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1.
Scorza Júnior RP Jarvis NJ Boesten JJ van der Zee SE Roulier S 《Pest management science》2007,63(10):1011-1025
Testing of pesticide leaching models against comprehensive field-scale measurements is necessary to increase confidence in their predictive ability when used as regulatory tools. Version 5.1 of the MACRO model was tested against measurements of water flow and the behaviour of bromide, bentazone [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide] and imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] in a cracked clay soil. In keeping with EU (FOCUS) procedures, the model was first calibrated against the measured moisture profiles and bromide concentrations in soil and in drain water. Uncalibrated pesticide simulations based on laboratory measurements of sorption and degradation were then compared with field data on the leaching of bentazone and imidacloprid. Calibrated parameter values indicated that a high degree of physical non-equilibrium (i.e. strong macropore flow) was necessary to describe solute transport in this soil. Comparison of measured and simulated bentazone concentration profiles revealed that the bulk of the bentazone movement in this soil was underestimated by MACRO. Nevertheless, the model simulated the dynamics of the bentazone breakthrough in drain water rather well and, in particular, accurately simulated the timing and the concentration level of the early bentazone breakthrough in drain water. The imidacloprid concentration profiles and its persistence in soil were simulated well. Moreover, the timing of the early imidacloprid breakthrough in the drain water was simulated well, although the simulated concentrations were about 2-3 times larger than measured. Deep groundwater concentrations for all substances were underestimated by MACRO, although it simulated concentrations in the shallow groundwater reasonably well. It is concluded that, in the context of ecotoxicological risk assessments for surface water, MACRO can give reasonably good simulations of pesticide concentrations in water draining from cracking clay soils, but that prior calibration against hydrologic and tracer data is desirable to reduce uncertainty and improve accuracy. 相似文献
2.
Th.J.M. Schoenmakers G. Flik E.W. Roubos S.E. Wendelaar Bonga 《Fish physiology and biochemistry》1997,17(1-6):347-355
The role of calcium influx through voltage-dependent calcium ion channels in the exocytotic response of single isolated pars intermedia cells from the teleost tilapia (Oreochromis mossambicus) was investigated by means of the whole-cell patch-clamp technique and high resolution electrical measurements. Calcium currents differed from barium currents in several ways: the peak ICa was smaller, the current-voltage relationship for calcium attained its maximum at +10 mV instead of 0 mV, and the inward calcium current inactivated more rapidly. Electron micrographs showed that pars intermedia cells possess dense, encored vesicles with an average diameter of 140 nm. Influx of calcium resulted in an increased cell membrane capacitance (Cm) after the depolarizing period, indicating a fast exocytotic response. Comparison with late recordings revealed the presence of a transient in Cm, presumably attributable to movement of ion channel gates. The average increase in Cm was 13.4 fF, suggesting the fusion of at least 23 vesicles with the plasma membrane during the depolarizing pulse. In contrast to calcium, barium did not support significant exocytosis. We conclude that calcium entry through voltage-dependent calcium channels rapidly leads to the exocytosis of secretory vesicles from tilapia pars intermedia cells. 相似文献
3.
P. H. M. Balm P. Pepels E. van Lieshout S. E. Wendelaar Bonga 《Fish physiology and biochemistry》1993,11(1-6):125-130
This study describes the effects of IL-1 (interleukin 1) and LPS (bacterial endotoxin lipopolysaccharide) on the release of
α-MSH (alpha melanocyte stimulating hormone) from the neurointermediate lobe (NIL) of the teleost Oreochromis mossambicus (tilapia). In vivo treatment of tilapia with IL-1 for 8 days led to a 49% inhibition of basal α-MSH release, measured by means of an in vitro micro-superfusion technique. The treatment did not affect the sensitivity of the tissue to TRH. In vitro, the release of α-MSH was inhibited by LPS in a dose dependent manner. In addition to its effects on the unstimulated release
of the hormone, LPS also blunted the response to a TRH stimulation. Together with recent results obtained by others demonstrating
the effects of (neuro-)peptides on immune parameters and the presence of cytokines in fish, the present data establish the
bidirectional character of the communication between the immune and the (neuro-)endocrine systems in teleosts.
Résumé Cette étude décrit les effets de l'IL-1 (interleukin 1) et du LPS (bacterial endotoxin lipopolysaccharide) sur la libération de α-MSH (alpha melanocyte stimulating hormone) par le lobe neurointermédiaire (NIL) d'un téléosteen Oreochromis mossambicus (tilapia). Le traitement in vivo du tilapia avec l'IL-1 pendent 8 jours conduit à une inhibition de 49% de la libération basale d'α-MSH mesurée à l'aide d'une technique in vitro de micro-superfusion. Ce traitement ne modifie pas la sensibilité du tissue au TRH. In vitro, la libération de α-MSH est inhibée par le LPS de manière dose-dépendante. En plus de ses effets sur la libération basale de α-MSH, le LPS bloque aussi la réponse à une stimulation par le TRH. Confrontés à des donnés récemment publiés montrant les effets de (neuro-)peptides sur les paramêtres immunitaires et la présence de cytokines chez les poissons, nos résultats établissent le caractère bidirectionnel de la communication entre le système immunitaire et les systèmes (neuro-)endocriniens.相似文献
4.
