圈养华南虎血液生化指标正常参考值初步研究     

任小冬  黄勉  李石洲  雷胜桥  虞坚颐  刘力军 《林业与环境科学》2021,(1)

以国内部分圈养的健康华南虎Panthera tigris amoyensis作为研究对象,通过收集华南虎血液生化报告和采集华南虎血液样本检测建立样本数据,血样检测采用自动血液生化分析仪干式试剂片生化技术测定,测定血液生化指标包括血糖(GLU)、总蛋白(TP)、总胆固醇(CHOL)、总胆红素(TBIL)、肌酐(CREA)等。得到华南虎血液生化共38项指标的正常参考值范围(95%概率),其中几项主要指标正常值范围如下:GLU(2.82-11.55)mmol/L、TP(56.58-85.93)g/L、CHOL(3.32-8.66)μmol/L、TBIL(0-5.30)μmol/L、CREA(52-349)μmol/L等。统计结果显示,雌雄华南虎间血液生化指标的部分项目有差异(P <0.05),幼龄组和成年组间各项生化指标差异不显著(P >0.05),与其他虎亚种相关血液生化报道的生化指标部分有差别。华南虎血液生化指标正常参考值范围不同于其他虎亚种,建立华南虎血液生化指标正常值对华南虎的饲养管理、疾病诊治预防和保育繁殖研究工作有重要作用。  相似文献   

NaCl对不同抗性水稻氮代谢及相关基因表达的影响     

杨德翠  徐青  赵方贵  柳冕  刘新 《华北农学报》2020,35(2):187-195

为了探究盐胁迫下不同抗性水稻氮代谢的变化,以抗性品种东稻4号、盐敏感品种日本晴为材料,测定NaCl胁迫下水稻根和叶中氮含量、氮代谢相关酶活及相关基因表达的变化。结果表明,0.3%的NaCl胁迫后水稻根和叶中NO~-_3含量、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、依赖NADH的谷氨酸合酶(NADH-GOGAT)活性均有不同程度的下降,而NH~+_4含量、依赖Fd的谷氨酸合酶(Fd-GOGAT)、谷氨酸脱氢酶(GDH)、天冬氨酸转氨酶(AST)活性均升高,东稻4号氮含量及氮代谢酶活性比日本晴稳定,根和叶中变化一致。检测氮转运及代谢相关基因表达发现,硝酸根转运蛋白基因OsNRT1;1、OsNRT1;5、OsNRT1;8、OsNRT2;1,铵转运蛋白基因OsAMT1;1、OsAMT1;2及谷氨酰胺合成酶基因OsGS1;2、天冬氨酸转氨酶基因OsAS盐胁迫后在2个品种中均升高,在高表达部位升高显著;谷氨酸合酶基因OsFd-GOGAT在两品种叶中升高显著,根中稍有下降;谷氨酸合酶基因OsNADH-GOGAT和谷氨酸脱氢酶基因OsGDH在日本晴根中升高显著,在叶中及东稻4号中升高但不显著。OsNR1在NaCl胁迫后两品种叶中表达量显著降低,根中无差异。东稻4号OsNRTs及氮代谢相关基因表达量总体上高于日本晴,而日本晴OsAMTs升高幅度大于东稻4号。研究表明,NaCl胁迫下,东稻4号相比日本晴更能保持氮代谢稳定性,这可能是水稻抗性品种耐盐的一个重要机制。  相似文献   

Weeds of East Pakistan     

A. L. Mian 《国际虫害防治杂志》2013,59(4):486-489

Abstract

In a weed survey, 88 species of weeds, belonging to 30 families, were found growing in the arable lands of the East Pakistan Agricultural University Farms. In East Pakistan, hand-weeding is still almost the only means used for controlling weeds in crop fields. The results of trials conducted at the East Pakistan Agricultural University for controlling weeds in jute are reported.  相似文献   

