Ethical ideology and moral persuasion: personal moral philosophy, gender, and judgments of pro- and anti-animal research propaganda.     

Darcy Nickell  Harold A Herzog 《Society and Animals》1996,4(1):53-64

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Is “milk crust” a transient form of golden retriever ichthyosis?          下载免费PDF全文

Anja Roethig  Kirsti J. M. Schildt  Monika M. Welle  Brett E. Wildermuth  Reto Neiger  Nina Thom 《Veterinary dermatology》2015,26(4):265-e57

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Case–control risk factor study of methicillin-resistant Staphylococcus pseudintermedius (MRSP) infection in dogs and cats in Germany     

Georg Lehner  Monika Linek  Ross Bond  David H. Lloyd  Ellen Prenger-Berninghoff  Nina Thom  Iris Straube  Kristien Verheyen  Anette Loeffler 《Veterinary microbiology》2014

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a highly drug-resistant small animal veterinary pathogen. Although often isolated from outpatients in veterinary clinics, there is concern that MRSP follows a veterinary-hospital-associated epidemiology. This study's objective was to identify risk factors for MRSP infections in dogs and cats in Germany. Clinical isolates of MRSP cases (n = 150) and methicillin-susceptible S. pseudintermedius (MSSP) controls (n = 133) and their corresponding host signalment and medical data covering the six months prior to staphylococcal isolation were analysed by multivariable logistic regression. The identity of all MRSP isolates was confirmed through demonstration of S. intermedius-group specific nuc and mecA. In the final model, cats (compared to dogs, OR 18.5, 95% CI 1.8–188.0, P = 0.01), animals that had been hospitalised (OR 104.4, 95% CI 21.3–511.6, P < 0.001), or visited veterinary clinics more frequently (>10 visits OR 7.3, 95% CI 1.0–52.6, P = 0.049) and those that had received topical ear medication (OR 5.1, 95% CI 1.8–14.9, P = 0.003) or glucocorticoids (OR 22.5, 95% CI 7.0–72.6, P < 0.001) were at higher risk of MRSP infection, whereas S. pseudintermedius isolates from ears were more likely to belong to the MSSP-group (OR 0.09, 95% CI 0.03–0.34, P < 0.001). These results indicate an association of MRSP infection with veterinary clinic/hospital settings and possibly with chronic skin disease. There was an unexpected lack of association between MRSP and antimicrobial therapy; this requires further investigation but may indicate that MRSP is well adapted to canine skin with little need for selective pressure.  相似文献   

Impact of novel endophytes in perennial ryegrass on herbage production and insect pests from pastures under dairy cow grazing in northern New Zealand     

E. R. Thom  A. J. Popay  C. D. Waugh  E. M. K. Minneé 《Grass and Forage Science》2014,69(1):191-204

Perennial ryegrass (Lolium perenne L.) infected with a novel endophyte (AR37 or AR1), Wild‐type endophyte or no endophyte (Nil) was sown with white clover (Trifolium repens L.) in autumn 2005. The pastures were rotationally grazed by dairy cows from 2005–2009. Annual dry matter (DM) yield did not differ but AR37 pastures had a higher ryegrass tiller density, especially after the 2008 summer drought (+130%), and less white clover than did AR1 pastures. Concentrations of alkaloids produced by the Wild‐type association (lolitrem B, ergovaline) followed the same seasonal trends as did the AR37 alkaloids (epoxy‐janthitrems) but summer drought reduced concentrations of lolitrem B and epoxy‐janthitrems to less than half the mid‐summer (February) peak concentrations in the other years. Insect pests were monitored annually between 2006 and 2009. Tiller damage by Argentine stem weevil (Listronotus bonariensis (Kuschel)) was significantly reduced by all endophyte treatments. African black beetle (Heteronychus arator (F.)) populations in soil samples increased during the experiment with Nil > AR1 > Wild‐type = AR37. Root aphid (Aploneura lentisci (Pass.)) infestations followed the pattern AR1 > Nil > Wild‐type = AR37. A lower pest pressure from all insect pests in AR37 pastures is likely to have contributed to this treatment having the highest ryegrass tiller densities.  相似文献   