X.J.H.X. Stouthart M.A.J. Huijbregts P.H.M. Balm R.A.C. Lock S.E. Wendelaar Bonga 《Fish physiology and biochemistry》1998,18(4):321-329
To investigate whether PCB 126 exposure duringembryonic development induces an endocrine stressresponse in larval carp, eggs were exposed,containing 0.01% ethanol (vehicle-control), 10-11,immediately after fertilization, for 48 h to water10-10 or 10-9 mol l-1 PCB in 0.01% ethanol. Eggsincubated in water served as controls. After transferto PCB-free water, mortality, the incidence ofyolk-sac and pe-ricardial oedema, wet and dry weight,rate of skin pigmentation, and whole-body contents ofthe stress hormones ACTH, -MSH and cortisol weredetermined at 48, 96, 144, 168, 192 and 216 hpost-fertilization. Except for the dry weight, allparameters of animals exposed to 10-10 and 10-9 moll-1 PCB increased in a concentration-related manner.However, these changes became evident only at 144 hpost-fertilization, i.e. after resorption of theyolk-sac. Swelling of the yolk sac and pericardiumoccurred, and whole-body ACTH, -MSH and cortisollevels increased. Although animals exposed to 10-10and 10-9 mol l-1 PCB displayed stable but elevatedwhole-body ACTH and -MSH levels until 216 h,whole-body cortisol concentrations gradually decreasedfrom 168 h post-fertilization, and were significantlybelow control values at 216 h post-fertilization.Exposure of the carp embryos to 10-11 mol l-1 PCB only increased whole-body -MSH levels. Increased whole-body ACTH and cortisol levels indicate that PCBinduces a stress response in carp larvae, possiblymediated by a disturbed hydromineral balance (oedema).We further suggest that the PCB-stimulated bodypigmentation is mediated by a stimulation of -MSHsecretion. 相似文献
5.
V. Szisch A.L. van der Salm S.E. Wendelaar Bonga M. Pavlidis 《Fish physiology and biochemistry》2002,27(1-2):1-8
The effect of lighting spectrum (full vs. blue) on skin colour and stress response in red porgy, Pagrus pagrus, held under a 12L:12D photoperiod was investigated. Fish exposed to blue lighting spectrum became gradually paler with a maximum lightness value (L = 45.33) on day 27, significantly higher than control fish (L = 31.49). However, there was no difference in skin melanin content, hue and chroma among the experimental groups. There was also no statistically significant effect of lighting spectrum on average glucose, thyroxine (T4), triiodothyronine (T3), cortisol (F) and Melanophore Stimulating Hormone (MSH) plasma concentrations nor in the pattern of changes during the time course of the experiment. Results showed that lighting spectrum affects skin lightness but not the chromaticity attributes hue and chroma. This effect is due to changes in the motility of the melanophores (the area occupied by melanosomes) and is not mediated by MSH or the general stress response. 相似文献
6.
P. M. Verbost M. H. G. Berntssen F. Kroglund E. Lydersen H. E. Witters B. O. Rosseland B. Salbu S. E. Wendelaar Bonga 《Water, air, and soil pollution》1995,85(2):341-346
Mixing of acid river water containing aluminium (pH 5.1, Al 345 g.l–1) with neutral water of a lake (pH 7.0, Al 73 g.l–1) resulted in water (pH 6.4, Al 245 g.l–1) with a pH (6.4) and Al concentration (245 g.l–1) expected to have low toxicity to fish on the basis of current Al toxicity models. However, under semi-field conditions the freshly mixed water (a few sec. after mixing) proved to be highly toxic to brown trout. The fish were exposed to the water at different places along a 30 m channel. At the beginning of the channel acid and neutral water were continuously mixed; the mixed water left the channel after 340 sec. The cells of the gills showed a highly increased rate of cell death by apoptosis and necrosis. Intercellular spaces were enlarged, and many leucocytes penetrated in these spaces. Mucus release was stimulated to depletion. Plasma chloride levels were hardly affected. There was a clear gradient in the deleterious effects on the fish along the channel. The fish at the beginning of the channel (about 12 sec. after mixing of the water), were severely affected, whereas the fish kept at the end of the channel (340 sec. after mixing) were only mildly affected. In the natural situation fish will relatively quickly pass through a mixing zone. In our study we therefore focused on the effects on fish after a 60 min exposure to a mixing zone (5 sec after mixing), with subsequent recovery in a region downstream of the confluence and in neutral water with low Al. The recovery in the downstream area (at the end of the channel, i.e. 5 min after mixing) was clearly hampered when compared to the recovery in neutral water with low aluminium. Thus, a short exposure to the toxic mixing zone followed by a stay in water downstream of this zone, as may occur in nature, is detrimental to migrating trout. We conclude that freshly mixed acid and neutral water contain toxic components during the first seconds to minutes after mixing, that can not be explained by current models on aluminium toxicity. 相似文献
7.
