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排序方式: 共有198条查询结果,搜索用时 19 毫秒
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本研究利用同源克隆法以苹果属平邑甜茶和杂种后代叶片为试验材料,克隆得到4个SERK基因家族片段,并分析了SERKs家族基因在平邑甜茶和杂种后代不同器官和组织中的表达情况。研究结果表明:分离获得的4个SERK基因家族片段的氨基酸序列与模式植物拟南芥、烟草等植物的同源性均在84%以上,同时与其他物种的氨基酸序列进行Blast比对都具有较高的同源性;RT-PCR定量分析结果表明SERK1、SERK2、SERK3、SERK4基因主要在生殖器官中表达。因此,推测SERKs基因在平邑甜茶和杂种后代生殖发育过程中起着重要的调控作用。本研究结果为进一步揭示无融合生殖的发生机理奠定了基础。 相似文献
3.
S. Shanmugam K. H. Kjaer C.‐O. Ottosen E. Rosenqvist D. Kumari Sharma B. Wollenweber 《Journal of Agronomy and Crop Science》2013,199(5):340-350
This study analysed the alleviating effect of elevated CO2 on stress‐induced decreases in photosynthesis and changes in carbohydrate metabolism in two wheat cultivars (Triticum aestivum L.) of different origin. The plants were grown in ambient (400 μl l?1) and elevated (800 μl l?1) CO2 with a day/night temperature of 15/10 °C. At the growth stages of tillering, booting and anthesis, the plants were subjected to heat stress of 40 °C for three continuous days. Photosynthetic parameters, maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm) and contents of pigments and carbohydrates in leaves were analysed before and during the stress treatments as well as after 1 day of recovery. Heat stress reduced PN and Fv/Fm in both wheat cultivars, but plants grown in elevated CO2 maintained higher PN and Fv/Fm in comparison with plants grown in ambient CO2. Heat stress reduced leaf chlorophyll contents and increased leaf sucrose contents in both cultivars grown at ambient and elevated CO2. The content of hexoses in the leaves increased mainly in the tolerant cultivar in response to the combination of elevated CO2 and heat stress. The results show that heat stress tolerance in wheat is related to cultivar origin, the phenological stage of the plants and can be alleviated by elevated CO2. This confirms the complex interrelation between environmental factors and genotypic traits that influence crop performance under various climatic stresses. 相似文献
4.
Dietary bovine lactoferrin induces changes in immunity level and disease resistance in Asian catfish Clarias batrachus 总被引:2,自引:0,他引:2
The effects of including bovine lactoferrin (Lf) in the diet of the Asian catfish (Clarias batrachus) on specific and non-specific immunity as well as disease resistance were investigated. The catfish were fed four different diets for 2 weeks: a commercial diet as control and the same diet supplemented with 50, 100 and 200mg bovine Lf/kg feed. After 1 and 2 weeks, serum bacterial agglutination titre against Aeromonas hydrophila as a measure of specific immunity; natural serum haemolysin titre, lysozyme activity and oxidative radical production by neutrophils as a measure of non-specific immunity as well as disease resistance against A. hydrophila challenge to vaccinated and non-vaccinated animals were evaluated. The results showed that Lf supplements, particularly at 100mg level, significantly (P<0.05) enhanced serum lysozyme level, oxidative radical production and level of protection against A. hydrophila challenge in non-vaccinated animals irrespective of length of exposure. The specific immunity was not influenced by Lf feeding as evidenced from the bacterial agglutination titre and level of protection in vaccinated animals. As Lf feeding at 100mg/kg for 1 week is able to enhance the non-specific immunity and disease resistance of catfish efficiently, these results support the possible use of Lf as an immunostimulant for farmed catfish. 相似文献
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Bordoloi GN Kumari B Guha A Thakur D Bordoloi M Roy MK Bora TC 《Pest management science》2002,58(3):297-302
Screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, Streptomyces sp 201, of a soil. The bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. The antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as Fusarium oxysporum Schlect, F moniliforme Sheldon, F semitectum Berkeley & Ravenel, F solani (Martius) Sacc and Rhizoctonia solani Kuehn. The compound had no effect on seed germination and seedling development as displayed by root and stem growth of the test plant species. In pot experiments with seedlings of cruciferous plants such as Raphanus sativus L (radish), Brassica campestris L (yellow mustard), Brassica oleracea var botrytis L (cauliflower), the antibiotic compound showed promising protective activity of 92% when seeds of the test plants were treated at a dose of 50 micrograms ml-1 prior to sowing. Seed treatment with a spore suspension (3 x 10(8) spores ml-1) of the Streptomyces sp 201 displayed protective activity in the range of 56-60%. Seeds coated with 2.5% methyl cellulose-amended spores of the antagonist showed protective activity in the range of 64-72%. Further, seed treatment with the culture filtrate of the antagonist also showed promising protective activity in the range of 64-84%. 相似文献
7.
