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P. Banik 《Journal of Agronomy and Crop Science》1996,176(5):289-294
A field experiment was conducted during the winter season of 1992–93 and 1993–94 at Agricultural Experimental Farm, Giridih, Bihar to evaluate the intercropping systems of legumes, gram ( Cicer arietinum L.), pea ( Pisum sativum ) and lentil ( Lens culinaris ) with wheat ( Triticum aestivum ) in 1:1 and 2:1 'row replacement series'. Intercropping systems were assessed on the basis of new indices termed as actual yield loss (AYL) and intercropping advantage (IA). This paper highlights the comparative effectiveness of evaluating the intercropping systems through the existing intercropping indices like LER, RCC, aggressivity. The indices AYL and IA seem to be more appropriate particularly when per plant yield is considered. Intercropping reduced the yield of component crops compared with respective pure stands. Wheat + pea in 1:1 row replacement series gave the highest wheat yield equivalent value (3.02 t ha−1 ) followed by wheat + lentil (2.91 tha−1 ). When the actual sown proportion was considered wheat + lentil (1:1) resulted in maximum AYL (+0.610) and IA (+0.279) values. This treatment also gave the maximum monetary advantage (Rs 5985.45 ha−1 ). 相似文献
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A new index has been proposed for assessing an intercropping system where the component crops are in row replacement senes. It determines the Actual Yield Loss or gain (AYL) in respect to a component crop in an intercropping situation Here the sown proportion of the component crops with regard to its sole one is to be considered. This index appears to be more appropriate than other established indices like land equivalent ratio, relative yield loss, etc., particularly when per plant yield is considered. Partial AYLs (which are the two components of this index) can be considered as indicators regarding the competition existing between the component intercrops. 相似文献
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Polycyclic aromatic hydrocarbons(PAHs) in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium(named as NCPR), composed of Stenotrophomonas acidaminiphila NCW702,Alcaligenes faecalis NCW402, Pseudomonas mendocina NR802, Pseudomonas aeruginosa N6P6, and Pseudomonas pseudoalcaligenes NP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances(EPS), and catechol2,3-dioxygenase(C23O) activity, were evaluated. P. aeruginosa N6P6 and P. pseudoalcaligenes NP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration(extracted from all the 5 selected isolates). Significantly(P 0.001) high phenanthrene(70.29%) and pyrene(55.54%) degradation was observed in the bioaugmented soil microcosm. The C23O enzyme activity was significantly(P 0.05) higher in the bioaugmented soil microcosm with phenanthrene added at 173.26 ± 2.06 nmol min~(-1) mg~(-1) protein than with pyrene added at 61.80 ± 2.20 nmol min~(-1) mg~(-1) protein. The C23O activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation. 相似文献
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A. Midya K. Bhattacharjee S. S. Ghose P. Banik 《Journal of Agronomy and Crop Science》2005,191(3):195-201
Field experiments were conducted at Agricultural Experimental Farm, Giridih, Jharkhand, during the kharif (wet) seasons of consecutive four years (1998–2001) to study the effect of staggered seeding of blackgram (Phaseolus mungo L.) in rice field as a cereal–legume intercropping system and to minimize the degree of competition between the crop species to avoid yield damage, and at the same time to create a high level of competition by the intercrops to suppress the weeds. Intercropping systems were assessed on the basis of land equivalent ratio, relative crowding coefficient, aggressivity, actual yield loss, monetary advantage, etc. Intercropping reduced the yield of component crops when compared with respective pure stands. However, deferred seeding of blackgram in rice (30 cm) after one weeding was most remunerative system and registered maximum rice-equivalent yield (2711 kg ha−1). Rice–blackgram (20 cm) intercropping system was very effective for weed smothering among unweeded intercropping treatments. In conclusion, deferred seeding of blackgram in rice field (30 cm) with one weeding may be recommended for better yield, weed suppression and better economics in the eastern plateau region of India. 相似文献
