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Acetaminophen (APAP)-induced liver damage is one of the most common problems among the population. Therefore, the study was aimed to investigate the hepatoprotective effect of celery leaves on APAP-induced toxicity in a freshwater fish, Pangasius sutchi. Fish were divided into four experimental groups of 6 fish each. Group 1 served as control. Group 2 fish were exposed to APAP (500 mg/kg) for 24 h. Groups 3 and 4 fish were exposed to APAP + celery leaf powder (CE) (500 mg/kg) and CE for 24 h, respectively. The severity of liver damage, hepatic lipid, glycogen, ions status and histological alterations was examined. The characterization of CE extract was also performed. APAP-exposed fish showed elevated levels of both circulating and tissue hepatotoxic markers (AST, ALT and ALP), reduced hepatic glycogen and lipid contents (TG and cholesterol), increased tissue lipid peroxidation markers (TBARS, LHP and PCO), altered tissue levels of enzymatic (SOD, CAT, GPx and GST) and non-enzymatic (GSH) antioxidants and cellular thiol levels (T-SH, P-SH and NP-SH), and reduced hepatic ions (Na+, K+ and Ca2+) and abnormal liver histology. The abnormalities associated with APAP exposure were reversed on treatment with CE. The TLC separation and HPLC quantification of petroleum ether/acetone extract of CE showed the peaks for highly efficient flavonoids such as rutein, quercetin and luteolin. The observed hepatoprotective effect of CE might be due to its rich flavonoids. 相似文献
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Akshaya Panigrahi Mani Sundaram Saranya Chakrapani Satishkumar Rajasekar Jagabattula Syama Dayal Gopal Chavali 《Aquaculture Research》2019,50(1):29-41
A 16‐week indoor culture trial was conducted to evaluate the effect of varying C:N ratio on growth performance, physico‐chemical parameters, microbial dynamics, feed utilization, and immunological parameters. The experiment comprised of five biofloc treatment groups (with varying C:N ratio 5:1, 10:1, 15:1, 20:1) and a control with three replicates each, having 100 nos/m3 as stocking density in 500 L tanks with constant aeration. The C:N ratios of the treatments were manipulated using molasses as an organic carbon source whereas there was no carbon source added in control. The water quality parameters monitored throughout the experiment were found to be within permissible limits in shrimp culture. At the end of the experiment, it was observed that there were significant differences between the treatment groups and the control regarding absolute growth, SGR, FCR, PER, and FER. Furthermore, a considerable difference in immunological parameters, namely, THC, phagocytosis, and PO activity (17.5 × 106 cells per ml, 43.5%, 0.112 Units min?1 mg min?1), was recorded among the treatments compared to that of the control groups (6.2 × 106 cells per ml, 31.5%, 0.051 Units min?1 mg min?1) respectively. Enhanced growth and survival with substantial disease resistance were recorded in C15 treatment. The results indicate that the CN15 ratio coupled with minimal water exchange is optimal for improved survival, growth, and immune activity. 相似文献
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T. P. Sathishkumar P. Navaneethakrishnan S. Shankar R. Rajasekar 《Fibers and Polymers》2014,15(9):1927-1934
Natural fiber composite replaces the conventional and synthetic materials in many fields especially in light weight applications. The randomly oriented short snake grass fiber reinforced isophthallic polyester composites are prepared by hand lay-up technique and finally compression molded. The various length and weight fraction of fiber are used in composite fabrication. The mechanical properties and water absorption under various climatic conditions are examined according to the prescribed standard. SEM image revealing the fiber pullout and breakage of the tensile and impact fractured composite specimens has been analysed and compared with control through scanning electron microscope. The result shows that the mechanical properties increase with increase in fiber length and weight fraction of the composites. The rate of water absorption increases with increase in temperature and time. Obtained experimental tensile strength of the composite is compared with various theoretical models such as Series, Hirsch’s, Halpin-Tsai, Modified Halpin-Tsai and Modified Bowyer & Brader’s and the obtained inferences are discussed. 相似文献
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Sudhakaran M Avudainayagam S Radha M Rajasekar M 《Communications in Soil Science and Plant Analysis》2020,51(13):1747-1756
ABSTRACT A field evaluation of the stable isotopes (δ13C and δ15N) and their relationship among physicochemical and enzyme activities was conducted in Indian semi-arid agricultural soils. Composite soil samples were collected based on organic management (ORG), inorganic management (IM), integrated crop management (ICM) and precision farming (PF) experimental plots from the fall of September 2017 to October 2018. δ15N was significantly higher (13.85 %) in ORG soils compared with ICM (13.28 %), IM (12.84 %) and PF (12.75 %). In contrast δ13C was higher (?13.25%) in PF soils than IM (?13.6 %), ICM (?15.07 %) and ORG (?15.23 %). Soils from ORG had significantly higher levels of total N, total C, total S, organic carbon, available N, extractable P, Soil organic carbon stock, exchangeable K and enzyme activities compared to IM, ICM and PF. Urease, β – glucosidase, acid phosphatase, alkaline phosphatase, invertase, cellulase and dehydrogenase activities significantly increased the δ15N and reduced δ13C in agricultural soils. Our results suggested that organic management had improved the δ15N, plant available nutrients and soil enzyme activities. Stable δ13C and δ15N isotopes are good indicators of monitor the soil health, carbon, and nitrogen biogeochemical cycles in Indian semi-arid agricultural soils. 相似文献
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Shanmugam Rajasekar Shui-zhang Fei Nick E. Christians 《Genetic Resources and Crop Evolution》2007,54(1):45-53
Colonial bentgrass (Agrostis capillaris L.) is a cool-season grass, native to temperate Asia and Europe. It has good tolerance to low temperatures and partial shade
and is well suited to golf course fairways and tees. Little information is available regarding levels and patterns of genetic
variation among populations of colonial bentgrass, which would be useful for breeding programs. To study the genetic relationships
among 27 colonial bentgrass accessions obtained from the US National Plant Germplasm System (NPGS), randomly amplified polymorphic
DNA (RAPD) markers were scored and analyzed. Out of 80 primers screened, 16 were selected for further analysis, which yielded
a total of 120 polymorphic bands used to differentiate the accessions. Dice's similarity coefficients for pair-wise comparisons
ranged from 0.23 to 0.84 based on the RAPD data. Since there was no similarity coefficient value close to 1 between any two
accessions, there was no apparent duplication among the sampled accessions. A dendrogram constructed on the basis of the Unweighted
Pair Group Method with Arithmetic average (UPGMA) clustering algorithm clearly separated 26 of the accessions into three clusters
with one accession distinct from the rest. The least similar pair of accessions was PI 204397 from Turkey and PI 628720 from
Bulgaria, and the most similar pair was PI 509437 from Romania and PI 491264 from Finland. Clustering patterns based on principal
components analysis (PCA) corresponded well with the dendrogram. A high cophenetic correlation (r = 0.82) was found between the RAPD data matrix and cophenetic matrix. The accession PI 628720, from Bulgaria, did not cluster
with any other accessions. 相似文献
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