Effect of temperature on the incubation period and leaf colonization in bacterial blight of anthurium     

Fukui R  Fukui H  Alvarez AM 《Phytopathology》1999,89(11):1007-1014

ABSTRACT Effect of temperature on leaf colonization in anthurium blight was studied using a bioluminescent strain of Xanthomonas campestris pv. dieffenbachiae. In a susceptible cultivar, colonization of leaf tissues (monitored by detection of bioluminescence) and symptom development (assessed visually) advanced rapidly at higher temperatures. For a susceptible cultivar, there was a linear relationship between degree-days and percent leaf area colonized by the pathogen, indicating that leaf colonization in a susceptible cultivar was a direct function of the cumulative effect of temperature. The degree-day intercept of the regression line represented the time from inoculation to detection of bioluminescence, and the slope indicated the increase of leaf colonization per degree-day. There also was a linear relationship between the logarithm of degree-days and the logarithm of percent leaf area showing visible symptoms in a susceptible cultivar. The degree-day intercept of this relationship represented the incubation period (about 500 degree-days). The degree-days required to detect bioluminescence was not considerably different between susceptible and resistant cultivars. However, the subsequent rates of leaf colonization were significantly lower for a resistant cultivar than for a susceptible cultivar in all temperature regimes. The results suggest that multiplication of the pathogen in the leaf tissues is optimized in the susceptible cultivar. In contrast, in the resistant cultivar, the defense mechanisms overshadow the temperature effect. The differential response to temperatures may be an additional indicator of cultivar susceptibility.  相似文献   

Effect of activation methods for bovine oocytes after intracytoplasmic injection   总被引：2，自引：0，他引：2  

Ikumi S  Asada M  Sawai K  Fukui Y 《The Journal of reproduction and development》2003,49(1):37-43

A principal nuclear transfer procedure is to inject a donor cell into the perivitelline space in an enucleated oocyte and then electric fusion is performed (cell fusion method). The effects of activation methods in reconstructed oocytes for the serum-starved somatic cell cloning procedure were investigated in this study by means of intracytoplasmic injection (i.c.i.). Bovine oocytes were enucleated at 18-22 h for in vitro maturation, and subsequently the nucleus of cumulus cell collected from Japanese Black Bulls (JBCC) after 5-7 days of starved culture was injected into the recipient cytoplast with a piezo-micromanipulator. At 1 h after i.c.i., reconstructed oocytes were stimulated with ethanol (ET) or calcium ionophore (CaI) as the first activation treatment, followed by cycloheximide (CHX) or 6-dimethylaminopurin (DMAP) treatment as the second activation. In the experiment on the first activation method, the proportion of reconstructed oocytes developing to the blastocyst stage was significantly (p<0.01) higher in the ET activation method than that with CaI (10.5% and 4.7%, respectively). And the experiment on the second activation method after ET treatment showed similar proportions of blastocyst development in both CHX and DMAP treatments (5.9% and 2.8%, respectively). The present results indicated that combined activation treatment with ET and CHX was efficient for reconstructed bovine oocytes by i.c.i.  相似文献   

The pharmacological effects of the anesthetic alfaxalone after intramuscular administration to dogs     

Jun TAMURA  Tomohito ISHIZUKA  Sho FUKUI  Norihiko OYAMA  Kodai KAWASE  Kenjiro MIYOSHI  Tadashi SANO  Kirby PASLOSKE  Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):289-296

The pharmacological effects of the anesthetic alfaxalone were evaluated after intramuscular (IM) administration to 6 healthy beagle dogs. The dogs received three IM doses each of alfaxalone at increasing dose rates of 5 mg/kg (IM5), 7.5 mg/kg (IM7.5) and 10 mg/kg (IM10) every other day. Anesthetic effect was subjectively evaluated by using an ordinal scoring system to determine the degree of neuro-depression and the quality of anesthetic induction and recovery from anesthesia. Cardiorespiratory variables were measured using noninvasive methods. Alfaxalone administered IM produced dose-dependent neuro-depression and lateral recumbency (i.e., 36 ± 28 min, 87 ± 26 min and 115 ± 29 min after the IM5, IM7.5 and IM10 treatments, respectively). The endotracheal tube was tolerated in all dogs for 46 ± 20 and 58 ± 21 min after the IM7.5 and IM10 treatments, respectively. It was not possible to place endotracheal tubes in 5 of the 6 dogs after the IM5 treatment. Most cardiorespiratory variables remained within clinically acceptable ranges, but hypoxemia was observed by pulse oximetry for 5 to 10 min in 2 dogs receiving the IM10 treatment. Dose-dependent decreases in rectal temperature, respiratory rate and arterial blood pressure also occurred. The quality of recovery was considered satisfactory in all dogs receiving each treatment; all the dog exhibited transient muscular tremors and staggering gait. In conclusion, IM alfaxalone produced a dose-dependent anesthetic effect with relatively mild cardiorespiratory depression in dogs. However, hypoxemia may occur at higher IM doses of alfaxalone.  相似文献   

