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Chatter R Ben Othman R Rabhi S Kladi M Tarhouni S Vagias C Roussis V Guizani-Tabbane L Kharrat R 《Marine drugs》2011,9(7):1293-1306
Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 μM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect. 相似文献
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Denoeud F Henriet S Mungpakdee S Aury JM Da Silva C Brinkmann H Mikhaleva J Olsen LC Jubin C Cañestro C Bouquet JM Danks G Poulain J Campsteijn C Adamski M Cross I Yadetie F Muffato M Louis A Butcher S Tsagkogeorga G Konrad A Singh S Jensen MF Cong EH Eikeseth-Otteraa H Noel B Anthouard V Porcel BM Kachouri-Lafond R Nishino A Ugolini M Chourrout P Nishida H Aasland R Huzurbazar S Westhof E Delsuc F Lehrach H Reinhardt R Weissenbach J Roy SW Artiguenave F Postlethwait JH Manak JR Thompson EM 《Science (New York, N.Y.)》2010,330(6009):1381-1385
Genomes of animals as different as sponges and humans show conservation of global architecture. Here we show that multiple genomic features including transposon diversity, developmental gene repertoire, physical gene order, and intron-exon organization are shattered in the tunicate Oikopleura, belonging to the sister group of vertebrates and retaining chordate morphology. Ancestral architecture of animal genomes can be deeply modified and may therefore be largely nonadaptive. This rapidly evolving animal lineage thus offers unique perspectives on the level of genome plasticity. It also illuminates issues as fundamental as the mechanisms of intron gain. 相似文献
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In this work, water-extraction of Vitis Vinifera L. (Black Grenache) leaves and analysis of aqueous extracts for anthocyanins were investigated. Vegetable leaves were cultivated in North of Tunisia at different dates. Anthocyanins were identified as main constituents in these natural dyeing materials using high performance liquid chromatography (HPCL). Dyeability of wool and its fastness properties using Vitis Vinifera L. leaves extract were also determined. Aqueous extract of Vitis Vinifera L. leaves has markedly yielded shades with good fastness properties. Thus, affordability is a point favorable for Vitis Vinifera L. leaves. Effects of dye bath pH and temperature on dyeability and fastness proprieties of wool fabrics by aqueous extract of Vitis Vinifera L. leaves were evaluated. Experimental results showed that fastness properties of dyed fabrics ranged from average to very good. Vitis Vinifera L. leaves extracts showed potential to dyeing wool fibers under highly acidic conditions (pH ≤2). The results obtained here revealed that natural dyes extracted from Vitis Vinifera L. leaves can be cost-effective for dyeing wool fabrics. 相似文献
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Ben Farhat Mouna Chaouch-Hamada Rym Sotomayor Jose A. Landoulsi Ahmed Jordán María J. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(1):15-20
Changes in the contents and composition of polyphenolics and resulting antioxidant activities of S. verbenaca by-products were investigated at three phenological stages (flowering, early fruiting and late fruiting stages). The highest accumulation of total phenolics was detected at the flowering stage (58.36 mg GAE/g DW). HPLC analysis of methanolic extracts showed the prevalence of methyl carnosate (821.45–919.82 μg/g DW) and rosmarinic acid (544.51–649.26 μg/g DW). Phenolic diterpenes (1056.90–1148.42 μg/g DW) was the most represented class of compounds. Three complementary tests namely, DPPH? (IC50 value, 49.22 μg/mL) and ABTS?+-radical scavenging assays (146.86 μM TE/mg) and FRAP reducing power test (188.93 mM Fe(II)/mg) were used to evaluate the antioxidant capacity and showed the best performance at the early fruiting period. The current study evidenced the significant effect of phenophase on antioxidants and contributed to valorize S. verbenaca extracts as a source of functional phenolic compounds.
相似文献5.
