Status,structure and environmental variations in semi-arid mangroves of India     

G.A.Thivakaran  Seema B.Sharma  Abhiroop Chowdhury  Arumugam Murugan 《林业研究》2020,31(1):163-173

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Reticuloendotheliosis virus integration in the fowl poxvirus genome: not a recent event   总被引：3，自引：0，他引：3  

Kim TJ  Tripathy DN 《Avian diseases》2001,45(3):663-669

Integration of reticuloendotheliosis virus (REV) into the genome of fowl poxvirus (FPV) has been reported recently. With a view to determine whether this event had occurred in the past, we screened by polymerase chain reaction (PCR) for the presence of REV provirus in the DNAs of nine avian poxviruses, some of which had been lyophilized 50 yr ago. For REV, 5' long terminal repeat (LTR) and REV envelope sequences were amplified, whereas for FPV, the major envelope antigen gene and the region flanking REV sequences were amplified. In six of seven FPV strains examined, the specific PCR amplicons were obtained for both REV provirus and FPV sequences. One isolate in which presence of REV 5' LTR and envelope was not detected by PCR, a LTR remnant was detected by Southern hybridization. Interestingly, no REV sequence was detected in either canary poxvirus or pigeon poxvirus genome. These observations indicate that REV integration in the FPV genome is not a recent phenomenon but probably occurred prior to 1949.  相似文献   

Studies of Leptospira illini, strain 3055: pathogenicity for different animals   总被引：1，自引：0，他引：1  

D N Tripathy  L E Hanson 《American journal of veterinary research》1973,34(4):557-562

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A consideration of previously uncharacterized fowl poxvirus unidirectional and bidirectional late promoters for inclusion in homologous recombinant vaccines     

Srinivasan V  Schnitzlein WM  Tripathy DN 《Avian diseases》2003,47(2):286-295

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Characterization of an avianpox virus isolated from an Andean condor (Vultur gryphus)     

Kim TJ  Schnitzlein WM  McAloose D  Pessier AP  Tripathy DN 《Veterinary microbiology》2003,96(3):237-246

A novel pox virus, condorpox virus (CPV) isolated from the spleen of an Andean condor (Vultur gryphus) by inoculation of chorioallantoic membranes (CAM) of specific pathogen free (SPF) chicken embryos was compared biologically, antigenically and genetically with fowlpox virus (FPV), the type species of the genus Avipoxvirus. Susceptible chickens inoculated with CPV developed only mild localized lesions but were not protected against subsequent challenge with FPV. Based on Western blotting, in addition to the presence of cross-reacting antigens, distinct differences in antigenic profiles of CPV and FPV were observed. Sequence analysis of a 4.5 kb HindIII fragment of CPV genomic DNA revealed the presence of eight co-linear genes corresponding to FPV open reading frame (ORF)193-198, 201 and 203. Interestingly, reticuloendotheliosis virus (REV) sequences present in the genome of all FPV were absent in CPV. Although, the results of a phylogenic analysis suggested that CPV is a member of the genus Avipoxvirus, its unique antigenic, biologic and genetic characteristics distinguish it from FPV to be considered as a new member of this genus.  相似文献   

Construction and immunogenicity studies of recombinant fowl poxvirus containing the S1 gene of Massachusetts 41 strain of infectious bronchitis virus   总被引：9，自引：0，他引：9  

Wang X  Schnitzlein WM  Tripathy DN  Girshick T  Khan MI 《Avian diseases》2002,46(4):831-838

The spike 1 (S1) surface glycoprotein of infectious bronchitis virus (IBV) is the major inducer of the generation of virus neutralizing antibodies, and the administration of purified S1 has been shown to elicit a protective immune response against virulent virus challenge. On the basis of these observations, recombinant fowl poxvirus (rFPV) containing a cDNA copy of the S1 gene of IBV Mass 41 (rFPV-S1) was constructed and its immunogenicity and vaccine potential were evaluated. Initially, rFPV-S1 was shown to express the S1 in vito by indirect immunofluorescence staining and western blot analyses. Later, in vivo expression was demonstrated by the detection of IBV-specific serum immunoglobulin G and neutralization antibodies in the sera of chickens immunized with rFPV-S1. That the recombinant virus elicited anti-IBV protective immunity was indicated by the manifested, relatively mild clinical signs of disease, decreased titers of recovered challenge virus, and less severe histologic changes of the tracheas in virulent IBV Mass 41-challenged chickens previously receiving rFPV-S1 as compared with parental fowl poxvirus (FPV)-vaccinated control birds. In contrast, chickens immunized with either recombinant or parental FPV were resistant to a subsequent virulent FPV challenge. As to a preferred method of immunization, wing web administration appeared to be superior to the subcutaneous route because a greater percentage of birds vaccinated by the former protocol exhibited an anti-IBV humoral immune response. Thus, rFPV-S1 has potential as a poultry vaccine against both fowl pox and infectious bronchitis.  相似文献   