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Velmurugan GV Tewari AK Rao JR Baidya S Kumar MU Mishra AK 《Veterinary parasitology》2008,154(3-4):185-192
Toxoplasmosis, caused by Toxoplasma gondii, is a disease of economic importance in livestock, especially in sheep and goats, where it causes abortion. Although several serological tests are in use for diagnosis of infection, production of reliable reagents is a constraint. An 814 bp sequence coding for a truncated surface antigen surface antigen 1 (SAG1), a tachyzoite stage-specific protein, as well as a 657 bp sequence coding for granule protein 7 (GRA7), a dense granule protein were PCR amplified from the genomic DNA of T. gondii. The amplified products were ligated in pET-32b(+) and pET-32c(+) expression vectors, respectively and subsequently transformed into BL21(DE3)pLysS cells. A high-level expression of the histidine-tagged SAG1 and GRA7 fusion proteins were obtained after 7h of incubation. The recombinant proteins were purified using Ni-NTA column and were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis using reference positive sera from goat, rabbit and humans at 1:100 dilution. Subsequently, the diagnostic efficiency of the recombinant proteins, either individually or as a cocktail of the recombinant proteins, was assessed with 56 reference goat sera by enzyme-linked immunosorbent assay (ELISA). The immunoreactivity of the refolded SAG1 and GRA7 was evidenced by high OD values. The reactivity of the recombinant proteins as a cocktail preparation was more than that of individual proteins in ELISA and could detect accurately the infection in goats. This is the first report of serological detection of caprine toxoplasmosis by ELISA using a cocktail of recombinant Toxoplasma proteins. 相似文献
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Lantana camara is an ornamental plant used in traditional medicine for the treatment of various diseases. The roots of L. camara is a rich source of oleanolic acid which has shown anti-inflammatory, hepatoprotective, antitumor, antioxidant and anti-hyperlipidemic activity. Optimization of various extraction parameters using response surface methodology (RSM) was performed to assess maximum yield of oleanolic acid from L. camara roots. Plackett–Burman design criterion was applied to identify the significant effects of various extraction parameters such as temperature, time, mean particle size, solvent–solid ratio, solvent composition and number of extraction steps on extraction of oleanolic acid. Among the six variables tested extraction time, mean particle size, solvent–solid ratio and solvent composition were found to have significant effect on oleanolic acid extraction. Optimum levels of the significant variables were determined by using a central composite design. The most suitable condition for extraction of oleanolic acid was found to be a single step extraction at extraction temperature 35 °C, extraction time 55 min, solvent–solid ratio 55:1, mean particle size 0.5 mm and solvent composition 52.5% methanol in a methanol-ethyl acetate mixture. At these optimum extraction parameters, the maximum yield of oleanolic acid obtained experimentally (1.74% dry weight of root) was found to be very close to its predicted value of 1.69% dry weight of root. The mathematical model developed was found to fit well with the experimental data of oleanolic acid extraction. 相似文献
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The study was investigated at Agricultural Experimental Farm, Giridih, India during winter seasons of 2007–2008 and 2008–2009. Plants grown with 100% recommended dose of fertilizer (RDF) [nitrogen (N): phosphorus pentoxide (P2O5): potassium oxide (K2O) = 150:60:60 kg ha?1] + AM + Azospirillum (T15) produced maximum chlorophyll, baby cob, and green fodder yield. Root biomass was highest with application of 150% RDF + arbuscular mycorrhizae (AM) + Azospirillum (T16). Co-inoculated plants produced higher chlorophyll, root biomass resulted higher cob and green fodder yield. Biofertilizers supplied along with chemical fertilizers saved 70, 29, and 33 kg N, P2O5 and K2O per hectare, respectively. Nutrient (NPK) uptake was greatest in T15. Residual soil fertility in terms of NPK was recorded maximum in T16. Although, co-inoculated plots built up higher residual soil fertility as compare to sole inoculation. Nutrients use efficiency and benefit cost ratio were higher due to application of 50% RDF with co-inoculants. T16 was most costly whereas T14 (50% RDF + AM + Azospirillum) was most beneficial. 相似文献