辽东半岛结缕草无性系种群构件生物量相关模型及形成规律   总被引：5，自引：2，他引：5  

张学勇  杨允菲  张殿顺  张绵 《草原与草坪》2006,(3):23-26

按生物构件的理论,分析了辽东半岛结缕草无性系种群叶片、叶鞘、分株、匍匐茎各构件生物量以及总生物量间的相关模型和构件生物量的分配、形成规律,探讨了其不同生物量基于种群大小(密度)的形成规律。结果表明,辽东半岛结缕草无性系种群的总生物量及各构件生物量均随着种群密度的增大而以线性函数增加,各构件生物量与总生物量以及各构件生物量间亦均以线性函数增加,生物量增加比率均表现为:分株>匍匐茎,叶鞘>叶片,总生物量增量的27.2%分配给叶片,29.2%分配给叶鞘,43.6%分配给匍匐茎,叶片,叶鞘和匍匐茎构件生物量的积累是按1∶1.1∶1.6比例形成。  相似文献   

金钱豹血液理化值的测定   总被引：2，自引：1，他引：2  

王兴金  李少基  李婉萍  谢高基  陈武  黄勉  陈足金  陈绚姣 《野生动物》2006,27(2):33-35

广州动物园科研中心于2003年10月至2005年3月间共采集11只金钱豹的34份血液样本，对40项临床常用的血液生理生化值进行了测定，结果用SPSS11．0统计软件进行统计分析。  相似文献   

Sediment flow behavior in agro-watersheds of the purple soil region in China under different storm types and spatial scales     

Zhan-Bin Li  Peng Li  Jian-Gang Han  Mian Li   《Soil & Tillage Research》2009,105(2):285-291

Conservation tillage has proved to be an effective option to reduce erosion and particulate phosphorus (PP) losses from fields to watercourses. However, it usually leads to the higher losses of P in dissolved reactive (DRP) form if P accumulates into the uppermost soil layer.In this study we investigated the influence of CaCO₃-rich boardmill sludge (BMS, applied 7 years ago) on soil properties and percolate quality with soil samples as well as undisturbed soil columns taken from a long-term conservation-tilled clay field experiment. A short-term laboratory experiment was undertaken to evaluate whether newly added BMS (FS) can be used to improve the quality of percolate waters by reducing turbidity, PP and DRP.The hypothesis was that BMS reduces the risk of dissolved reactive phosphorus (DRP) leaching from conservation-tilled soil. BMS served as a liming agent and counteracted the no-till-induced acidification and accumulation of DRP in the surface soil layer. Surface soil pH was on average 0.3 units higher and soil Ca 500 mg kg⁻¹ higher in the BMS-treated plots than in the plots without BMS. The ability of BMS to enhance the availability of P for plants may prevent the surface accumulation of P in NT cultivation. At the same time, BMS-induced increase in Ca and EC of the surface soil suppress the diffuse double layer by reducing P desorption and leaching risk.In the laboratory experiment, FS reduced percolate turbidity by 80% and PP and DRP concentrations by 62% and 50%, respectively. FS probably improved stability of soil aggregates in the laboratory experiment, obviously through enhanced microbial activity. The covering effect and infiltration of water through the 0–5 cm soil layer seemed to be crucial factors in reducing P losses.BMS proved to be a promising material for the applications to the conservation-tilled fields. The effect of BMS on microbes and immobilization of nutrients, should, however, be studied further in fields under a wider diversity of conditions.  相似文献   

基于语义分割与实例分割的玉米茎秆截面参数测量方法     

陈燕  李想  曹勉  胡小春  王令强 《农业机械学报》2023,54(6):214-222

茎秆微观结构与其力学性能密切相关,影响作物的抗倒伏性能。但作物茎秆微观表型参数难以通过人工方式获取,因此急需自动化的测量方法。本研究以玉米为材料,通过光学显微镜获得玉米茎秆横截面切片图像,基于深度学习架构融合ResNet和Unet构建语义分割Res-Unet网络模型,对截面表皮、周皮和髓区3个功能区域进行分割;针对维管束数量多、面积小、密度大的特点,以EfficientDet作为基础网络架构,根据维管束尺寸小的特性,减少双向特征图金字塔(BiFPN)的层数,达到提高推理速度、减少显存占用量的目的,同时添加掩膜分割分支,构造新的网络Eiff-BiFPN实现对维管束的分割。实验结果表明,功能区域分割的平均DICE达到88.17%;维管束分割的AP₅₀和AP_50:70分别达到88.78%和72.80%。根据分割结果,可以获得玉米茎秆截面尺寸、各功能区域尺寸和维管束数量、面积等微观结构参数。本文方法具有精确性、实时性和可用性,可用于玉米茎秆微观结构参数的自动化测定,为作物抗倒伏研究提供技术基础。  相似文献   

