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Fish Physiology and Biochemistry - The current study investigated the effects of dietary curcumin nanoparticles (C-NPs) on the performance, hemato-biochemical profile, digestive enzymes activities,...  相似文献   
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Summary

Shoot-tip explants were excised from axenic and non-axenic plant cultures of two cumin (Cuminum cyminum L.) landrace lines from Assiut (ASS) and Qina (QIN), Egypt. Explants were cultured on MS (Murashige and Skoog, 1962) medium supplemented with different concentrations of benzyladenine (BA) or kinetin. The two landraces performed similarly throughout the study. Shoot-tip explants from axenic cultures were superior to those prepared from non-axenically grown plants regarding the percentage of explants that produced micro-shoots and the number of micro-shoots that proliferated. The maximum number of excisable micro-shoots was produced on medium with 1 µM BA. Up to 20 micro-shoots per explant were excised from cultures on this medium. The largest number of micro-shoots obtained on medium containing kinetin was five. Most (80–90%) micro-shoots formed roots on medium with 1 µM indole-3-butyric acid (IBA) and 2% (w/v) polyethylene glycol (PEG-6000). The survival rate ex vitro was as high as 70%. A high concentration of BA (4 µM) induced calli, retarded elongation of the micro-shoots and reduced both the number of roots formed on subsequent rooting medium, and plant survival ex vitro. This study supports the feasibility of in vitro cloning of cumin using shoot tips for germplasm collection, conservation and exchange.  相似文献   
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The treatment of domestic wastewater at a temperature of 12–24°C was investigated in an RBC system. The RBC consists of a two stage system connected in series. The system was operated at different organic loading rates (OLR's) and hydraulic retntion times (HRT's) in order to optimize the RBC performance. The overall removal efficiencies for chemical oxygen demand (CODtotal, CODsuspended and CODcolloidal) significantly decreased when decreasing the total HRT from 10 to 2.5 h and increasing the OLR from 11 to 47 g COD/m2.d. However, the effluent quality of CODsoluble remained unaffected. Most of the COD was removed in the 1st stage and nitrification took place in the 2nd stage of the two stage system.The overall nitrification efficiency was 49% at total OLR of 11 gCOD/m2.d. At total HRT's of 10, 5 and 2.5 h, the Escherchia coli (E. coli) concentration was reduced by a value of 1.6, 1.5 and 0.8 log10 respectively. The sludge volume index (SVI) decreased as the OLR increased. However, the SVI of the excess sludge produced in the RBC under different OLR's was always <74 ml/gTS, which indicates vadjust a good settleability. The performance of the single versus two stage RBC operated at the same total OLR of 22 g COD/m2.d and the same HRT of 5.0 h was examined. The results obtained showed that the COD concentration and the E. coli content in the final effluent of a two stage were lower than in the effluent of the single stage RBC. Moreover, the nitrification efficiency in the two stage system was higher comapred to one stage system.  相似文献   
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Tilmicosin was injected subcutaneously to lactating ewes once at a dose of 10 mg kg-1 b.wt. to determine its plasma, milk, urine and ruminal juice concentrations. Tilmicosin could be detected in all those fluids 30 minutes after injection. Milk and urine concentrations were higher than those of plasma and ruminal juice. The drug was detectable in milk, urine and plasma for 9, 4 and 3 days after injection, respectively. No amount of tilmicosin could be detected in ruminal juice 12 hours following administration. The mean peak concentration of tilmicosin in plasma and milk (Cmax) were 1.29 and 9.5 micrograms ml-1 and were obtained at (Tmax) 5.235 and 15.093 hours, respectively. The drug was slowly eliminated from plasma and milk as indicated by its long half-life (t1/2el) of 15.4 and 26.2 hours, respectively. The mean binding of tilmicosin to plasma and milk proteins in vitro was 16.8% and 26.8%, respectively. The drug was not bound to ruminal juice at any extent. The rate of tilmicosin renal clearance revealed that it was correspondingly increased with higher blood concentrations. While creatinine clearance showed no significant change after tilmicosin administration. The ratio (fractional clearance) between tilmicosin renal clearance to creatinine clearance was less than one indicating that the glomerular filtration is the main pathway of elimination through kidneys. The rate of ruminal gas fermentation in ewes was inhibited after subcutaneous injection of tilmicosin at a dose of 10 mg kg-1 b.wt. The tested samples taken at different time intervals from the rumen of ewes showed a subsequent reduction in the rate of fermentation as compared to control samples. The reduction was correspondingly increased with the increase of tilmicosin concentration in ruminal juice and returned to normal thereafter.  相似文献   
