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Ryoma Kamikawa Isao Masuda Kenichi Oyama Sadaaki Yoshimatsu Yoshihiko Sako 《Fisheries Science》2007,73(4):871-880
In this study, nuclear ribosomal RNA gene internal transcribed spacer regions and the cox2-cox1 fragment of the mitochondrial (mt) genome were sequenced in 24 strains of Chattonella spp. Variability in both regions showed that the mt genome sequences of Chattonella spp. have a higher evolutionary rate than the nuclear rRNA gene sequences. A maximum likelihood tree based on the mt sequence
grouped the Japanese Chattonella strains into two groups (Groups A and B), although no correlation was observed amongst the phylogenetic groups, their morphologies,
or the isolated areas. Groups A and B were clearly identified by a polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) assay using Fokl, without the need for a sequencing experiment. The PCR-RFLP assay revealed that Chattonella cells obtained from sea water in Oita, Japan, in 2004 and 2005 belonged to Group B. This is the first report showing the
genetic variation in Chattonella spp. using a PCR-RFLP identification protocol. 相似文献
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Anisaldehyde (p-methoxybenzaldehyde) was previously reported to inhibit the tyrosinase-catalyzed oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) noncompetitively as long as the enzyme activity was monitored by measuring dopachrome formation. However, anisaldehyde did not inhibit this oxidation if a longer reaction time was observed, although it suppressed the initial rate of oxidation to a certain extent. Anisaldehyde significantly suppressed the rate of enzymatic oxidation of L-tyrosine. 相似文献
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