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The purpose of the present study was to investigate the toxicity of cryoprotectants on the hatching rate of rainbow trout (Oncorhynchus mykiss) embryos. Epiboly and first eye pigmentation stage embryos were immersed in six permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), propylene glycol (PG), ethylene glycol (EG), and acetamide (Ac), in concentrations of 1–5 M for 5 or 10 min and two non-permeable cryoprotectants, sucrose (Suc) (10%, 15%, 20%) and polyvinyl pyrrolidone (PVP) (5%, 10%, 15%) for 5 min. The embryos were then washed and incubated until hatching occurred. The toxicity of the cryoprotectant was assessed by the hatching rate. The results illustrated that permeable cryoprotectant toxicity for rainbow trout embryos increased in the order of PG < DMSO < MeOH < Gly < EG < Ac. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. There were no significant decreases in hatching rate of embryos treated with sucrose and PVP with the increase of concentration; sucrose had higher hatching rates than PVP. Rainbow trout embryos at first eye pigmentation stage exhibited greater tolerance to cryoprotectants than embryos at epiboly stage. 相似文献
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A. Yousefi Jourdehi M. Sudagar M. Bahmani S. A. Hosseini A. A. Dehghani M. A. Yazdani 《Fish physiology and biochemistry》2014,40(1):117-128
Oocyte maturation in fish is a hormonally regulated process. In the light of long-term oocyte maturation in beluga, the aim of this research was to study the estrogenic effects of different concentrations of soy dietary genistein (GE) and equol (EQ) on the growth performance and ovary development in farmed female Huso huso. Fish were fed with concentrations 0 (control), 0.2, 0.4, 0.8 and 1.6 g of EQ and GE per kg of isoproteic (CP 45 %) and isoenergetic (19.5 MJ/kg) diets during a year. Blood samples and ovary biopsies were collected from each fish seasonally. The main results of the present experimentation are that growth performance was not affected significantly both in GE and EQ (P > 0.05). EQ at concentration 0.4 g/kg had more estrogenic effects than other concentrations of EQ and GE in beluga so that 64 % of fish were matured sexually. Some reproductive indices such as oocyte diameter, testosterone (T) and 17β-estradiol (E2) increased significantly at EQ 0.4 g/kg at the end of experiment (P < 0.05), while 17α-hydroxy progesterone level (17α-OHP) showed no significant changes at all concentrations. Biochemical indices such as calcium, phosphorous and cholesterol increased at GE concentrations, but decreased at EQ concentrations similarly at the end of experiment. There was a negative relationship between plasma phosphorous and alkaline phosphatase enzyme levels. Based on results, EQ at concentration 0.4 g/kg improved oocyte development more than the other concentrations of GE and EQ, and therefore, it can be used as an additive to diets for inducing ovary development in this species. 相似文献
3.
Keivanloo Saeide Sudagar Mohammad Hajibeglou Abasali Shakiba Moradmohammad 《Aquaculture International》2022,30(1):399-408
As a study of cryoprotectant toxicity is an essential prerequisite for the development of a cryopreservation protocol, this study focuses on determining the toxicity of four permeable cryoprotectants: dimethyl sulfoxide (DMSO), propylene glycol (PG), methanol (MeOH), and acetamide (Ac). In cryoprotectant toxicity experiments, striped gourami (Trichogaster fasciata) embryos at three different developmental stages (multi cell, 100% epiboly, and proliferation of somites) were exposed to cryoprotectant solutions with concentrations from 1 to 4 M for a period of 5 and 15 min. Following these treatments, the embryos were incubated until the evaluation of hatching rate. Embryos were tolerant to low concentrations of all cryoprotectants tested in the range of 1 to 2 M for all developmental stages. Early stage embryos were more vulnerable to high concentration (3 and 4 M) than late stage embryos. Results also showed that as concentration and duration of exposure increased, the hatching rate significantly decrease (P < 0.05). On a molar-equivalent basis, DMSO appeared to be less toxic to PG, MeOH, and Ac in general. Exposure to cryoprotectants revealed a stage-dependent sensitivity. Toxicity increased in the order of MeOH < DMSO < PG < Ac in multi cell stage and DMSO < MeOH < PG < Ac in 100% epiboly and proliferation of somites stages. The proliferation of somites stage embryos was less sensitive to exposure to cryoprotectants than multi cell and 100% epiboly stages. These findings could be important for designing cryopreservation protocols for this demanding ornamental species.
相似文献4.
