Pathogenicity of halophilic Vibrio harveyi in giant freshwater prawns (Macrobrachium rosenbergii De Man)     

Wilailak Siripornadulsil  Mutjarin Thongserm  Surasak Siripornadulsil 《Aquaculture Research》2014,45(12):1979-1988

In this study, the phenotypic and pathogenic properties in freshwater host were characterized in 14 strains of halophilic Vibrio harveyi isolated from infected marine black tiger shrimp, Penaeus monodon. The lysogenic phenotype was assayed via prophage excision and mitomycin C induction. The bacteria were grouped into two types, corresponding to lysogenic and non‐lysogenic strains. The pathogenicity was determined via direct injection of bacterial cultures into post‐larval juvenile giant freshwater prawns, Macrobrachium rosenbergii De Man. All of the infected prawns showed similar symptoms and inflamed hepatopancreas. The V. harveyi isolates derived from the first‐injected infected prawns were re‐isolated and re‐injected into healthy giant freshwater prawns, in which they retained similar infectivity. Both lysogenic and non‐lysogenic Vibrio spp. showed identical virulence associated with 100% mortality within one day post‐injection. TEM micrographs showed hepatopancreatic nuclear deformation and lipid breakdown caused by lysogenic γ‐hemolytic VL19 and non‐lysogenic β‐hemolytic V33. However, the V33 strain was associated with severely disrupted mitochondria. None of the V. harveyi strains was able to produce a biofilm. Together, our findings indicate that the lysogenic and non‐lysogenic halophilic V. harveyi isolated from marine shrimps may use different virulence factors that are responsible for their pathogenicity in freshwater prawns.  相似文献   

Suppressing weeds in direct-seeded lowland rainfed rice: Effect of cutting dates and timing of fertilizer application     

Suchada Sanusan  Anan Polthanee  Alain Audebert  Surasak Seripong  Jean-Claude Mouret 《Crop Protection》2010

Weeds cause substantive yield losses in rainfed rice, particularly direct-seeded rice (DSR). Two field experiments were conducted in 2005 in north-east Thailand to examine the effects of cutting date and timing of fertilizer application on suppressed weed, growth and yield of DSR. Treatments consisted of two fertilizer applications to the main plots (top-dressing at the date of cutting and 15 days after cutting; DAC) and four cutting dates of the sub-plots. Rice and weed cutting was performed at 30, 45, 60 and 75 days after seeding (DAS), compared with an untreated control. Cutting resulted in a higher growth rate and improved grain yield of rice compared to the untreated control. Weed cutting at 60 DAS resulted in the greatest leaf area index, which contributed to the maximum number of tiller as well as maximum plant dry mass when observed at 30 DAC. Lower densities and dry weights of weeds at 30 DAC were observed when cutting was done 60 DAS, resulting in a higher grain yield than the other cutting dates. Timing of fertilizer application had no significant effect on the density and dry weight of grasses and broad-leaved weeds, but did have a significant affected on, resulting in the lowest density of sedges at 45 DAC was observed with fertilizer applied at cutting date. Rice grain yield was higher with top-dressing of fertilizer at 15 DAC than with fertilizer applied at cutting date. The cutting treatments resulted in higher net incomes than the untreated control. These results indicate that rice and weed cutting at an appropriate growth stage is an alternative method to chemical weed control for DSR in rainfed riceland.  相似文献   

Rates and timing of phosphorus fertilizer on growth and yield of direct-seeded rice in rain-fed conditions     

Suchada Sanusan  Anan Polthanee  Surasak Seripong  Alain Audebert  Jean-Claude Mouret 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(6):491-499

Abstract

Phosphorus deficiency in soil and erratic rainfall limit rice production in Northeast Thailand. The objective of this study was to investigate rice's response to different rates and times of phosphorus fertilizer. A field trial was conducted in a farmer's field with a split plot design. Main plots were established using three rates: 15, 30, and 60 kg P ha^?1. Five types of sub-plot were established as follows: (1) P fertilizer was applied by banding below the seeds' depth before seeding (BBS), (2) broadcasting and incorporation into the soil before seeding, and (3) topdressing at three different DAS (days after seeding). Fertilizer was spread onto the soil surface at 10, 20, and 30 DAS. Significant grain-yield increases were affected by time or rate of fertilizer application. Grain yields were maximized by a rate of 60 kg P ha^?1. Fertilizer application by BBS produced higher grain yield than did other treatments, giving higher root-length density and shoot growth, phosphorus uptake, and greater phosphorus-use efficiency than did fertilizer applied by broadcasting or delayed topdressing. BBS resulted in the highest phosphorus enrichment in soil volume with a rate of 60 kg P ha^?1. The high solubility of fertilizer in a localized placement may satisfy the seedlings' high initial demand of phosphorus and provide sufficient phosphorus availability to allow high productivity. Based on these data, in regions with low soil phosphorus availability where rainfall is also erratic, the management of sub-seed banding placement at the start of a crop at the rate of 60 kg P ha^?1 is recommended as the optimum phosphorus fertilizer practice.  相似文献