Zaluzanin D, a sesquiterpene lactone has been isolated from the aerial parts of Vernonia arborea as a major constituent (0.04%). Antifungal activity of zaluzanin D has been studied against six plant-pathogenic fungi. 相似文献
8.
Rashmi Kumari Hirokazu Ito Masahiro Takatani Miho Uchiyama Tadashi Okamoto 《Journal of Wood Science》2007,53(6):470-480
Although wood/cellulose-plastic composites (WPC) of low wood/cellulose content have been more accepted worldwide and are promoted
as low-maintenance, high-durability building products, composites containing high wood/cellulose content are not yet developed
on an industrial scale. In this study, flow properties, mechanical properties, and water absorption properties of the compounds
of cellulose microfiber/polypropylene (PP) and maleic anhydride-grafted polypropylene (MAPP) were investigated to understand
effects of the high cellulose content and the dimensions of the cellulose microfiber. The molding processes studied included
compression, injection, and extrusion. It was found that fluidity is not only dependent on resin content but also on the dimension
of the filler; fluidity of the compound declined with increased fiber length with the same resin content. Dispersion of the
composite was monitored by charge-coupled device (CCD) microscope. Increasing the plastic content in the cellulose-plastic
formulation improved the strength of mold in addition to the bond development between resin and filler, and the tangle of
fibers. The processing mode affected the physicomechanical properties of the cellulosic plastic. Compression-molded samples
exhibited the lowest modulus of rupture (MOR) and modulus of elasticity (MOE) and the highest water absorption, while samples
that were injection-molded exhibited the highest MOR (70 MPa) and MOE (7 GPa) and low water absorption (2%). 相似文献
9.
Antibodies to Coxiella burnetii have been found in the Danish dairy cattle population with high levels of herd and within herd seroprevalences. However, the prevalence of antibodies to C. burnetii in Danish beef cattle remains unknown. The objectives of this study were to (1) estimate the prevalence and (2) identify risk factors associated with C. burnetii seropositivity in Danish beef and dairy cattle based on sampling at slaughter. 相似文献
10.
Dietary levamisole modulates the immune response and disease resistance of Asian catfish Clarias batrachus (Linnaeus) 总被引:2,自引:0,他引:2
In order to determine the immunomodulatory effect of dietary levamisole in Asian catfish (Clarias batrachus), fish were fed four different diets for 10 days: a formulated diet as control and the same diet supplemented with 50, 150 or 450 mg levamisole kg?1 feed. The serum bacterial agglutination titre against Aeromonas hydrophila as a measure of specific immunity, serum haemagglutination titre, natural haemolytic complement activity (ACH50), myeloperoxidase and lysozyme activities, total protein level and oxidative radical production by neutrophils as a measure of non‐specific immunity as well as disease resistance against A. hydrophila challenge to separate vaccinated and non‐vaccinated groups were evaluated at 0, 1, 2 and 3 weeks after last administration of levamisole. Levamisole supplement at the lowest level (50 mg kg?1) significantly enhanced oxidative radical production and serum myeloperoxidase (MPO) content immediately after 10 days of feeding, which reached peak values after 3 and 2 weeks of feeding respectively. Haemolytic complement and haemagglutination titre were significantly enhanced after 3 and 1 weeks respectively. Haemolytic complement activity and MPO activities were significantly raised to 150 mg kg?1 after 3 and 2 weeks, respectively. At the highest level of levamisole feeding (450 mg kg?1) significant decreases in superoxide production and complement activity were measured immediately after levamisole feeding, which returned to the normal level after 1 week post‐ feeding. Fish were challenged with a virulent strain of A. hydrophila at 0, 1, 2 and 3 weeks after levamisole feeding, and the cumulative per cent survival was recorded over 10 days. Feeding levamisole at 50, 150 or 450 mg kg?1 increased per cent survival in vaccinated fish immediately after levamisole feeding, and survival was significantly higher at 450 mg kg?1. There was no difference in mortality patterns in non‐vaccinated fish. The results support the use of levamisole at 50 mg kg?1 feed for 10 days as an immunostimulant in Asian catfish farming. 相似文献