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Do Thi Thanh HUONG Vidya JAYASANKAR Safiah JASMANI Hisako SAIDO-SAKANAKA Andrew J. WIGGINTON Marcy N. WILDER 《Fisheries Science》2004,70(3):518-520
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ABSTRACT: In the present study, the role of carbonic anhydrase (CA) and Na/K-ATPase in the gill and epidermal tissues in the giant freshwater prawn Macrobrachium rosenbergii was examined as a function of molting stage. CA activity levels in the front and back gills were low at the intermolt stage C0 , but increased significantly at premolt stage D3 , and then decreased after molting. In the epidermal tissue, activity levels decreased gradually towards premolt to a minimum level at stage D3 , but became elevated at postmolt stages A and B. Na/K-ATPase levels in the front and back gills did not change significantly during the molt cycle. CA in the gill is possibly involved in supplying counter-ions for ion uptake, while CA in the epidermal tissue may play a role in mineralizing the exoskeleton after ecdysis. Na/K-ATPase in the gills may function in ion uptake from the ambient medium; however, since its activity was not influenced by the molt cycle, it probably does not have a major role in osmoregulation in the freshwater environment. 相似文献
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Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn,Macrobrachium rosenbergii 
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下载免费PDF全文 Using Hsp70 as a biomarker, thermal stress impinges on reproductive organs, ovary and hepatopancreas were being analyzed by determining the expression of Hsp70 mRNA inside the organs after the adult inter‐molt females were subjected to thermal treatment at 35, 30 and 28°C (Control). Results showed the expression of Hsp70 mRNA under thermal treatment of 35°C after 2 hr recovery in ovary were upregulated at 2, 4, 6, 12, 24 hr and 30 days compared to control whereas in hepatopancreas under similar treatment, the expression of Hsp70 mRNA were significantly higher than control at 6, 24 hr and 30 days. Frequency of reproductive molt at 35°C showed the ovary of females were failed to develop and only entered common molt along three consecutive molt cycles. For 30°C thermal treatment, the expression of Hsp70 mRNA was significantly higher than control after 2 hr recovery but returned to normal afterwards until 30 days’ thermal treatment. Maternal heat shock for 2 hr at 35°C were found to give significantly lower frequency of reproductive molt and longer duration of ovarian development and incubation period whereas maternal heat shock for 2 hr at 30°C gave lower frequency of reproductive molt, slower development of embryo and lower hatching success compared to untreated control. This study suggests that short and long‐term thermal stress at 30 and 35°C were found to affect the induction of Hsp70 mRNA in reproductive organs of Macrobrachium rosenbergii and also influence their reproductive performance. 相似文献
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Changes in hemolymph osmolality, ion concentrations, and enzymatic activities of carbonic anhydrase (CA) in the gills and
epidermal tissue, and Na/K-ATPase in the gills during the molt cycle were investigated in the white shrimp Litopenaeus vannamei. Hemolymph osmolality was high in the intermolt and early premolt stages, but started to decrease prior to ecdysis through
to postmolt stages A and B. Changes in Na+ and Cl− ion concentrations paralleled those in hemolymph osmolality. CA activity levels in the anterior and posterior gills were
low at intermolt stage C0 and premolt stage D0, and maximum at premolt stage D3. In the epidermal tissue, activity was relatively high at intermolt stage C0 and premolt stage D0, but fluctuated towards premolt stage D3 and postmolt stage A. On the other hand, Na/K-ATPase activity in the gills decreased between intermolt stage C0 and premolt stage D2, but increased at premolt stage D3 and postmolt stage A. The changes in patterns of CA activity during the molt cycle suggest that CA may be involved in supplying
counter-ions for Na+ and Cl− uptake during molting. Branchial Na/K-ATPase appears to be involved in producing local osmotic gradients in order to support
water influx across the epithelium. 相似文献
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Ahmad Daud Om Safiah Jasmani Nosrihah Ismail S. Y. Yeong A. B. Abol-Munafi 《Fish physiology and biochemistry》2013,39(5):1277-1286
A new proteomics technology has been implemented to study the protein repertoires of developing oocytes of giant grouper (Epinephelus lanceolatus). Knowledge of the chemical composition and physiochemical properties of vitellogenin (Vtg) is necessary to interpret the functional and biological properties attributed during ovulation. Vtg, as a biomarker indicator in sex determination, has been analyzed to determine the sex and maturational status of fish in the absence of the gonad tissue. A male giant grouper was induced by 2 mg/kg of 17ß-estradiol (E2), and blood was sampled at days 0, 1, 3, 5, and 10. SDS-PAGE 1D electrophoresis was used to analyze Vtg protein, and Vtg identification was done with 4800 Plus MALDI TOF/TOF? mass spectrophotometer (Applied Biosystems/MDS SCIEX, USA). Meanwhile, MS/MS de novo sequencing identified the proteins by matching sequences of tryptic peptides to the known sequences of other species. Vtg was confirmed by MASCOT at 95 % significant level, and molecular mass was 187 kDa. Protein resolved on SDS-PAGE as a double band of approximately the same mass as determined with MALDI-TOF. The N-terminal sequences and identification of Vtg were also determined. The potential of using MS methods to understand the structure and function of Vtg is discussed. 相似文献
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Dynamics of glucose in the haemolymph of female giant freshwater prawn,Macrobrachium rosenbergii,influences reproductive and non‐reproductive moulting cycles 下载免费PDF全文
下载免费PDF全文 Noor Azlina Kamaruding Noraznawati Ismail Safiah Jasmani Marcy N Wilder Mhd Ikhwanuddin 《Aquaculture Research》2017,48(7):3505-3514
Glucose, when measured in haemolymph, has been found to reflect a useful predictor of energetic investment. This study evaluated the pattern of glucose in the haemolymph, with an attempt to gain a better insight into the role of glucose as nutritional source of ovarian development and energy reserves during reproductive and non‐reproductive moulting cycles. The haemolymph of female giant freshwater prawn, Macrobrachium rosenbergii, was obtained at eight different moulting stages, and levels of glucose were determined using an enzymatic colorimetric glucose‐oxidase method in parallel with a histological examination of ovarian development. Glucose levels were relatively low (0.15 ± 0.02 mg mL?1) at D0 stage, an abrupt increase (0.52 ± 0.13 mg mL?1) during premoult D1 stage and declined (0.32 ± 0.10 and 0.31 ± 0.09 mg mL?1) during premoult D2 and D3 stages, respectively; thereafter, a slight increase (0.43 ± 0.09 mg mL?1) occurred at post‐moult A stage. The progression of ovarian growth, marked by an increasing gonadosomatic index (GSI) pattern during the reproductive moulting cycle (C0–D3 stages), was directly proportionate to fluctuations in glucose levels. GSI was significantly positively correlated with glucose (R = 0.40; P < 0.05). In contrast, glucose was notably higher at post‐moult A and premoult D2 stages during non‐reproductive moulting cycle, the period during which glucose is crucial for exoskeletal chitin synthesis. At this particular stage, a negative correlation between body weight and glucose (R = ?0.36; P < 0.05) was observed. The dynamics of glucose in the haemolymph of female M. rosenbergii correlated with ovarian growth, which signify that glucose as nutritional source for vitellogenesis, and affects the body weight of this species. 相似文献
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