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Parishmita Handique Hemanta Pokhrel Binod Kalita Raktim Sarmah Abdul Malik Ahmed Kaustabh Bhagawati Arnab Narayan Patowary 《Aquaculture Research》2020,51(5):1973-1979
The present study was conducted to evaluate the …effect of dietary selenium on growth performance and antioxidant enzymes activity in Cirrhinus mrigala for a period of 45 days. Four numbers of experimental diets were prepared, Tc for Control (0 mg organic selenium/kg of diet), T1 (2.5 mg organic selenium/kg of diet), T2 (5.0 mg organic selenium/kg of diet) and T3 (10 mg organic selenium/kg of diet). The water quality parameters (temperature, DO, pH, conductivity, hardness, ammonia, nitrate and nitrite) were found to be insignificant (p > .05) among all the treatments. Superoxidase dismutase (SOD) was found to be significantly highest in T2 (6.10 ± 0.07) when compared with the other treatments. Similarly, catalase (CAT) activity in T1 (13.21 ± 0.13) was significantly (p < .05) different with that of Control, T2 and T3. Present study also revealed that 2.5mg organic selenium/kg supplemented diet (T1) showed highest results in net weight gain (15.55 ± 1.36 g), net weight gain percentage (185.88 ± 8.98%) and SGR (2.35 ± 0.06 g) compared with other experimental diet. 相似文献
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A. K. Handique 《Euphytica》1986,35(2):631-632
Summary In nature, Solanum khasianum
Clarke (syn. S. varium
Dunal) is a self pollinated plant. However, artificial cross pollination is feasible when pollination is done around the time of anthesis, which is between 09.30 and 10.00. Due to prevalence of heterostyly bud selection has to be highly specific. 相似文献
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RAPD and microsatellite markers were used to determine the genetic fidelity of micropropagated plants from the three varieties of tea plant derived from explants of field grown mother bush as well as in vitro germinated seedlings. Rate of shoot multiplication was better from nodal explants than from shoot tips. A maximum of 32–33 shoots was observed in cotyledonary node in 1/2 MS medium with BAP (6 mg/l), GA3 (1 mg/l) and IBA (0.5 mg/l). 90% of the in vitro derived microshoots were micrografted into rootstocks. The micropropagated plantlets showed both cytological and genetical stability. SSR primers showed complete stability among the regenerants. The results convinced that plants derived from axillary as well as adventitious mode of propagation can be genetically true to type. This cost effective technique would help in fast clonal propagation at a commercial scale. 相似文献
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Sumita Acharjee Pratap Jyoti Handique Bidyut Kumar Sarmah 《Journal of Crop Science and Biotechnology》2012,15(4):311-318
Regeneration has been achieved in blackgram (Vigna mungo) using thidiazuron (TDZ) in the culture medium. The explanted cotyledon with wounded embryonic axes produced the highest number (9.75–10.45) of healthy, elongated shoots when cultured on shoot bud regeneration medium (SRI) composed of 2 μM BAP, 2 μM KIN, 2 μM TDZ, and 0.5 μM NAA followed by multiple shoot regeneration (SRII) medium containing 2 μM BAP, 2 μM KIN, and multiple shoot elongation (SE) medium (0.5 μM of BAP + 0.5 μM of KIN). The presence of TDZ in combination with BAP and NAA in the SRI medium for one sub-culture cycle (10–14 days) significantly increases formation of multiple shoot buds per explant. Independent, healthy shoots obtained were selected for both in vitro rooting and grafting. Establishment of plantlets in the soil was highest (80–100%) in the case of in vitro rooted compared to grafted shoots (40%). The protocol appears to be competent to Agrobacterium-meditated transformation with ‘gus’ as a reporter gene. PCR analysis of the T0 and T1 progenies showed the presence and transmission of the transgene. We document here the regeneration and transformation of blackgram using cotyledons with wounded embryonic axes and the protocol appears to be suitable for genetic transformation of blackgram. 相似文献
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