Van Anholt RD Spanings FA Nixon O Wendelaar Bonga SE Koven WM 《Fish physiology and biochemistry》2012,38(3):703-713
In previous studies in freshwater tilapia (Oreochromis mossambicus), dietary supplementation with arachidonic acid (ArA; 20:4n?-?6) had considerable, opposing effects on the main ion-transporting enzyme Na(+)/K(+)-ATPase in gills and kidneys and changed the release of osmoregulatory hormones, such as cortisol. The present study was performed to assess the influence of dietary ArA on (1) the osmoregulatory capacity of tilapia acclimated to seawater (SW) (34‰) and (2) the osmoregulatory imbalance associated with acute stress. The increased ambient salinity was associated with significant alterations in the tissue fatty acid composition, particularly the n?-?6 polyunsaturated fatty acids (PUFAs). Tissue levels of ArA were further increased as a result of dietary supplementation, whereas docosahexaenoic acid (DHA, 22:6n?-?3) and eicosapentaenoic acid (EPA, 20:5n?-?3) decreased in gills and kidneys. Basal plasma cortisol as well as lactate levels were elevated in the ArA-supplemented SW-acclimated tilapia compared with the control group. The 5?min of confinement (transient stress) increased plasma cortisol, glucose, and lactate levels with significantly higher levels in ArA-supplemented tilapia. Confinement was also associated with significantly elevated plasma osmolality, sodium, chloride, and potassium levels. ArA-supplemented tilapia showed markedly lower ionic disturbances after confinement, suggesting that dietary ArA can attenuate the hydromineral imbalance associated with acute stress. These results emphasize the involvement of ArA and/or its metabolites in the endocrine and osmoregulatory processes and the response to confinement stress. 相似文献
8.
A L Van Der Salm D T Nolan F A T Spanings S E Wendelaar Bonga 《Journal of fish diseases》2000,23(3):173-184
The host‐parasite interaction between juvenile carp, Cyprinus carpio, and the ectoparasitic branchiuran, Argulus japonicus, together with the role of cortisol in this interaction, was examined at the level of the host skin epidermis. Epidermal mucous cell numbers, and proliferation and apoptosis of the epithelial cells were studied over 32 days. Apoptotic cell numbers in the uppermost epidermis were reduced at 26 days post‐infection with A. japonicus, while the other parameters were unaffected. Administration of cortisol‐containing food resulted in reduced apoptosis in the cells in the upper skin epidermis at 24 h and at 28 days post‐feeding. Cortisol feeding combined with A. japonicus infection reduced numbers of apoptotic cells in the upper epidermis more than either individual treatment. Further, combining the treatments also significantly increased apoptosis in the lower epidermis in cells morphologically identified as leucocytes apparently migrating macrophages and lymphocytes. Using immunohistochemistry, we demonstrated cortisol receptor presence and cellular localization in the teleost epidermis. Receptors only occurred in pavement cells in the upper epidermis and in leucocytes in the lower parts of the epidermis. The ectoparasites, or administered cortisol, induced effects which may be functionally adaptive in the upper pavement cells, while combining the two treatments also induced changes indicative of immunosuppression. 相似文献
9.
Dr. Paul Balm Nico Goossen Sjef van de Rijke Sjoerd Wendelaar Bonga 《Fish physiology and biochemistry》1988,5(1):31-38
Branchial plasma membranes from the freshwater cichlid teleostOreochromis mossambicus (tilapia) contain two Na+-dependent ATPases: Na+/K+ ATPase, and an amiloride-sensitive ATPase which is postulated to operate as a Na+/H+ (–NH4
+) ATPase. It is suggested that both enzyme activities are located in the basolateral membrane system of the chloride cells. K+ has opposing effects on the two enzymes: it stimulates Na+/K+ ATPase and inhibits Na+/H+ (–NH4
+) ATPase activity. Na+/H+ ATPase appears more sensitive to NH4
+ at low concentrations than Na+/K+ ATPase and the stimulatory effect by NH4
+ ions on the first enzyme could be important in facilitating NH4
+ excretion by tilapia gills under physiological conditions.In vitro maximum stimulation by NH4
+ is similar for the two enzymes (200%). In contrast to Na+/K+ ATPase, Na+/H+ ATPase activity is inhibited by supra-physiological (>20 mM) concentrations of NH4
+. 相似文献
10.
R. G. J. M. Hanssen N. Mayer-Gostan G. Flik S. E. Wendelaar Bonga 《Fish physiology and biochemistry》1993,10(6):491-496
The kinetics and hypocalcemic potency of stanniocalcin (STC) were examined in freshwater and seawater eels. The secretion rate and the metabolic clearance rate of STC were calculated from the STC disappearance curve after intra-arterial injection of trout STC. Basal plasma STC concentrations in freshwater and seawater eels did not differ but the STC secretion rate and metabolic clearance rate in seawater eel were 70–75% higher than in FW eel. The increased STC distribution space in seawater eels suggests that the STC receptor density was increased. STC had a higher hypocalcemic potency in seawater than in freshwater eels. These observations support the hypothesis that seawater fish require more hormonal control over transcellular influx of calcium than freshwater fish.To whom correspondence should be addressed. 相似文献