全文获取类型
收费全文 | 1026篇 |
免费 | 52篇 |
国内免费 | 1篇 |
专业分类
林业 | 96篇 |
农学 | 35篇 |
157篇 | |
综合类 | 40篇 |
农作物 | 41篇 |
水产渔业 | 156篇 |
畜牧兽医 | 459篇 |
园艺 | 10篇 |
植物保护 | 85篇 |
出版年
2023年 | 2篇 |
2022年 | 18篇 |
2021年 | 45篇 |
2020年 | 27篇 |
2019年 | 14篇 |
2018年 | 27篇 |
2017年 | 25篇 |
2016年 | 47篇 |
2015年 | 34篇 |
2014年 | 56篇 |
2013年 | 85篇 |
2012年 | 59篇 |
2011年 | 63篇 |
2010年 | 33篇 |
2009年 | 40篇 |
2008年 | 67篇 |
2007年 | 72篇 |
2006年 | 77篇 |
2005年 | 63篇 |
2004年 | 53篇 |
2003年 | 33篇 |
2002年 | 42篇 |
2001年 | 21篇 |
2000年 | 16篇 |
1999年 | 5篇 |
1998年 | 12篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 7篇 |
1990年 | 8篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有1079条查询结果,搜索用时 15 毫秒
1.
2.
Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage
Masashi Ando Masao Joka Satoshi Mochizuki Koh-Ichi Satoh Yasuyuki Tsukamasa Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel. 相似文献
3.
K. Tagata S. Yokoyama T. Ginbo M. Honda T. Okimura M. Odakura M. Nomura S. Yamamoto 《Veterinary research communications》1996,20(1):21-30
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP
C-reactive protein
- ELISA
enzyme-linked immunosorbent assay
- RPLA
reversed passive latex agglutination test
- SRID
single radial immunodiffusion 相似文献
4.
Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(2):173-176
The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 相似文献
5.
AIM: To study the molecular biological mechanism and signal transduction pathway of interleukin-1β (IL-1β)-induced apoptosis in A375-S2 melanoma cells. METHODS: Photomicrocropy showed typical apoptotic changes. The cytotoxic effect of IL-1β in vitro and influences of caspases in this effect were measured by MTT assay. The cytotoxicity of cells was assessed by LDH-based assay. Degradation of DNA was detected by agarose gel electrophoresis. RESULTS: The inhibitory effect of IL-1β on A375-S2 cell growth was in a dose and time-dependent manner, and cell death rate reached more than 90% at 72 h after treatment with 10-9mol/L IL-1β. The inhibitors of caspase-family, -1, -3, -8, -9, and -10, partially blocked cell death at early stage. LDH assay showed that major IL-1β-induced cell death was apoptosis, and in a dose and time-dependent manner. Typical apoptotic DNA ladder was observed in agarose gel electrophoresis. CONCLUSION: IL-1β induced apoptosis in melanoma A375-S2 cells by activating caspase pathway. 相似文献
6.
T. Honda T. Nomura Y. Yamaguchi F. Mukai 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2004,121(4):242-252
The gene pool of the Japanese Black cattle has been completely closed to foreign breeds during the last 100 years. Genetic diversity of the Japanese Black cattle from 1960 to 2000 was monitored with three estimates of effective number of ancestors. Founder genome equivalent (Nge) accounts for all the causes of reduction of diversity. Effective number of founders (Nef) and non‐founders (Nenf) explain reduced diversity because of unequal genetic contributions of founders and random genetic drift in non‐founders, respectively. Further examination using gene dropping simulation was conducted to obtain information on survival of founder alleles. Unique founder alleles were dropped down along the actual pedigree with Monte Carlo procedure following Mendelian segregation rules, and generated genotypes of all the current live animals (612 959 heads). Pedigree records consisted of 2 075 188 animals was used for these analysis. The estimates of three effective numbers (Nef, Nge, and Nenf) decreased from 418.6 to 50.3, 86.6 to 7.3, and 109.2 to 8.5, respectively, during the period 1960–2000. The increasing differences between two kinds of genetic diversity indices derived from Nge and Nef showed that large part of the reduced diversity from 1980 was attributed to genetic drift caused by the intensive use of particular limited number of sires. In gene dropping analysis, probabilities of extinction of founder alleles were derived from their distributions of frequency in the current animals. Several founders showed low probabilities of allele extinction, irrespective of their relatively low genetic contributions. This suggests that these founders have lineages through which their alleles are surely transmitted to the current breed. The use of these founders as a strategy for recovering the genetic diversity was discussed. 相似文献
7.
