The antioxidant system of sterlet seminal fluid in testes and Wolffian ducts     

Viktoriya Dzyuba  Borys Dzyuba  Jacky Cosson  Sergii Boryshpolets  Gunes Yamaner  Vitaliy Kholodniy  Marek Rodina 《Fish physiology and biochemistry》2014,40(6):1731-1739

Oxidative stress is a possible source of spermatozoa function deterioration. Seminal fluid (SF) protects spermatozoa against reactive oxygen species (ROS) attack during development in testes and transit through the reproductive tract. Spermatozoa curvilinear velocity and percent of motile cells as well as changes in thiobarbituric acid-reactive substance (TBARS) content, superoxide dismutase, and catalase activity, and uric acid concentration in SF were evaluated in sterlet sperm collected from testes 24 h after hormone induction of spermiation and from Wolffian ducts at 12, 24, 36, and 60 h after hormone injection (HI). While testicular spermatozoa motility was not initiated in activating medium, Wolffian duct sperm showed low motility at 12 h, significant increase at 24 and 36 h, and decrease at 60 h. Testicular SF was characterized by the highest level of TBARS and activity of studied enzymes compared with SF from Wolffian duct sperm at 24 h post-HI. In fluid from Wolffian duct sperm, a significant increase in TBARS content was shown at 36–60 h post-HI. In contrast to testicular SF, in SF from Wolffian duct sperm, this increase was not counterbalanced by changes in the studied variables of antioxidant system. This may be the source of the observed decrease in spermatozoa motility parameters 60 h post-HI. The results may confirm a dual role of ROS in fish sperm physiology. The data with respect to decrease in sturgeon spermatozoa motility parameters at 60 h post-HI should be taken into account in artificial sturgeon propagation.  相似文献   

Osmoregulation in fish sperm     

Herrera  Fabio  Bondarenko  Olga  Boryshpolets  Sergii 《Fish physiology and biochemistry》2021,47(3):785-795

Fish Physiology and Biochemistry - In most fish exhibiting external fertilization, spermatozoa become motile after release into water, triggered by differences between intracellular and...  相似文献   

Egg-sperm interaction in sturgeon: role of ovarian fluid     

Kholodnyy  Vitaliy  Dzyuba  Borys  Gadêlha  Hermes  Cosson  Jacky  Boryshpolets  Sergii 《Fish physiology and biochemistry》2021,47(3):653-669

Fish Physiology and Biochemistry - Fertilization of freshwater fish occurs in the environment which negatively affects a lifespan of gametes mostly due to the osmotic shock; therefore, male gametes...  相似文献