Asad Mohammadi Zarejabad Mohammad Ali Jalali Mohammad Sudagar Somayeh Pouralimotlagh 《Fish physiology and biochemistry》2010,36(3):655-659
The effect of environmental salinity on hematological parameters of great sturgeon Huso huso juveniles was studied. Five-month-old juveniles (mean body weight 28.3 ± 2.1 g) were subjected to 0, 3, 6, 9, and 12 ppt
salinities. The hematological parameters were assessed after a period of 20 days rearing at these salinities. After transfer
from fresh water to brackish water, red blood cells, hematocrit, haemoglobin and mean corpuscular haemoglobin decreased, but
mean corpuscular volume increased. Mean corpuscular haemoglobin concentration, white blood cells, monocyte counts, and eosinophil
counts showed no significant variations with increase in environmental salinity. An increase was found in lymphocyte counts
according to the increase of salinity from 0 to 12 ppt, while the fresh water control group maintained basal levels. Decrease
in neutrophil counts was observed in great sturgeon with increase in environmental salinity. These data show significant effect
of salinity on the blood parameters of great sturgeon. 相似文献
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Mohammad Ali Jalali Ehsan Ahmadifar Mohammad Sudagar & Ghobad Azari Takami 《Aquaculture Research》2009,40(7):804-809
Growth performance, carcass quality, survival and haematological responses were determined when Huso huso juvenile (41.7±1.8 g) fed diets containing Ergosan (an algal product) at 0, 2.0, 4.0 and 6.0 g kg−1 for 60 days. Each diet was fed to triplicate groups of fish at 10-day intervals (1–10, 20–30 and 40–50 with non-supplemented diets and 10–20, 30–40 and 50–60 with supplemented diets). Results showed that fish fed diets containing Ergosan had significantly higher growth than the control group ( P <0.05). Survival was not different among all dietary treatments ( P >0.05). Food conversion ratio in the fish fed a diet containing 4.0 and 6.0 g kg−1 Ergosan was significantly better than the other treatments ( P <0.05), whereas protein efficiency ratio was not different between experimental diets. Lymphocyte count in the fish fed diets containing Ergosan was higher than the other treatments. Haematocrit, haemoglobin (Hb) concentration, number of erythrocytes, total leucocytes, monocyte, eosinophil, myelocyte, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were not different between treatments. Neutrophil count in the control group was higher than the fish fed supplemented diets. Furthermore, whole body lipid, moisture and fibre were not different among dietary treatments ( P >0.05) but body protein in the fish fed a diet containing Ergosan at the level of 2.0 and 4.0 g kg−1 was higher than the other treatments. Whole body ash content was higher in the control group. It was concluded that dietary administration of Ergosan can influence some growth and haematological parameters in great sturgeon, H. huso juveniles. 相似文献
6.
Effect of dietary soybean meal and multienzyme on intestine histology of beluga sturgeon (Huso huso)
International Aquatic Research - This study aimed to evaluate the effect of soybean meal and multienzyme supplementation on intestinal histopathology of beluga sturgeon (Huso huso). Nine diets were... 相似文献
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Ebrahimi G Ouraji H Khalesi MK Sudagar M Barari A Zarei Dangesaraki M Jani Khalili KH 《Journal of animal physiology and animal nutrition》2012,96(4):591-599
This study evaluated the effects of a commercial prebiotic, Immunogen, on feed utilization, growth rate, immunity promotion and carcass composition of the common carp Cyprinus carpio fingerlings. The fingerlings were adopted for 2 weeks and then reared in triplicate groups in 250-l tanks (n = 15 per tank with average initial weights of 11.12 ± 0.55 g). The fish fed on five isonitrogenous and isoenergetic experimental diets containing different levels of Immunogen (0, 0.5, 1, 1.5 and 2.5 g prebiotic/kg diet) to apparent satiation thrice a day for 8 weeks. Weight gain showed no differences among the groups fed different Immunogen levels. Both feed efficiency ratio and protein efficiency ratio significantly (p < 0.05) increased with increasing Immunogen levels from 0.5 to 1.5 g/kg diet. The highest protein content (p < 0.05) was found in the fish fed a diet containing 2.5 g/kg prebiotic. Haematological parameters and plasma total protein concentration were also significantly higher (p < 0.05) in the fingerlings fed diets containing 1.5 and 2.5 g/kg prebiotic in relation to the control. The control fish contained the highest mean of total bacterial counts. The lowest mean (p < 0.05) of total bacterial counts was observed in the fish fed the diet containing 2.5 g/kg Immunogen. The present study reveals that a dietary Immunogen supplementation from 1 to 1.5 g/kg is capable to improve the feed efficiency and growth performance of C. carpio fingerlings as well as their resistance to A. hydrophila infection. 相似文献
8.
Asad Mohammadi Zarejabad Mohammad Sudagar Mohammad Reza Imanpour Valiolah Jaafari 《Journal Of Applied Aquaculture》2013,25(3):207-211
We examined the influence of different hatching times on growth and survival of beluga, Huso huso, over a 28-day period. Larvae from a single pair of beluga, Huso huso, were divided according to hatching time into four groups: early, mid (between 6–9 h after the first group), late (23–26 h after the first group), and a mixture of first to last hatched larvae. At hatching time, there was no statistical difference (P > 0.05) in size and length among the four groups. Prior to the onset of exogenous feeding, groups A and D exhibited slightly earlier onset of aggregation behavior and began exogenous feeding 18 h sooner than the other groups (P < 0.05). Mortality after the onset of the endogenous feeding phase was not significantly different among groups (A: 8.15% ± 0.47%; B: 8.55% ± 0.41%; C: 6.63% ± 0.53% and C: 8.23% ± 0.43%) (P > 0.05). Results showed that hatching time has no effect on the growth and overall mortality of larval beluga. 相似文献
9.
Mohammad Sudagar Abbas Hajibaglo Hamed Kolangi Miandare 《Journal Of Applied Aquaculture》2013,25(1):81-87
This study was carried out to determine the effect of feeding time on growth parameters, food conversion ratio, and survival rate of Huso huso fingerlings (2.11 ± 0.03 g) over a period of five weeks. Fish in triplicate tanks were fed a commercial pelleted diet four times every 24 h on one of four schedules: at 08:00, 11:00, 14:00, and 17:00 h (day feeding); at 08:00, 12:00, 18:00, and 22:00 h (day/night feeding); at 18:00, 21:00, 24:00, and 06:00 h (night feeding); or at 08:00, 12:00, 18:00, and 22:00 h (full dark feeding). At the end of the experimental period, fish in the day-fed group had significantly (P < 0.05) better final weight, daily growth rate, specific growth rate, survival, condition factor, and food conversion ratio than day/night and night-fed groups. Performance of fish fed under full dark conditions was intermediate between day-fed and the other groups. In terms of rearing costs, daytime feeding of Huso huso is the most efficient. 相似文献
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