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Asma Ben Ghnaya Mohsen Hanana Ismail Amri Hazar Balti Samia Gargouri Bassem Jamoussi Lamia Hamrouni 《Journal of pest science》2013,86(3):571-577
The chemical composition of essential oil isolated by hydrodistillation from the leaves of Tunisian Eucalyptus erythrocorys Linn. was analyzed by gas chromatography (GC) and gas chromatography–mass spectrometry (GC/MS). Fifteen compounds were identified, representing 97 % of total oil, which was found to be rich in oxygenated monoterpenes (66.7 %), particularly in 1,8-cineole (54.8 %). The yield and the physico-chemical properties of oil were determined. The study of antifungal activity revealed that E. erythrocorys essential oil significantly inhibited the growth of five plant pathogenic fungi especially Bipolaris sorikiniana and Botritys cinerea. Moreover, herbicidal properties of the oil, tested on Sinapis arvensis L. and Phalaris canariensis L. indicated that the E. erythrocorys oil completely inhibited the seed germination at high concentration (1.5 μl/ml), while at low doses (0.25, 0.5, 0.75, 1 μl/ml) the oil acted by decreasing and delaying the germination and inhibiting the seedling growth of all tested weeds. 相似文献
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Ahmed Barkia Ali Bougatef Rim Nasri Emna Fetoui Rafik Balti Moncef Nasri 《Fish physiology and biochemistry》2010,36(4):893-902
Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose
anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the
purified enzyme was estimated to be 23 kDa by SDS–PAGE and size exclusion chromatography. The purified trypsin appeared as
a single band on native-PAGE and zymography staining. The purified enzyme showed esterase-specific activity on N-α-benzoyl-l-arginine ethyl ester (BAEE) and amidase activity on N-α-benzoyl-dl-arginine-p-nitroanilide (BAPNA). The optimum pH and temperature for the enzyme activity, after 10 min incubation, were pH 9.0 and 55°C,
respectively, using BAPNA as a substrate. The trypsin kinetic constants K
m and k
cat on BAPNA were 0.13 mM and 1.56 s−1, respectively, while the catalytic efficiency k
cat
/K
m was 12 s−1 mM−1. Biochemical characterisation of B. boops trypsin showed that this enzyme can be used as a possible biotechnological tool in the fish processing and food industries. 相似文献
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Naourez Ktari Nahed Fakhfakh Rafik Balti Hayet Ben Khaled Moncef Nasri 《Journal Of Aquatic Food Product Technology》2013,22(5):436-448
We have investigated the antioxidant activities of eight hydrolysates from cuttlefish by-products obtained by treatment with various gastrointestinal proteases (chymotrypsin, trypsin, and crude alkaline enzyme extracts from cuttlefish and sardinelle) and bacterial proteases (Alcalase and crude enzymes from Bacillus pumilus A1, Bacillus mojavensis A21, and Bacillus cereus BG1). The antioxidant activities of the cuttlefish by-products protein hydrolysates (CPHs) were evaluated using various in vitro antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, and total antioxidant capacity. All hydrolysates showed different degrees of hydrolysis (DH) and varying degrees of antioxidant activity. Among the different hydrolysates, cuttlefish crude enzyme hydrolysate exhibited the highest antioxidant activity, followed by sardinelle crude enzyme and Alcalase hydrolysates. Further, CPHs with different degrees of hydrolysis were prepared by treatment with proteolytic enzymes from cuttlefish, sardinelle, and B. mojavensis A21. All hydrolysates showed a greater antioxidative activity as indicated by all the methods considered. In addition, antioxidant activity in hydrolysates was positively correlated with the increase of DH. The results of this study indicated that CPHs might be a good candidate for further investigation in developing new antioxidants. 相似文献
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