Mechanism of selective toxicity: absorption and detoxication of an antibiotic, ascochitine, by sensitive and insensitive fungi     

T Nakanishi  H Oku 《Phytopathology》1969,59(11):1563-1565

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Graft-versus-host reaction (GVHR) in clonal amago salmon,Oncorhynchus rhodurus     

Qin QW  Ototake M  Nagoya H  Nakanishi T 《Veterinary immunology and immunopathology》2002,89(1-2):83-89

The graft-versus-host reaction (GVHR) was demonstrated in a salmonid model system of clonal diploid and triploid amago salmon. Triploid operculum grafts on clonal diploid evoked an acute rejection within 12 days. Grafts exchanged among triploid amago salmon exhibited prolonged survival for 18 days. In contrast, diploid grafts on triploid, and allografts among clonal diploid amago salmon were accepted. A typical GVHR was induced in triploid recipients by intraperitonal injection of head kidney cells from sensitised diploid donors. The clinical signs of graft-versus-host disease (GVHD) were observed in the recipients after 1 week of cell injection as a loss of appetite and appearance of solid faeces, followed by haemorrhage, local swelling of ventral skin and an enlarged spleen. Three of six fish died within 1 month. Water temperature and frequency of sensitisation are critical to induce GVHR. Diploid donors had to be sensitised three times at 20 degrees C to induce the typical GVHR. GVHR was most effectively induced by head kidney cells, followed by peripheral blood leucocytes (PBL) and spleen cells. Ploidy analysis by flow cytometry revealed that the donor head kidney cells greatly increased in the recipient liver, head kidney and spleen, and reached the peak after 9 days of donor cell injection. The results in the present study are quite similar to the findings in ginbuna and ginbuna-gold fish hybrid system, suggesting the presence of T cells in salmonid as well as cyprinid fish.  相似文献   

Uterine disorders diagnosed by ventrotomy in 47 rabbits     

Saito K  Nakanishi M  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):495-497

The type, ages of occurrence, primary complaints, clinical signs and mortality in forty-seven cases of uterine disorders diagnosed by ventrotomy in rabbits were analyzed retrospectively. Endometrial hyperplasia (29.8%) was most frequently observed, followed by uterine adenocarcinoma (21.3%). Tumorous lesions were seen in 46.8% of the cases. The age of occurrence ranged from two years and two months to seven years and six months, with a peak at four to five years of age. The most common primary complaint was bleeding (62.2%), followed by mammary gland abnormality (12.8%) and increased abdominal circumference (10.6%). Physical examinations revealed mammary gland disorders such as mammary cysts in 31.9% of the cases. Uterine disorders were detected by palpation in 15 out of 32 cases with a primary complaint of bleeding. Ultrasonography showed uterine disorders in 21 out of 24 cases, suggesting that ultrasonography could be useful in the diagnosis of uterine disorders. The outcome seemed to be influenced by physical status rather than malignancy of lesions. The mortality was higher in cases with symptoms such as anorexia, emaciation, severe anemia, and dehydration.  相似文献   

A new method for counting of quail leukocytes by flow cytometry     

Moritomo T  Minami A  Inoue Y  Nakanishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1149-1151

An automatic counting method was developed for fish blood cells using a fluorescent dye, 3, 3-dihexyloxacarbocyanine (DiOC (6)(3)), that selectively stain lipid bilayers in living cells. In the present study, the DiOC(6)(3) method was applied to quail (Cotumix cotumix japonica) blood cells. After quail blood cells were stained with DiOC(6)(3), absolute counts and relative proportions of erythrocytes, granulocytes, monocytes, and lymphocytes plus thrombocytes in whole blood were obtained by means of flow cytometry (FC). The number of each cell types by the FC was in good agreement with those counted microscopically. This method will offer new possibilities for routine blood cell counting for avian medicine.  相似文献   

Effect of Garcinia cambogia extract on serum leptin and insulin in mice     

Hayamizu K  Hirakawa H  Oikawa D  Nakanishi T  Takagi T  Tachibana T  Furuse M 《Fitoterapia》2003,74(3):267-273

