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Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines.  相似文献   
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Platynosomum fastosum is a small fluke found in the biliary ducts and gallbladder of cats. Its lifecycle includes the snail Sublima octona as intermediate host, and lizards, toads and geckos as paratenic hosts. Affected cats are usually adult and acquire the parasite by feeding on infected lizards. This parasite occurs across the world but is more frequent in tropical areas. The clinical signs range from none to obstruction of the biliary tract, with hepatic failure and death, reinforcing the necessity of including the liver fluke Platynosomum fastosum in the differential diagnosis of hepatic diseases in cats. This report describes an unusual case of a cat with a polycystic hepatic disease and a severe infestation by Platynosomum fastosum and presents a review of the literature.  相似文献   
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Understanding the reasons why animals of similar performances have different feed requirements is important to increase profits for cattle producers and to decrease the environmental footprint of beef cattle production. This study was carried out aiming to identify the associations between residual feed intake (RFI) and animal performance, nutrient digestibility, and blood metabolites related to energy balance of young Nellore bulls during the finishing period. Animals previously classified as low (n?=?13) and high RFI (n?=?12), with average initial body weight of 398 kg and age of 503 days were used. Cattle were fed a high energy diet and were slaughtered when rib fat thickness measured by ultrasound between the 12th and 13th ribs reached the minimum of 4 mm. A completely randomized design was adopted, being data analyzed with a mixed model that included the random effect of slaughter group, the fixed effect of RFI class, and linear effect of the covariate feedlot time. No differences were found (p?>?0.10) between RFI classes for performance, dry matter, and nutrients intake. However, dry (p?=?0.0911) and organic matter (p?=?0.0876) digestibility tended to be lower, and digestibility of neutral detergent fiber corrected for ash and protein (p?=?0.0017), and total digestible nutrients (p?=?0.0657) were lower for high RFI animals, indicating lesser capacity of food utilization. Difference between low and high RFI animals was also found for blood cortisol at the end of the trial (p?=?0.0044), having low RFI animals lower cortisol concentrations. Differences in the ability to digest food can affect the efficiency of transforming feed into meat by Nellore cattle.  相似文献   
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Several studies have shown the benefit of the use of probiotics in the prevention and treatment of diseases; however, few of them have investigated the effect of probiotics on parasitosis. In this study, the effect of Saccharomyces boulardii on the intensity of infection of mice with toxocariasis was evaluated. The animals were fed with a diet supplemented with S. boulardii for 15 days before inoculation with Toxocara canis eggs and for 2 or 60 days post-inoculation. S. boulardii promoted a reduction of approximately 36% in the average number of recovered T. canis larvae, suggesting that it can be used as an alternative to help control toxocariasis.  相似文献   
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Length–weight relationships were estimated for 21 coastal fish species of the Azores, namely Abudefduf luridus, Bothus podas, Chromis limbata, Coris julis, Diplodus sargus, Echiichthys vipera, Gaidropsarus guttatus, Labrus bergylta, Mullus surmuletus, Phycis phycis, Pomatomus saltator, Sarda sarda, Scorpaena maderensis, Scorpaena notata, Seriola rivoliana, Serranus atricauda, Sparisoma cretense, Sphyraena viridensis, Synodus saurus, Thalassoma pavo and Trachinotus ovatus. Significant length–weight relationships were found for all species. Sexual dimorphism did not affect the length–weight relationships, except in the cases of S. cretense and C. julis. Length–length equations for converting size measurements (standard length (SL) and fork length (FL) to total length (TL)) are also presented for all fish species.  相似文献   
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Reproductive biology and social behaviour of the parrotfish Sparisoma cretense was studied in the Azores Islands, northeast Atlantic, to characterize its spawning season and general reproductive biology in the region, to clarify the species mating system and associated spawning behaviours, and to evaluate the relationship between its reproductive traits and habitat features. S. cretense exhibits a dual mating system where males hold female harems within year-round territories or live in multi-male groups. Group behaviour predominates in smaller size classes, and territoriality in larger size classes. Males mature and become territorial earlier in life than females, and the best territories are held by larger males. The two behavioural modes are usually spatially segregated, with larger territorial fish preferring exposed and deeper reef ledges, and group fish occupying shallower, protected habitats. However, they frequently interact and overlap in space, especially during reproduction in the summer. Our findings indicate a highly competitive system and suggest that high quality spawning sites are important for the reproductive success of both types of fish. Patchiness of habitat along shorelines determines the relative distribution and equilibrium of territorial versus group fish. The complexity of this relationship between social structure and habitat might impact the populations’ productivity, and could influence the success of marine protected areas for this species.  相似文献   
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