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目的:在胚鼠中脑曲分离神经干细胞,探讨IL-1α诱导胚鼠中脑神经干细胞分化为多巴胺神经元的效能.方法:体外分离、培养小鼠胚胎的中脑神经干细胞,加入IL-1α诱导其分化,通过巢蛋白(Nestin)、神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、酪氨酸羟化酶(TH)免疫组化鉴定,并统计分析其诱导效能.结果:胚鼠中脑的腹侧中脑曲部位分离获得了大量未分化、呈巢状悬浮生长的细胞球,且能分化为神经元和神经胶质细胞;IL-1α能明显提高NSC定向分化为多巴胺能神经元比例(IL-1α组14.3%±0.8%vs对照组2.6%±0.3%,P＜0.05).结论:胚鼠中脑存在有多向分化潜能的神经干细胞和中脑神经干细胞被IL-1α诱导向多巴胺能神经元的分化比例明显升高. 相似文献
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This experiment was conducted to study the effect of different rearing conditions of indoor and outdoor in winter and summer on growth performance and immune function of Heijiao MA chicken.In the winter and summer, 180 Heijiao MA chicken with the age of 35-day-old were chosen and randomly divided into two groups with three replicates per group and 30 chickens per replicate.The Heijiao MA chicken were reared at outdoor and indoor, respectively, and at 49, 63, 77, 91 days the immune organ indexes, serum antibody titers and growth performance were measured.The results showed that:①Whether it was winter or summer, the body weight of Heijiao MA chicken rearing at indoor were higher than that of outdoor, and the difference was significant at experimental stages in winter (P<0.05).There was significant difference only at 77 days old of Heijiao MA chicken in summer (P<0.05), the rest were not significant (P>0.05);②The ADG of outdoor group in summer was higher than that in winter, while the F/G of outdoor group in summer were significantly lower than that in winter (P<0.05).The ADFI and ADG of indoor group were not significantly different between winter and summer (P>0.05).③On immune function, immune organ indexes and ND antibody titer of Heijiao MA chicken of indoor group in winter were higher than outdoor group, while part of immune organ indexes, AI (H5) and ND antibody titers of outdoor group in summer were higher than that of indoor group;And ND and AI (H5) antibody titers in winter were higher than summer.In conclusion, The climate and season were major factors to influence the growth performance and immune function of Heijiao MA chicken. 相似文献
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为探究不同季节室内、外饲养方式对黑脚麻鸡生长性能和免疫功能的影响,本试验在冬季、夏季各选用180只35日龄的黑脚麻鸡雌雏,随机分成2组,每组3个重复,每个重复30只,分别进行室外放养和室内平养。在49、63、77、91日龄分别对黑脚麻鸡生长性能、免疫器官指数和血清抗体效价进行测定。结果显示,①无论是冬季还是夏季,试验期室内平养的黑脚麻鸡体重均大于室外放养,冬季两试验组均差异显著(P<0.05),夏季仅在77日龄时差异显著(P<0.05),其余日龄差异均不显著(P>0.05);②夏季室外放养组黑脚麻鸡平均日增重(ADG)高于冬季室外放养组,料重比(F/G)显著低于冬季室外放养组(P<0.05),而室内平养组平均日采食量(ADFI)和ADG在两个季节间无显著差异(P>0.05);③在免疫功能上,冬季室内平养黑脚麻鸡免疫器官指数和新城疫(ND)抗体效价均大于室外放养组,而夏季室外放养组部分免疫器官指数和禽流感H5亚型(AI(H5))、ND抗体效价高于室内平养组;冬季两种抗体效价均高于夏季。综上所述,气候和季节是影响放养黑脚麻鸡生长性能及免疫功能的主要因素。 相似文献
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本研究采用盆栽实验,通过模拟添加不同水平的EDTA,强化小蓬草修复Cd及Pb-Cd复合污染的土壤.分析了小蓬草对Cd的吸收特性及EDTA对其转运、富集Cd的影响.结果表明,当EDTA的添加水平为5.0 mmol·kg-1时,单一Cd处理下的小蓬草地上部分的Cd含量达到最大值,比对照提高了70.4％;在Pb-Cd复合污染处理下,EDTA的添加水平为2.5 mmol·kg-1时,小蓬草地上部分Cd含量及其转运系数、富集系数均达到最大值,比较对照增加了535％、89％、1464％. 相似文献
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(一)红壤性水稻土中铁、锰的含量以淀积层最高,潜育层最低。铁、锰在土层中的分化程度,随土壤发育的程度而增加;锰的分化趋势,较铁更为明显。(二)能被氢醌还原的锰量,占全锰中的很大百分数,最高者可达70%,而能被氢醌还原的铁量,不超过全铁量的0.5%。能与EDTA形成络合物的锰量,也较铁量为高。(三)土壤经交互干湿处理以后,酸溶性和易于还原的铁、锰数量都大大降低,说明脱水作用可使铁、锰的活动性减低。(四)作者根据试验结果而认为,锰对氧化还原条件的感应较为灵敏,而铁则对酸碱条件较为敏感,这对于水稻土剖面中铁、锰的移动和分化具有一定意义。铁、锰的移动程度,在一定程度上可作为水稻土发育程度的一个指标。 相似文献
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DENNIS TING DVM JULIEN B. CABASSU DVM REUNAN P. GUILLOU Doc. Vét. MICHAEL T. SINNOTT MS ERIC G. MEYER MS ROGER C. HAUT PhD LOÏC M. DÉJARDIN DVM MS Diplomate ACVS & ECVS 《Veterinary surgery : VS》2009,38(7):881-887
Objective— To investigate the effect of fracture configuration on the mechanical properties of standard interlocking nails (ILNs) and a novel angle-stable ILN (ILNn) in bending.