Central venous blood gas and acid-base status in conscious dogs and cats     

Jun TAMURA  Takaharu ITAMI  Tomohito ISHIZUKA  Sho FUKUI  Kenjirou MIYOSHI  Tadashi SANO  Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):865-869

To determine the reference level of central venous oxygen saturation (ScvO₂) and clinical efficacy of central venous blood gas analysis, partial pressures of oxygen and carbon dioxide, pH, oxygen saturation, base excess (B.E.) and HCO₃ concentration were compared between simultaneously obtained central venous and arterial blood samples from conscious healthy 6 dogs and 5 cats. Comparisons between arteriovenous samples were performed by a paired t-test and Bland-Altman analysis. Between arteriovenous samples, B.E. showed good agreement, but there were significant differences in other parameters in the dogs, and no good agreement was detected in cats. The ScvO₂ in dogs and cats were 82.3 ± 3.5 and 62.4 ± 13.5%, respectively. Central venous blood gas analysis is indispensable, especially in cats.  相似文献   

Sparing Effect of Robenacoxib on the Minimum Alveolar Concentration for Blunting Adrenergic Response (MAC-BAR) of Sevoflurane in Dogs     

Jun TAMURA  Takaharu ITAMI  Tomohito ISHIZUKA  Sho FUKUI  Norihiko OOYAMA  Kenjiro MIYOSHI  Tadashi SANO  Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):113-117

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Production and characterization of alien chromosome addition lines in Allium fistulosum carrying extra chromosomes of Allium roylei using molecular and cytogenetic analyses     

Nur Aeni Ariyanti  Vu Quynh Hoa  Ludmila I. Khrustaleva  Sho Hirata  Mostafa Abdelrahman  Shin-ichi Ito  Naoki Yamauchi  Masayoshi Shigyo 《Euphytica》2015,206(2):343-355

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Tracks of cosmic rays in plastics     

Fleischer RL  Price PB  Walker RM  Filz RC  Fukui K  Friedlander MW  Holeman E  Rajan RS  Tamhane AS 《Science (New York, N.Y.)》1967,155(3759):187-189

Cosmic ray nuclei have been observed with the use of plastic trackdetecting solids in satellites and high-altitude balloon flights. Nuclear emulsions in the stacks of plastic sheets allowed the positive identification of cosmic raynuclei as light as nitrogen. The most striking new information was the failure to observe relativistic iron nuclei, a result which has led to an advance in the understanding of track registration criteria.  相似文献   

Aureusidin synthase: a polyphenol oxidase homolog responsible for flower coloration   总被引：1，自引：0，他引：1  

Nakayama T  Yonekura-Sakakibara K  Sato T  Kikuchi S  Fukui Y  Fukuchi-Mizutani M  Ueda T  Nakao M  Tanaka Y  Kusumi T  Nishino T 《Science (New York, N.Y.)》2000,290(5494):1163-1166

Aurones are plant flavonoids that provide yellow color to the flowers of some popular ornamental plants, such as snapdragon and cosmos. In this study, we have identified an enzyme responsible for the synthesis of aurone from chalcones in the yellow snapdragon flower. The enzyme (aureusidin synthase) is a 39-kilodalton, copper-containing glycoprotein catalyzing the hydroxylation and/or oxidative cyclization of the precursor chalcones, 2',4',6',4-tetrahydroxychalcone and 2',4',6',3,4-pentahydroxychalcone. The complementary DNA encoding aureusidin synthase is expressed in the petals of aurone-containing varieties. DNA sequence analysis revealed that aureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenol oxidase homolog in plants, i.e., flower coloration.  相似文献   

