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This study was designed to investigate whether transcutaneous electrical nerve stimulation (TENS) of the longissimus dorsi muscle (MLD) of rabbits induces specific proportional changes in the muscle fatty acid composition. Ten 4-week-old Pannon White rabbits were exposed to TENS treatment two times a day, with the following settings: 30 Hz, 20 micros impulse length, 10 mA, 2 x 20 min. After a treatment period of 50 days rabbits were slaughtered and the fatty acid composition of the MLD was determined by gas chromatography. The TENS treatment increased the proportions of linoleic (C18:2 n-6), linolenic (C18:3 n-3) and gondoic acids (C20:1 n-9), compared with the control group. The level of palmitic (C16:0), stearic (C18:0), oleic (C18:1 n-9) and eicosapentaenoic (C20:5 n-3) acids significantly decreased. The proportion of total unsaturated fatty acids significantly increased. On the basis of the results obtained, TENS may have similar effects on the muscle fatty acid profile like physical training. Based on the supposal that the composition of membrane structure was also affected, the electrical stimulation of muscles may have further consequences, e.g. on membrane properties.  相似文献   
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Pannon White growing rabbits (a group of 8) were exposed to treadmill exercise (3-9 m/s, 1.2-1.6 km/day) twice a day for 4 weeks, while additional 8 animals, kept inactive, were assigned as the control group. Weekly, 12 hours after exercise, venous blood was taken for serum metabolite and enzyme activity measurements. Total serum protein, albumin and creatinine levels significantly increased during the second half of the training, as compared to the control group. Triacylglycerol levels in the exercised group as compared to controls, however, were higher only after the first and the fourth weeks of the experiment. Resting non-esterified fatty acid (NEFA) concentration of the trained rabbits was lower at the end of the trial. On the other hand, there were no significant differences, as compared to the respective controls, in serum urea, total and HDL cholesterol levels. At the end of the exercise alkaline phosphatase activity was higher and total lactate dehydrogenase activity was lower in the trained rabbits. Serum alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase activities were not changed, while creatine kinase activity was slightly lower in the trained group. The serum cortisol concentration was not different in the trained and control rabbits.  相似文献   
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The belly region composition of a representative commercial pig sample of 130 castrates was examined by in vivo spiral computed tomographic (CT) scanning. The lean meat percentage, the muscle/fat tissue ratio within the total body and separately within the bacon part were estimated by image analysis. The lean meat content of the total body was determined by the EU reference method. A correlation of r = 0.97 was found between lean meat content of the total body determined by CT estimation and the slaughtering results. The pigs were divided into seven groups on the basis of their lean meat content. The muscle percentage of the bacon part was 26% and 67%, respectively, in the two groups representing the two extremes of lean meat percentage (36% and 67%, respectively). The total volume of the bacon part was relatively constant (8.6 +/- 1 dm3) in the groups representing different lean meat categories. At the same time, the fat tissue volume of the bacon part was considerably higher while its muscle tissue volume was markedly lower in the group of the lowest than in that of the highest lean meat content (6.6 vs. 2.5 dm3 and 2.4 vs. 5.5 dm3, respectively). To describe changes in the tissue composition of the bacon part during fattening, 10 castrates were scanned repeatedly at 70, 90 and 110 kg of liveweight. A linear regression model was developed to characterise the continuous decrease observed in the muscle/fat ratio within the bacon part due to increasing liveweight.  相似文献   
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Sixty left sides of pig carcasses were scanned by spiral computed tomography (CT) to measure lean meat weight and percentage. The carcasses were fully dissected and scanned to develop a calibration protocol. Different image analyses were performed on the basis of anatomically defined scans, direct volumetric estimation, body- and grey-scale ranges and using Partial Least Squares (PLS) regression of data provided by CT. The R2 values of the calibrations for lean meat weight were 0.874, 0.976, 0.983 and 0.992, respectively, depending on the method applied. The PLS proved to be the best approach with a calibration RSD of 232 g. When changing from lean meat weight to percentage, the statistical goodness drops-to a very small extent (R2 = 0.988, RSD = 0.56). According to the results, the CT method can be recommended as a reference for determining the lean meat content of pig carcasses.  相似文献   
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A two-way selection procedure was performed on a Landrace pig line. First the cardiac output (CO) values (6.89 +/- 1.27 L/min) were estimated by ECG-gated magnetic resonance imaging (MRI) to quantify the heart performance of the boars (n = 30), then total body skeletal muscle volume (MVTB; 48.7 +/- 5.17 dm3) was determined applying spiral computerised tomography (CT). The relative cardiac output value (RCO) was developed, expressing the relationship between CO and MVTB. Four superior [RCO value below 6.7 dm3 / (L x min(-1))] and one minus variant [(8.97 dm3 / (L x min(-1))] boars were taken into breeding. The best fifteen percentage of the first generation males (n = 30) was characterised according to their RCO values. Significant difference was found between the developed performance measure of the progenies of minus and plus variant boars [8.2 +/- 0.65 and 5.63 +/- 0.70 dm3 / (L x min(-1)), n = 7 and 23], respectively. Comparing the existing five progeny groups, all of the 'HP+' groups differed significantly from the 'HP-' one, where the largest heart performance contrast found was 3.2 dm3 / (L x min(-1)). Based upon the results, the selection is being continued on a larger population.  相似文献   
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The sensitivity of ten Bordetella bronchiseptica and ten Pasteurella multocida strains, each isolated from cases of atrophic rhinitis (AR), was examined in tube dilution test. Getroxel, chlorquinaldol and oxytetracycline and the former two ones combined with trimethoprim inhibited the growth of both species in vitro. The minimum inhibitory and the minimum bactericidal concentration was less than 0.5 microgram/ml. When efficacy was tested in SPF in the group fed a combination of Getroxel, chlorquinaldol and oxytetracycline (60 mg, 240 mg and 360 mg/kg of feed, respectively), P. multocida disappeared from the nasal cavity by the end of a 30-day treatment. B. bronchiseptica was reisolated in low numbers from 2 out of 9 piglets. The daily body mass gain was by 7.9% higher and the feed conversion rate was by 19% better than in the control group. After slaughter, only mild signs of AR were seen in 3 out of 9 piglets treated with the above-mentioned drug combination, while in the control group severe lesions were observed in 8 out of 9 pigs. In treated commercial herds P. multocida disappeared from the nasal cavity of the piglets by the end of the treatment (42nd day of life), but the B. bronchiseptica strains could not be completely eliminated. Due to the treatment, mortality between 2 and 6 weeks of age decreased by 0.8-7.6%. Daily body mass gain was, on the average, 16.4% higher, the amount of feed needed for 1 kg body mass gain was by 15.3% lower and the duration of fattening was by 30.8 days shorter than in the control groups.  相似文献   
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