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Independent geological and geophysical investigations of the Mid-Ocean Ridge system have begun to focus on the nature of the magma chamber system underlying its central axis. Thermal models predict the existence of a steady-state chamber beneath a thin crustal lid ranging in thickness from 2 to 13 kilometers. The only aspect of the system that these models fail to account for is the extremely slow spreading rates. Seismological studies reveal the existence of a low-velocity zone beneath segments of the East Pacific Rise, which is thought to correspond to a chamber system having a half-width of approximately 5 to 10 kilometers. These estimates compare favorably with those derived separately through petrological investigations of deep-sea drilling results, various sampling programs, and field and laboratory studies of ophiolites. The chamber is thought to be wing-shaped and to remain continuously open; it is thought to be fed from the center while simultaneously solidifying at the sides as spreading carries the two halves apart. Progressive fractionation occurs by crystal settling coupled with repeated replenishment and magma mixing in an open steady-state system. Near-bottom studies reveal that the zone of extrusion above the chamber is narrow, but its eruptive history is cyclic in nature, in conflict with the predictions of a steady-state model. On-bottom gravity data at 21 degrees N on the East Pacific Rise reveal a negative gravity anomaly that may be related to the uppermost part of the chamber. The anomaly is only 2 kilometers wide and 1 kilometer below the sea floor. This feature may be associated with a short-term upper magma reservoir. The cyclic volcanic activity is directly related to the active phase of hydrothermal circulation responsible for the observed negative thermal anomaly. The volume of water associated with this circulation is equal to the entire ocean volume passing through the accretion zone approximately every 8 million years. This is about 0.5 percent of the world's rivers, but the effective transport rates of elements are comparable to those of rivers in that anomalies for individual elements are frequently between 100 and 1000 times the average river composition. The degree of subsurface dilution determines the final exit temperature and composition of the hydrothermal fluids, ranging from manganese domination at extreme dilution to iron at intermediate levels to sulfide deposition when low dilution occurs. The discovery of massive sulfide deposits on the East Pacific Rise is destined to have a profound impact on our understanding of ore-forming processes. Whether it will have any economic significance remains to be seen.  相似文献   
2.
Pectenotoxins from marine dinoflagellates of the genus Dinophysis are rapidly hydrolyzed by many shellfish to give pectenotoxin-2 seco acid, which isomerizes to 7-epi-pectenotoxin-2 seco acid. Three series of fatty acid esters of pectenotoxin-2 seco acid (PTX-2 seco acid) and 7-epi-PTX-2 seco acid were detected by LC-MS analysis of extracts from blue mussels (Mytilus edulis) from Ireland. The locations of the fatty acid ester linkages were identified by a combination of LC-MSn in positive- and negative-ion modes, LC-MS analysis of the products from reaction of the esters with sodium periodate, and NMR analysis of purified samples of the two most abundant ester derivatives. The 37-O-acyl esters of PTX-2 seco acid were the most abundant, followed by the corresponding 11-O-acyl esters, accompanied by low levels of the 33-O-acyl esters. The most abundant fatty acid esters in the fractionated sample were, in order, the 16:0, 22:6, 14:0, 16:1, 18:4, and 20:5 fatty acids, although a wide array of other PTX-2 seco acid fatty acid esters were also present at low levels.  相似文献   
3.
Microcystins are cyclic peptides produced by cyanobacteria, which can be harmful to humans and animals when ingested. Eight of the (more than) 90 microcystin variants presently characterized, contain the amino acid tryptophan. The well-researched oxidation products of tryptophan; kynurenine, oxindolylalanine, and N-formylkynurenine, have been previously identified in intact polypeptides but microcystin congeners containing oxidized tryptophan moieties have not been reported. Liquid chromatography-tandem mass spectrometric analysis of an extract of Microcystis CAWBG11 led to the tentative identification of two new tryptophan-containing microcystins (MC‑WAba and MC-WL), as well as eight other microcystin analogs containing kynurenine, oxindolylalanine and N‑formylkynurenine (Nfk). Investigation of one of these congeners (MC‑NfkA) by nuclear magnetic resonance spectroscopy was used to verify the presence of Nfk in the microcystin. Liquid chromatography-mass spectrometry analysis of a tryptophan oxidation experiment demonstrated that tryptophan-containing microcystins could be converted into oxidized tryptophan analogs and that low levels of oxidized tryptophan congeners were present intracellularly in CAWBG11. MC-NfkR and MC-LNfk were detected in standards of MC-WR and MC-LW, indicating that care during storage of tryptophan-containing microcystins is required.  相似文献   
4.
Genes for epilepsy mapped in the mouse   总被引:22,自引:0,他引:22  
The neurological mutant mouse strain E1 is a model for complex partial seizures in humans. The inheritance of epileptic seizures with seven conventional chromosomal markers and over 60 endogenous proviral markers was studied by means of back-crosses of E1 with two seizure-resistant strains, DBA/2J and ABP/LeJ. The major gene responsible for this epileptic phenotype (El-1) was localized to a region distal with respect to the centromere on chromosome 9. At least one other gene, El-2, linked to proviral markers on chromosome 2, also influences the seizure phenotype. In addition, a potential modifier of seizures was detected in the DBA/2J background. The location of El-1 on distal chromosome 9 may allow identification of an epilepsy candidate gene in humans on the basis of conserved synteny with human chromosome 3.  相似文献   
5.

We evaluated whether bearing tetrodotoxin (TTX) affects salinity stress in the juvenile tiger puffer Takifugu rubripes. Juveniles of hatchery-reared non-toxic T. rubripes [body weight (BW): 1.7?±?0.2 g, n?=?120] were divided into six tanks and acclimatized to salinity (8.5 ppt) that is equivalent to blood osmolality. Fish in three tanks were fed non-toxic diet, and those in the other three tanks were fed a TTX-containing diet (356 ng/g diet) three times a day until satiation. In each diet treatment, salinity of one tank was kept at 8.5 ppt, and the other two tanks were adjusted to either 1.7 or 34.0 ppt, and fish were reared for another 33 days. Then, we compared survival, growth, TTX accumulation, plasma osmolality, plasma cortisol, and glucose levels among treatments. We detected TTX only in the fish in the TTX-diet groups. Survival was highest at 8.5 ppt (70%) and lowest at 1.7 ppt in the TTX-diet group (20%). The BW was greater at 8.5 ppt, and plasma osmolality was significantly higher at 34.0 ppt than at any other salinities. Plasma cortisol level was significantly higher but glucose level was lower at 1.7 ppt. Possessing TTX at a low salinity may be lethal to tiger puffer juveniles.

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6.
The effect of adding carotenoids from various sources to feed of juvenile freshwater crayfish Cherax quadricarinatus on pigmentation, growth and survival was examined under laboratory conditions. Juvenile crayfish were fed diets fortified with carotenoids at a level of 100 μg g−1 for a period of either 49 or 55 days. Carotenoid sources were: dried algal cells prepared from Dunaliella salina in which the main carotenoid is β-carotene; a synthetic carotenoid, astaxanthin (Carophyll Pink 8%, manufactured by Hoffman–La Roche) and alfalfa meal. Crayfish receiving feeds fortified with carotenoids exhibited better body colouration than those in the control group, which were fed a diet to which no carotenoids were added. Growth and survival of the crayfish were not affected by the addition of carotenoids to their diet.  相似文献   
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