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This study evaluated the impact of dietary ginger and liquorice supplementation on growth performance, physiological and histopathological profiles and heavy metal accumulation in Nile tilapia fingerlings. Fish (n = 1,800, 17.5 ± 0.11 g BW) were randomly distributed into four treatment groups in triplicates and received no supplementation (control group), 5 ml aqueous ginger extract/kg feed (ginger group), 4 ml aqueous liquorice extract/kg feed (liquorice group) or 2.5 ml ginger plus 2 ml liquorice aqueous extracts/kg feed (mix group). The ginger‐liquorice mix supply improved the growth performance and feed efficiency (p < .05), increased the haematocrit and haemoglobin (p < .05), leucocytes (p = .108), neutrophils (p = .054), serum total protein (p < .05), albumin (p = .011) and globulin (p = .094) but decreased (p < .05) the blood urea nitrogen and creatinine than feeding liquorice or ginger lonely compared to the control. Heavy metal loads in pond water induced lamellar telangiectasis of gills and necrosis with sloughing of intestinal villi tips. These detrimental effects were alleviated, and the intestinal villus length (p = .041) and crypt depth (p = .069) were increased with liquorice supply. In all treatment groups, heavy metal contents in fish flesh were lower compared to the control. Thus, using ginger and/or liquorice aqueous extracts can decrease heavy metal accumulation in the fish flesh and exert positive effects on growth performance, metabolic profile and the intestinal and gill morphology of Nile tilapia.  相似文献   
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Arid climate has contributed to crops yield production decrement in many regions due to water shortage and soil salinization. The main target of this research is to enhance wheat production under arid climate stresses by utilizing bio-elicitors. Two common wheat assortments i.?e. cv. Gemmiza 10 and Sakha 93 accomplished tolerant to these stresses utilizing bio elicitors include Acremonium coenophiulum, Streptomyces griseus, Trichoderma harzanium, T. viride, Rhodotorula glutinis, Paenibacillus polymyxa, Bacillus subtilis, B. megaterium, Pseudomonas putida, P. fluorescens compared with natural elicitors as methyl jasmonate (MJ), chitosan (CHI), ascorbic acid and putrescein (PUT). Varieties were planted in two progressive seasons in recently reclaimed lands, where two fields were selected the first is located in North Sinai while the second is situated to the south of Behira Governorate. Bio elicitors reduced abiotic stress and enhance physiological characteristic expressed as Proline, soluble carbohydrates %, Chl.a, Chl.b, Chl.a + b; Chl.a/Chl.b; Carotenoids and increased the concentration of Crude protein, N, P and total soluble carbohydrates in wheat grains. Using P. fluorescens exhibted increment in grain yield of wheat plants, cv sakha 93 amounted by 68.3% in Sinai region, while in Behira region the increement reached (45.5%) with P. polymyxa treatment. The response of Gemmiza 10 variety to the Bio-elicitors was less compared to Sakha 93 variety in both regions, where the highest increments due to P. putida treatment were18.9 and 19.7% in Sinai and beheirwa regions, respectively, compared to the control in each region. A remarkable increments of wheat growth, development and extended survival under salt and water limiting and restricting conditions. These findings demonstrate that wheat plants can display improved stress tolerance through bio-elicitors, and recommend that innovation may be useful in reducing effects of climate change and environmental stress on other crops and expanding agricultural production onto marginal lands.  相似文献   
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A 20-year-old gelding was diagnosed with peritonitis and severe reactive mesothelial hyperplasia. Exploratory laparotomy findings were suggestive of a neoplastic etiology; however, additional diagnostics ruled this out and the horse made a full recovery. This report demonstrates the difficulty and value of differentiating between reactive and neoplastic mesothelial processes.  相似文献   
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This study was designed to investigate the effect of calcium and nitrogen application during heat stress on leaf calcium concentration, transpiration rate, membrane thermostability, and biomass accumulation and partitioning. Micropropagated Russet Burbank potato (Solanum tuberosum L). plants were transplanted into 20 L pots containing 1:1 (v/v) soil: perlite and exposed to 30/20C (D/N) temperatures for four weeks (weeks 9–12 after transplanting) in a controlled-environment growth room. The maximum temperature was maintained for 6 hr during the middle of the 14 hr photoperiod. The nutrition treatments were N before stress (NBS), N during stress (NDS) and Ca and N during stress (Ca+NDS). Calcium was supplied as Ca(NO3)2. All treatments received the same total amount of nitrogen. Native soil Ca level without amendment (550 mg Ca/kg soil) was sufficient for potato plant growth under normal temperatures. Plants given Ca and N during heat stress had the highest leaf Ca concentration and transpiration rate during and 2 weeks after conclusion of the heat stress period. When measured after 4 weeks of heat stress, area and fresh and dry weight of the most recently mature leaf was significantly greater in NDS and Ca+NDS plants compared to NBS plants. Cellular membrane thermostability (measured as ion leakage from heat-treated leaf disks) was not affected by any treatment prior to heat stress. However, leaf tissue from Ca+NDS plants exhibited significantly higher membrane thermostability compared to NBS plants after 2 and 4 weeks of heat stress. At harvest, NDS and Ca+NDS plants had significantly higher leaf/stem (fresh weight ratio) values compared to NBS plants. Also, Ca+NDS plants had significantly greater total tuber and biomass values than NBS and NDS plants. Results of this study suggest that some detrimental effects of heat stress on plant growth and stomatal function may be alleviated by Ca and N application during heat stress. The data also suggest that mitigation of heat stress by Ca and N application during heat stress may maintain plant productivity when optimum growing temperatures are restored.  相似文献   
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