquaculture Research》2005,36(6):564-569

Labeo species constitute an important group of fish with intense diversity and potential for commercial aquaculture in many Southeast Asian nations including the Indian subcontinent. The present investigation involves the comparative analysis of randomly amplified polymorphic DNA (RAPD) profiles of six Labeo species viz., L. bata (bata), L. calbasu (calbasu), L. dyocheilus (dyocheilus), L. fimbriatus (fimbriatus), L. gonius (gonius) and L. rohita (rohu) at the nuclear DNA variation level. Fifteen decamer random primers were chosen from 40, which amplified a total of 449 DNA fragments ranging in size from 400 to 3000 bp. Both monomorphic and polymorphic DNA bands were identified based on their presence or absence that could be used for specieswise differentiation. Similarity coefficients were calculated to quantify the genetic variation within and between species. On an average, the highest intra‐species genetic similarity value was found in calbasu (0.93) followed by rohu and fimbriatus (0.91), bata (0.87), gonius (0.86) and dyocheilus (0.77). The interspecies genetic similarity estimates among the species of Labeo were used to deduce their phylogenetic relationships. The cluster analysis showed two main clusters, one with calbasu, rohu, fimbriatus and gonius and another with bata and dyocheilus. The study provides evidence that RAPD could be used for genetic differentiation of closely related species.  相似文献   

Utility of cross‐species amplification of 34 rohu microsatellite loci in Labeo bata,and their transferability in six other species of the cyprinidae family     

Alok Patel  Paramananda Das  Ashoktaru Barat  Prem Kumar Meher  Pallipuram Jayasankar 《Aquaculture Research》2010,41(4):590-593

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Genetic divergence in Indian populations of M. rosenbergii using microsatellite markers          下载免费PDF全文

Padmanava Mohanty  Lakshman Sahoo  Bindu R Pillai  Pallipuram Jayasankar  Paramananda Das 《Aquaculture Research》2016,47(2):472-481

Macrobrachium rosenbergii, known as the giant freshwater prawn or Malaysian prawn, is the sixth largest aquaculture species in Asia. Knowledge of genetic diversity of M. rosenbergii is important to support management and conservation programmes, which will subsequently help in sustainable production of this economically important species. This study aimed to analyse the genetic diversity and population structure of five M. rosenbergii populations using 11 microsatellite loci. In analysing 240 samples, the number of alleles, observed heterozygosity (H_O) and expected heterozygosity (H_E) ranged from 3 to 20, from 0.250 to 0.978 and from 0.556 to 0.944 respectively. The five stocks of M. rosenbergii displayed high level of genetic diversity. Both the F_ST and amova analyses showed that there was significant genetic differentiation among all populations. The UPGMA dendrogram based on Nei's genetic distance matrix revealed that the Narmada and Mahi populations were in one cluster and Mahanadi and Subarnarekha populations in another single major branch, whereas the Kerala population clearly showed a separate cluster. This information on genetic variation will be useful for genetic improvement and conservation of Indian populations of giant freshwater prawn M. rosenbergii.  相似文献   

Identification and characterization of SSRs in <Emphasis Type="Italic">Clarias batrachus</Emphasis> and their application in population study     

Shreya Srivastava  Basdeo Kushwaha  Jyoti Prakash  Manmohan Pandey  Suyash Agarwal  Ravindra Kumar  N. S. Nagpure  Mahender Singh  Paramananda Das  Chaitanya Joshi  Joy Krushna Jena 《Fisheries Science》2017,83(2):265-272

Magur Clarias batrachus is an indigenous catfish, commonly found in India, Nepal, Bhutan and Bangladesh. Presently, the populations of magur have declined rapidly in their natural habitat mainly due to overexploitation and habitat degradation. Understanding the population genetic structure of the species has significance in improvement of stocks and in conservation of the species. In the present study, simple sequence repeat (SSR) markers were used to differentiate the populations of magur, collected from three geographic locations. For this, a total of 31,814 SSRs were mined from the de novo assembled pooled of whole genome sequence data of C. batrachus. A bioinformatics pipeline with stringent criteria was applied to analyze the data which resulted in selection of 30,142 microsatellite loci falling in the intergenic region. Out of the 25 loci selected for primer development, 16 loci were successfully amplified and 9 loci were found to be polymorphic in this species. The average observed as well as expected heterozygosity in the loci across different stocks varied from 0.652 to 0.688 and 0.864 to 0.873, respectively. These three populations were further segregated into two clusters based on the NJ genetic distance. The Lucknow population formed one cluster, while the Bhubaneswar and Kolkata populations constituted another cluster. A comparable finding was also deduced from the STRUCTURE analyses. The results revealed significant variation among the populations of C. batrachus under study.  相似文献