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A 66 kDa adult Haemonchus contortus excretory/secretory (E/S) antigen was identified in Western blot by reaction with sera from the infected goats. The protein was purified from the adult worm extract and E/S products by anion exchange and ConA-Sepharose chromatography. The purified protein inhibited monocyte function in vitro as judged by decreased production of hydrogen peroxide and nitric oxide in the culture medium. The protein also caused proliferation of peripheral blood mononuclear cells. The absence of protein in the free living L3 larvae suggests that the expression of this protein coincides with the adaptation to the parasitic life. A correlation of antibody titre and worm burden was observed in the infected goats with higher antibody levels in high worm burdened animals. Anti-protein antibody caused loss of adult worm motility in vitro resulting in the death of the parasite. The fact that the protein is recognized by the host together with in vitro killing of adult parasites by antibodies makes this protein a promising candidate for vaccination trial. 相似文献
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This article elucidates the effect of crude dietary aflatoxin B1 (AFB1) on the stability of the lysosomal membrane in tilapia (Oreochromis mossambicus), and the analysis was made at the biochemical and cellular level. The liver lysosomal acid phosphatase activity was used to evaluate the functional integrity of the lysosome. Three experimental groups were fed on diets containing 0.375, 2.5 and 6 mg kg?1 of AFB1 for a time period of 6 weeks, whereas a fourth group fed on the semi‐purified diet formed the control. Significant changes were observed in the stability of the lysosomal membrane in aflatoxin exposed groups. This is the first report, so far, of changes induced by AFB1 on the stability of lysosomal membrane in tilapia (O. mossambicus). 相似文献
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Suchitra Banerjee Sailendra SinghHarshita Pandey Pallavi PandeyLaiq ur Rahman 《Industrial Crops and Products》2012,36(1):383-388
Curcuma amada Roxb. (family Zingiberaceae), is gaining global attention as potential source of new drug molecule(s) as it is credited with compounds having remarkable pharmacological properties. Recognizing the risk of loss of genetic variability of C. amada in response to imprudent exploitation of the natural resources coupled with unsystematic cultivation practices, preservation of its genetic resources has attracted the current research attention. The present study exploited the multifarious properties of Luffa sponge (i.e. sound physical strength, stability in texture/shape over wide pH range and repeated autoclaving, cost effective availability, etc.) as a novel matrix for synthetic seed conservation. In this study, we have evaluated the consequences of the presence of two fungicides, namely - bavistin and rosebengal, in the gel matrix as well as in the Luffa sponge for conservation of contamination free germplasm of C. amada. A maximum of 50% and 75% regrowth could be recorded amongst rosebengal and bavistin containing synthetic seeds respectively after 4 weeks of plating on plant growth regulator-free MS medium. Further growth of the encapsulated microshoots was significantly impeded by the presence of rosebengal within the synthetic seeds. On the contrary, the bavistin containing synthetic seeds demonstrated five times better regeneration ability to contamination-free shoots. A total of 78% survival was achieved upon transfer of these healthy plantlets to glass-house. RAPD fingerprinting revealed 84.62% genetic similarity between randomly selected synthetic seed derived plantlets. This report strengthened the vital conservation approach of C. amada using inexpensive Luffa sponge as storage matrix and bavistin for eradication of contaminations. 相似文献
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Gauri Saxena Praveen Chandra Verma Suchitra Banerjee Sushil Kumar 《Industrial Crops and Products》2008,27(1):86-90
Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium graveolens L’Her Ex Ait. cv. Hemanti were successfully transferred to the field in Northern India for evaluation. Two distinct morphotypes were described on the basis of leaf dentation-one resembling the parental cultivar in having highly dentated leaves (HDL) and the other with less dentated leaves (LDL). After repeated field-testing for 3 consecutive years, the HDL clones closely resembled the parental cultivar with respect to the different quantity and quality determining traits, while the LDL group was clearly different. The field established LDL somaclones possessed higher herb yield, number of branches and other oil yield attributing traits as compared to the HDL clones and the parent cultivar. The chemical investigations of the essential oil revealed significant differences between the LDL clones, the HDL clones and the control. Selection of such somaclones, which are superior to the parental in most of the quantitative and qualitative traits and show better adaptability to different areas of cultivation, will help towards commercialization of geranium in India. 相似文献
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