In this study we examined the effects of 3.3% Garcinia cambogia extract on 10% sucrose loading in mice for 4 weeks. Treatment was found to have no effect on body weight, fat pad weight or serum glucose level. On the other hand, serum total cholesterol, triglycerides, NEFA were observed. Levels of serum insulin and leptin, as well as the leptin/WAT ratio, were lower in the treated mice than in the control. These findings suggested that G. cambogia extract efficiently improved glucose metabolism and displayed leptin-like activity.  相似文献   

Properties of scallop mantle collagen: its content,tissue distribution and thermal behavior     

Shoshi Mizuta  Yuko Nakanishi  Masashi Shiraishi  Yoshihiro Yokoyama  Reiji Yoshinaka 《Fisheries Science》2007,73(6):1353-1361

ABSTRACT:   To obtain fundamental information for the effective use of scallop Patinopecten yessoensis mantle, which is one of the underutilized marine resources. Some properties of collagen contained in the mantle were examined by chemical and histochemical techniques. Collagen content in the mantle varied annually, ranging from 0.98 to 1.72% of wet tissue, 7.7 to 12.6% of dry tissue and 13.5 to 26.5% of total protein, being relatively in high level of collagen content of invertebrate muscles. Collagen fiber was densely distributed in the inner connective tissue matrix of the mantle pallial, in contrast to the inner fold part which was rich in muscle fibers. The collagen contained in the crude collagen fraction (residue after alkali extraction), prepared from the mantle, was revealed to have considerably low solubility on hot-water extraction, constantly less than 20% of the total collagen at the temperatures in the range of 20–90°C.  相似文献   

Chlorantraniliprole application differentially affects adult emergence of Sympetrum dragonflies in rice paddy fields     

Nakanishi  Kosuke  Usio  Nisikawa  Yokomizo  Hiroyuki  Takashima  Tadao  Hayashi  Takehiko I. 《Paddy and Water Environment》2022,20(1):177-183

Paddy and Water Environment - Rice paddy fields are important habitat for many dragonfly species. In Japan, populations of dragonflies inhabiting rice paddies, in particular Sympetrum (Odonata:...  相似文献   

Identification and characterization of multiple <Emphasis Type="Italic">lck</Emphasis> genes in ginbuna crucian carp <Emphasis Type="Italic">Carassius auratus langsdorfii</Emphasis>   总被引：1，自引：0，他引：1  

Kyosuke Araki  Kaori Hirano  Fumio Takizawa  Tadaaki Moritomo  Mitsuru Ototake  Teruyuki Nakanishi 《Fisheries Science》2007,73(5):1017-1024

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Logic circuits with carbon nanotube transistors     

Bachtold A  Hadley P  Nakanishi T  Dekker C 《Science (New York, N.Y.)》2001,294(5545):1317-1320

We demonstrate logic circuits with field-effect transistors based on single carbon nanotubes. Our device layout features local gates that provide excellent capacitive coupling between the gate and nanotube, enabling strong electrostatic doping of the nanotube from p-doping to n-doping and the study of the nonconventional long-range screening of charge along the one-dimensional nanotubes. The transistors show favorable device characteristics such as high gain (>10), a large on-off ratio (>10(5)), and room-temperature operation. Importantly, the local-gate layout allows for integration of multiple devices on a single chip. Indeed, we demonstrate one-, two-, and three-transistor circuits that exhibit a range of digital logic operations, such as an inverter, a logic NOR, a static random-access memory cell, and an ac ring oscillator.  相似文献   

Pavoninins: shark-repelling ichthyotoxins from the defense secretion of the pacific sole     

Tachibana K  Sakaitanai M  Nakanishi K 《Science (New York, N.Y.)》1984,226(4675):703-705

A series of ichthyotoxic and hemolytic steroid aminoglycosides, pavoninins-1, to -6, has been isolated from the defense secretion of the sole Pardachirus pavoninus, and their respective chemical structures have been established by spectroscopic studies and chemical conversions. The pavoninins exert repellent activity against sharks and are considered to be the factors responsible for the predator-repelling property of the sole.  相似文献