花生高油酸育种研究进展   总被引：7，自引：0，他引：7  

迟晓元  陈明娜  潘丽娟  陈娜  王通  王冕  杨珍  禹山林 《花生学报》2014,(4):32-38

花生是我国重要的油料作物、经济作物和出口创汇作物。花生籽粒油脂的品质是由脂肪酸的组成决定的,高油酸的花生油脂稳定性好,有更高的市场价值与营养价值。因此选育高油酸的花生材料和品种已成为目前花生品质改良育种的重要内容。本文综述了高油酸含量性状的遗传规律、高油酸产生的分子机制、杂交育种、突变育种、基因工程育种等方面的研究进展。分析高油酸花生育种中存在的问题,以期为我国花生高油酸育种和种质资源的发掘与利用提供参考。  相似文献   

花生中UDP-glucosyltransferase基因的克隆及在非生物胁迫下的表达研究     

陈娜  胡东青  潘丽娟  迟晓元  陈明娜  王通  王冕  杨珍  禹山林 《中国油料作物学报》2014,36(3):308

通过RACE技术从花生叶片cDNA中克隆到了UDP-glucosyltransferase基因,命名为AhUGT83A1-like （Genbank注册号为KF411463）。该基因全长为1 530bp,ORF为1 380bp,编码460个氨基酸。序列比对与进化分析结果表明,该序列有保守的UDPGT结构域,并且与其它植物的UGT蛋白同源性较高。荧光定量PCR结果表明,AhUGT83A1-like基因在高盐和低温处理的花生根和叶片中均上调表达;在干旱处理时,根中表达也受到显著上调,但叶片中表达量则有明显下降。以上结果表明AhUGT83A1-like可能参与了花生对干旱、低温和高盐的抗性调控。ABA处理结果表明,AhUGT83A1-like在花生根中对ABA响应明显,但在叶片中对ABA没有明显响应,表明该基因在花生根中对非生物胁迫的调控可能是以依赖ABA的方式发挥作用的。?  相似文献   

Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro     

Jun-Xiu Liu  Min-Qi Luo  Meng Xia  Qi Wu  Si-Mei Long  Yaohua Hu  Guang-Chun Gao  Xiao-Li Yao  Mian He  Huanxing Su  Xiong-Ming Luo  Shu-Zhong Yao 《Marine drugs》2014,12(5):2790-2801

Angiogenesis is the formation of blood vessels from pre-existing vasculature. Excessive or uncontrolled angiogenesis is a major contributor to many pathological conditions whereas inhibition of aberrant angiogenesis is beneficial to patients with pathological angiogenesis. Catunaregin is a core of novel marine compound isolated from mangrove associate. The potential anti-angiogenesis of catunaregin was investigated in human umbilical vein endothelial cells (HUVECs) and zebrafish. HUVECs were treated with different concentrations of catunaregin in the presence or absence of VEGF. The angiogenic phenotypes including cell invasion cell migration and tube formation were evaluated following catunaregin treatment in HUVECs. The possible involvement of AKT, eNOS and ERK1/2 in catunaregin-induced anti-angiogenesis was explored using Western blotting. The anti-angiogenesis of catunaregin was further tested in the zebrafish embryo neovascularization and caudal fin regeneration assays. We found that catunaregin dose-dependently inhibited angiogenesis in both HUVECs and zebrafish embryo neovascularization and zebrafish caudal fin regeneration assays. In addition, catunaregin significantly decreased the phosphorylation of Akt and eNOS, but not the phosphorylation of ERK1/2. The present work demonstrates that catunaregin exerts the anti-angiogenic activity at least in part through the regulation of the Akt and eNOS signaling pathways.  相似文献