Gharsa H Ben Slama K Lozano C Gómez-Sanz E Klibi N Ben Sallem R Gómez P Zarazaga M Boudabous A Torres C 《Veterinary microbiology》2012,156(3-4):367-373
Nasal swabs of 163 healthy sheep were obtained from two farms and one abattoir in Tunisia during 2010. Samples were inoculated in Baird Parker agar and ORSAB medium for Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) recovery, respectively. MRSA was detected in 5 of these 163 samples (3%) in ORSAB medium, and one isolate per sample was further studied. MRSA isolates were mecA-positive, typed as ST153-CC80-t044-agrIII, and contained blaZ, ant(6)-Ia, aph(3')-IIIa, erm(C), tet(K), and fusB genes encoding penicillin, streptomycin, kanamycin, erythromycin, tetracycline and fusidic acid resistance, respectively. These MRSA isolates showed indistinguishable or closely related PFGE-patterns and harboured the lukF/lukS gene encoding the Panton-Valentine leukocidin and the luk-ED, hla, hld, and hlg(v) genes. Methicillin-susceptible S. aureus (MSSA) were recovered in 68 of the 163 samples (41.7%) and one isolate per sample was characterized. Most of MSSA (82.4%) showed susceptibility to the tested antibiotics with exceptions: penicillin (6%, carrying blaZ gene), tetracycline (19%, carrying tet(K) gene) and fusidic acid (9%). The following toxin-genes were identified among MSSA: tst (53 isolates), luk-M (52), luk-ED, hla, hlb, hld and hlg(v) (67), hlg (1), sec (49), sel (52), and the egc-cluster-like sen-sem-sei-seo-seg (1). Ten spa-types (two of them new ones) and nine sequence types (six new ones) were detected among the 73 S. aureus isolates, and they were ascribed to agr types I and III. All MRSA and MSSA isolates were able to coagulate bovine plasma and MRSA harboured the immune-evasion-gene-cluster type E. Conclusions. Nares of healthy sheep could be a reservoir of PVL-positive community-associated-MRSA and also of TSST-positive S. aureus isolates, with potential implications in public health. 相似文献
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Rym Trimech Yosr Zaouali Abdennacer Boulila Lotfi Chabchoub Ismail Ghezal Mohamed Boussaid 《Genetic Resources and Crop Evolution》2013,60(5):1621-1628
The morphological variation and the systematical status of 28 traditional melon including two ancient varieties collected from different geographical and bioclimatic areas of Tunisia were assessed based on 34 qualitative and quantitative traits. Yellow Canary was used as a reference variety. Significant differences among accessions within and among sites of collection were revealed for the majority of traits. The accessions from the Sahel were more polymorphic than those from Tozeur’s oasis. However, the classification of the local accessions according to Munger and Robinson’s varietal groups did not allow clear segregation of several of them because of their high heterogeneity. The principal component analysis (PCA) performed on all measured traits, allowed a distinction among the introduced varieties clustered according to their varietal group, and the local accessions grouped separately according to their geographical origin and bioclimatic zone. Mahalanobis distances among PCA’s groups were significant. The correlation found between Mahalanobis distance matrix and geographical distance matrix among origins corroborated isolation per distance. Conservation strategies should be made appropriately according to origins. Accessions from the Sahel showing the highest polymorphism within and among sites of collection should be firstly preserved. 相似文献
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This paper explores the potential uses of the aqueous extract of Vitis vinifera L. leaves in dyeing linen and silk fabrics without a metal mordant. The focus will be on investigating the tinctorial potential of Vitis vinifera L. leaves and its dyeing properties. The anthocyanins amounts of leaves from different Vitis vinifera L. varieties were determined by the spectrophotometry method. The effect of the main dyeing parameters (pH of the dye bath, temperature, and dyeing duration) on the dyeing quality results has been studied. Tannic acid and pomegranate peel extract have been used as a natural mordant. Experimental results have shown that fastness properties of dyed fabrics ranged from average to very good. 相似文献
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Rym Zitari-Chatti Noureddine Chatti Ali Elouaer & Khaled Said 《Aquaculture Research》2008,39(1):70-76
Ten populations of Penaeus kerathurus (Forskäl), a prawn of high commercial value, were sampled from the eastern and western Mediterranean coastal waters of Tunisia and screened electrophoretically for genetic variation at 13 allozyme loci. Four among the six polymorphic loci were out of Hardy–Weinberg equilibrium (H–WE) in at least one population. In the same way, the multilocus test showed deviation from H–WE in six populations. These populations showed heterozygote deficiency whereas the average heterozygosity for the four remaining ones is quite similar to the expected levels. Genetic variability was low. The number of alleles per locus ranged from 1.2 to 1.5 (average=1.3), and the observed heterozygosity varied between 0.010 and 0.048 (average=0.021). Significant population differentiation (FST=0.076, P<0.05) in the total data set reflected the differentiation of the two populations, which were at the margins of the range sampled, from all the others (Pairwise FST values ranged from 0.035 to 0.208). Although there was no significant differentiation among the other populations (pairwise FST values ranged from ?0.006 to 0.201, P>0.05). Our data suggest a population structure consistent with separation by Mediterranean Sea basins that might reflect different local biogeographical zones. 相似文献
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