Genetic and antigenic characterization of a poxvirus isolate from ostriches     

Shivaprasad HL  Kim TJ  Woolcock PR  Tripathy DN 《Avian diseases》2002,46(2):429-436

Avian poxvirus was isolated from nodules on the heads and conjunctiva of two 3-to-4-wk-old ostrich chicks. The ostriches from which poxvirus was isolated had been placed on premises where turkeys that had shown evidence of poxvirus infection had been raised earlier. Microscopically, the nodules from the ostriches were composed of proliferating and hypertrophic epithelial cells that formed large fronds. Most of the hypertrophic epithelial cells contained large eosinophilic intracytoplasmic inclusion bodies characteristic of poxvirus. Characterization of the avian poxvirus isolated from the cutaneous lesions in ostriches was based on western blotting of virus antigen, restriction fragment length polymorphism of genomic DNA, pathogenesis, and cross-protection studies in chickens. Antigenic and genetic studies did not reveal any significant difference between the poxvirus isolated from ostriches (PVO) and fowl poxvirus (FPV). Further, susceptible chickens immunized with the PVO were protected when challenged with a virulent strain of FPV. Thus, the poxvirus isolated from ostriches had similar antigenic, genetic, and biological properties to FPV.  相似文献   

Expression of avian influenza virus hemagglutinin by recombinant fowlpox virus   总被引：13，自引：0，他引：13  

D N Tripathy  W M Schnitzlein 《Avian diseases》1991,35(1):186-191

A vaccine strain of fowlpox virus (FPV) was genetically engineered to produce avian influenza virus hemagglutinin (HA). This was accomplished by inserting a cDNA copy of the avian influenza virus HA gene, which was regulated by a vaccinia virus promoter, into the FPV thymidine kinase (TK) gene. Two types of recombinant viruses, differing only in the orientation of the HA gene relative to an adjacent foreign gene (lacZ), were created. Following preliminary identification of FPV recombinants based on the generation of beta-galactosidase (lacZ gene product), correct insertion of the HA gene into the genomes of these viruses was verified by hybridization studies. Susceptible chickens vaccinated with these FPV recombinants produced specific hemagglutination-inhibiting antibodies against the HA antigen. In view of this immune response, these viruses may serve as vaccines against avian influenza virus. In this regard, they appeared to be less virulent than the parental virus.  相似文献   

Selection of diverse parents of chickpea (Cicer arietinum L.) by multivariate analysis and the degree of heterosis of their F1 hybrids     

Imtiaz Ahmed Khan  Seema Imtiaz  Bashir Ahmed Malik 《Euphytica》1990,51(3):227-233

Summary Studies on genetic diversity in chickpea (Cicer arietinum L.) indicated the existence of considerable amount of variation for grain yield and its components in the material. One hundred and thirty two genotypes fell into eight clusters. The covariation structure studied by means of factor analysis indicated the possibility of obtaining, through hybridization, genotypes physiologically and morphologically more efficient. Multivariate analysis of data from 7 parents and 21 F1 hybrids indicated weak correspondence between D²-analysis and canonical variate analysis. As there was no relationship between heterosis over midparent and genetic distance between the parents, so the traditional approach of making a large number of crosses is being suggested.  相似文献   

Effect of water and nitrogen management on aggregate size and carbon enrichment of soil in rice‐wheat cropping system     

Rojalin Tripathy  Anil Kumar Singh 《植物养料与土壤学杂志》2004,167(2):216-228

A study was carried out on a silty clay loam soil (Typic Haplustept) to evaluate the effect of farmyard manure (FYM) vis‐à‐vis fertilizer and irrigation application on the soil organic C content and soil structure. The fertilizer treatments comprised of eight different combinations of N and FYM and three water regimes. The results indicated that the application of FYM and increasing N rate increased soil organic carbon (SOC) content. Addition of FYM also increased the percentage of large sized water stable aggregates (> 5 mm) and reduced the percentage of smaller size aggregates. This was reflected in an increase in the mean weight diameter (MWD) and improved soil structure. The organic carbon content in macroaggregates (> 1 mm) was greater compared to microaggregates, and it declined with decrease in size of microaggregates. This difference in organic C content between macro‐ and microaggregates was more with higher N dose and FYM treated plots. The effect of residual FYM on MWD and organic C content of the soil after wheat harvest was not significant. The effect was less in deeper layers compared to surface layers of the soil. MWD was significantly correlated with the SOC content for the top two layers.  相似文献