Study Design— In vitro experimental study.
Sample Population— Synthetic tibial gap fracture bone models.
Methods— Bone models, featuring a 5 or 120 mm central defect, respectively, mimicking a simple diaphyseal and a comminuted fracture involving both metaphyses, were implanted with 6 or 8 mm screwed or bolted standard ILNs (ILN6s, ILN6b, ILN8s, ILN8b, respectively) or an ILNn. Specimens were tested in 4-point bending. Construct angular deformation (AD) and slack were statistically compared ( P <.05).
Results— With increasing gap size, standard ILN construct AD increased significantly by ∼27% in ILN8b and by up to 105% in ILN6s. Similarly, standard ILN construct slack significantly increased by ∼33% in ILN8b (from ∼4.2° to ∼5.6°) and by up to ∼130% in ILN6s (from ∼7° to ∼16°). Conversely, there was no difference in the ILNn construct AD (∼4°) regardless of gap size. ILNn AD was the lowest of all groups and occurred without slack.
Conclusions— This study demonstrated that the angle-stable ILNn provided construct stability regardless of fracture configuration, whereas the intrinsic slack of standard ILNs could jeopardize construct stability in a fracture configuration involving the metaphyses.
Clinical Relevance— Use of standard ILNs may be optimal in diaphyseal fractures where circumferential nail/cortical contact could augment repair stability. Conversely, the angle-stable ILNn may represent a reliable fracture stabilization method for diaphyseal fractures as well as fractures involving the metaphyseal regions. 相似文献
Study Design— In vitro experimental study.
Sample Population— Synthetic tibial gap fracture bone models.
Methods— Bone models, featuring a 5 or 120 mm central defect, respectively, mimicking a simple diaphyseal and a comminuted fracture involving both metaphyses, were implanted with 6 or 8 mm screwed or bolted standard ILNs (ILN6s, ILN6b, ILN8s, ILN8b, respectively) or an ILNn. Specimens were tested in 4-point bending. Construct angular deformation (AD) and slack were statistically compared ( P <.05).
Results— With increasing gap size, standard ILN construct AD increased significantly by ∼27% in ILN8b and by up to 105% in ILN6s. Similarly, standard ILN construct slack significantly increased by ∼33% in ILN8b (from ∼4.2° to ∼5.6°) and by up to ∼130% in ILN6s (from ∼7° to ∼16°). Conversely, there was no difference in the ILNn construct AD (∼4°) regardless of gap size. ILNn AD was the lowest of all groups and occurred without slack.
Conclusions— This study demonstrated that the angle-stable ILNn provided construct stability regardless of fracture configuration, whereas the intrinsic slack of standard ILNs could jeopardize construct stability in a fracture configuration involving the metaphyses.
Clinical Relevance— Use of standard ILNs may be optimal in diaphyseal fractures where circumferential nail/cortical contact could augment repair stability. Conversely, the angle-stable ILNn may represent a reliable fracture stabilization method for diaphyseal fractures as well as fractures involving the metaphyseal regions. 相似文献
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我国依据茶树品种农艺性状进行选育的国家级和省级良种已达300多个,但以综合价值为目标的生态经济型品种筛选尚未见报道。以条形绿茶为例,利用德尔菲法和层次分析法（AHP）对24个茶树品种进行综合评价,筛选出适制优质条形绿茶的生态经济型茶树品种。综合专家打分和对其提出的建议进行小组讨论,形成含6个一级指标和19个二级指标的综合评价体系,其中确定指标的两轮德尔菲法专家积极程度分别为82.5%和100%,专家权威程度分别为0.93和0.90,变异系数（CV）分别为0.19和0.16,肯德尔系数（W）分别为0.296（P<0.05）和0.358（P<0.05）。一级指标权重分别为0.364、0.204、0.128、0.108、0.124、0.072;二级指标权重在0.101~0.805（均通过一致性检验）,其中重要性赋值的德尔菲法专家（第三轮）积极程度为100%、专家权威程度为0.91、CV为0.36、W为0.11（P<0.05）。模型综合评价结果表明,龙井长叶、舒茶早、菊花春分别以3.714、3.624、3.581得分位居前三名,是适制优质条形绿茶的生态经济型品种。 相似文献
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产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究 总被引：1，自引：0，他引：1
真菌漆酶可以高效转化多环芳烃(PAHs),因此,产漆酶真菌在PAHs污染土壤修复中极具应用前景。根据漆酶可将愈创木酚氧化为红色物质的特性,成功从土壤中筛选出一株能够分泌漆酶的真菌菌株F-1,初步鉴定该菌为疣孢漆斑菌(Myrothecium verrucaria)。通过Plackett-Burman试验对菌株F-1的产酶能力进行了分析,发现特定培养条件组合可将其酶活提高近300倍,达5628 U L-1,表明F-1的漆酶活性受到环境条件的显著影响。应用菌株F-1对PAHs污染土壤进行了初步修复研究,结果表明,接种F-1对菲、荧蒽、芘、苯并(a)蒽、屈、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,h)蒽、苯并(g,h,i)苝、茚苯(1,2,3-cd)芘等11种PAHs均有不同程度的降解,提示产漆酶真菌在PAHs污染土壤修复中的应用潜力。 相似文献