Discovery of a Jupiter/Saturn analog with gravitational microlensing     

Gaudi BS  Bennett DP  Udalski A  Gould A  Christie GW  Maoz D  Dong S  McCormick J  Szymanski MK  Tristram PJ  Nikolaev S  Paczynski B  Kubiak M  Pietrzynski G  Soszynski I  Szewczyk O  Ulaczyk K  Wyrzykowski L;OGLE Collaboration  Depoy DL  Han C  Kaspi S  Lee CU  Mallia F  Natusch T  Pogge RW  Park BG;MuFUN Collaboration  Abe F  Bond IA  Botzler CS  Fukui A  Hearnshaw JB  Itow Y  Kamiya K  Korpela AV  Kilmartin PM  Lin W  Masuda K  Matsubara Y  Motomura M  Muraki Y  Nakamura S  Okumura T  Ohnishi K  Rattenbury NJ  Sako T  Saito T  Sato S 《Science (New York, N.Y.)》2008,319(5865):927-930

Searches for extrasolar planets have uncovered an astonishing diversity of planetary systems, yet the frequency of solar system analogs remains unknown. The gravitational microlensing planet search method is potentially sensitive to multiple-planet systems containing analogs of all the solar system planets except Mercury. We report the detection of a multiple-planet system with microlensing. We identify two planets with masses of approximately 0.71 and approximately 0.27 times the mass of Jupiter and orbital separations of approximately 2.3 and approximately 4.6 astronomical units orbiting a primary star of mass approximately 0.50 solar mass at a distance of approximately 1.5 kiloparsecs. This system resembles a scaled version of our solar system in that the mass ratio, separation ratio, and equilibrium temperatures of the planets are similar to those of Jupiter and Saturn. These planets could not have been detected with other techniques; their discovery from only six confirmed microlensing planet detections suggests that solar system analogs may be common.  相似文献   

Inhibitory effects of oolong tea polyphenols on pancreatic lipase in vitro   总被引：10，自引：0，他引：10  

Nakai M  Fukui Y  Asami S  Toyoda-Ono Y  Iwashita T  Shibata H  Mitsunaga T  Hashimoto F  Kiso Y 《Journal of agricultural and food chemistry》2005,53(11):4593-4598

Fifty-four polyphenols isolated from tea leaves were evaluated for their inhibitory activities against pancreatic lipase, the key enzyme of lipid absorption in the gut. (-)-Epigallocatechin 3-O-gallate (EGCG), which is one of major polyphenols in green tea, showed lipase inhibition with an IC50 of 0.349 microM. Moreover, flavan-3-ol digallate esters, such as (-)-epigallocatechin-3,5-digallate, showed higher activities of inhibition on lipase with an IC50 of 0.098 microM. On the other hand, nonesterified flavan-3-ols, such as (+)-catechin, (-)-epicatechin, (+)-gallocatechin, and (-)-epigallocatechin, showed zero and/or the lowest activities against pancreatic lipase (IC50 > 20 microM). These data suggested that the presence of galloyl moieties within the structure was required for enhancement of pancreatic lipase inhibition. It is well-known that flavan-3-ols are polymerized by polyphenol oxidase and/or heating in a manufacturing process of oolong tea. Oolonghomobisflavans A and B and oolongtheanin 3'-O-gallate, which are typical in oolong tea leaves, showed strong inhibitory activities with IC50 values of 0.048, 0.108, and 0.068 microM, respectively, even higher than that of EGCG. The oolong tea polymerized polyphenols (OTPP) were prepared for the assay from oolong tea extract, from which the preparation effectively subtracted the zero and/or less-active monomeric flavan-3-ols by preparative high-performance liquid chromatography. The weight-average molecular weight (Mw) and number-average molecular-weight (Mn) values of OTPP were 2017 and 903, respectively, by using gel permeation choromatography. OTPP showed a 5-fold stronger inhibition against pancreatic lipase (IC50 = 0.28 microg/mL) by comparison with that of the tannase-treated OTPP (IC50 = 1.38 microg/mL). These data suggested that the presence of galloyl moieties within their chemical structures and/or the polymerization of flavan-3-ols were required for enhancement of pancreatic lipase inhibition.  相似文献