Yosuke SASAKI Satoshi SEKIGUCHI Ryoko UEMURA Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):333-335
This study compared the reproductive and growth performances of pigs before and after depopulation and
restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after
depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and
restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and
respiratory syndrome and Aujeszky’s disease, remained undetected on the farms. Compared with before
depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born
was higher, the postweaning mortality rate was lower, and the age at slaughter was lower
(P<0.05). In summary, depopulation and restocking improved the reproductive and growth
performances of pigs. 相似文献
8.
9.
Chiaki Narabe Saki Kamiyama Mizuki Saito Phoompong Boonsaen Anchalee Khongpradit Suriya Sawanon Yutaka Suzuki Satoshi Koike Yasuo Kobayashi 《Animal Science Journal》2021,92(1):e13614
The methane-mitigating potency of cashew nutshell liquid (CNSL) was evaluated by investigating gas production from batch cultures using feces from Thai native ruminants that had been incubated for different periods. Feces was obtained from four Thai native cattle and four swamp buffaloes reared under practical feeding conditions at the Kasetsart University farm, Thailand. Fecal slurry from the same farm was also included in the analysis. CNSL addition successfully suppressed the methane production potential of feces from both ruminants by shifting short chain fatty acid profiles towards propionate production. Methane mitigation continued for almost 150 days, although the degree of mitigation was more apparent from Day 0 to Day 30. Bacterial and archaeal community shifts with CNSL addition were observed in feces from both ruminants; specifically, Bacteroides increased, whereas Lachnospiraceae and Ruminococcaceae decreased in feces to which CNSL was added. Fecal slurry did not show marked changes in gas production with CNSL addition. The findings showed that the addition of CNSL to the feces of ruminants native to the Southeast Asian region can suppress methane emission. Because CNSL can be easily obtained as a byproduct of the local cashew industry in this region, its on-site application might be ideal. 相似文献
10.
Makimi Wakai Shusuke Hayashi Yuko Chiba Satoshi Koike Kyo Nagashima Yasuo Kobayashi 《Animal Science Journal》2021,92(1):e13598
The growth and morphology of rumen methanogenic archaea (15 strains of 10 species in 5 genera, including 7 strains newly isolated in the present study) and bacteria (14 species in 12 genera) were investigated using unsupplemented in vitro pure cultures and cultures supplemented with cashew nut shell liquid (CNSL) and its phenolic compound components, anti-methanogenic agents for ruminant animals. Growth of most of the methanogens tested was inhibited by CNSL and alkylphenols at different concentrations ranging from 1.56 to 12.5 μg/ml. Of the alkylphenols tested, anacardic acid exhibited the most potent growth inhibition. Three gram-negative bacterial species involved in propionate production were resistant to CNSL and alkylphenols (>50 μg/ml). All the methanogens and bacteria that were sensitive to CNSL and alkylphenols exhibited altered morphology; disruption of the cell surface was notable, possibly due to surfactant activity of the tested materials. Cells division was inhibited in some organisms, with cell elongation and unclear septum formation observed. These results indicate that CNSL and alkylphenols, particularly anacardic acid, inhibit both rumen bacteria and methanogens in a selective manner, which could help mitigate rumen methane generation. 相似文献