Action research on alternative land tenure arrangements in Wenchi,Ghana: learning from ambiguous social dynamics and self-organized institutional innovation     

Samuel Adjei-Nsiah  Cees Leeuwis  Ken E. Giller  Thom W. Kuyper 《Agriculture and Human Values》2008,25(3):389-403

This study reports on action research efforts that were aimed at developing institutional arrangements beneficial for soil fertility improvement. Three stages of action research are described and analyzed. We initially began by bringing stakeholders together in a platform to engage in a collaborative design of new arrangements. However, this effort was stymied mainly because conditions conducive for learning and negotiation were lacking. We then proceeded to support experimentation with alternative arrangements initiated by individual landowners and migrant farmers. The implementation of these arrangements too ran into difficulties due to intra-family dynamics and ambiguities regarding land tenure. Further investigations to find out how ambiguities could be tackled revealed that the local actors themselves had taken initiatives towards developing institutional innovations to reduce ambiguities. However, there is still considerable scope for further development of these self-organized innovations. The article ends with a reflection on inter-disciplinary action research, where it is argued that making “mistakes” is an inherent and necessary characteristic in action research that aims to address complex social issues.

Samuel Adjei-NsiahEmail:

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Hydroxylysyl pyridinoline cross-link concentration affects the textural properties of fresh and smoked Atlantic salmon (Salmo salar L.) flesh     

Li X  Bickerdike R  Lindsay E  Campbell P  Nickell D  Dingwall A  Johnston IA 《Journal of agricultural and food chemistry》2005,53(17):6844-6850

A simple HPLC method is presented to quantify the low concentration of hydroxylysyl pyridinoline (PYD) cross-links in Atlantic salmon (Salmo salar L.) muscle. The method involved the extraction of tissue with NaOH prior to hydrolysis, which greatly reduced the amount of protein to be hydrolyzed and made downstream operations easier and more reproducible. The concentration of PYD was 426 pmol g(-)(1) dry mass muscle in post-rigor muscle stored at 0 degrees C and sampled 3 d after death. Hydroxproline (HYP) concentration was determined following NaOH extraction as a measure of collagen content. In post-rigor samples, the alkaline-insoluble HYP fraction comprised 18.3% of the total HYP. Scanning electron microscopy revealed shrinkage of muscle fibers and a retraction of the connective tissue matrix in smoked salmon. PYD concentration was relatively resistant to processing to the smoked product, decreasing by around 11.7%, as compared to a 22.2% decrease in HYP. There was a positive correlation between PYD concentration and the firmness of post-rigor muscle samples as measured by an instrumental texture analyzer, explaining 25% of the total variation. A weaker but still significant correlation was found between PYD concentration and firmness in the smoked product. There was no relationship between fillet firmness and total collagen concentration, although the correlation with HYP in the alkaline-insoluble fraction was significant at the 6% level (P = 0.057). Our results indicate that only 1-3% of collagen molecules are linked by nonreducible mature cross-links in harvest size farmed Atlantic salmon and that PYD concentration is an important raw material characteristic for flesh quality.  相似文献   

Functional morphology and microarchitecture of the circumcaudal organ of Cervus elaphus and C. nippon     

R R Hofmann  H Thomé 《Berliner und Münchener tier?rztliche Wochenschrift》1986,99(12):418-424

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Crop Improvement through Plant Cell and Tissue Culture     

Nickell LG  Torrey JG 《Science (New York, N.Y.)》1969,166(3908):1068-1070

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Solvent-mediated disruption of bovine casein micelles at alkaline pH     

Vaia B  Smiddy MA  Kelly AL  Huppertz T 《Journal of agricultural and food chemistry》2006,54(21):8288-8293

The disruption of casein micelles at alkaline pH was investigated using turbidity measurements. The rate and extent of disruption of casein micelles at alkaline pH (8.0-11.0) increased with pH. Furthermore, the extent of alkaline disruption increased with increasing temperature (5-40 degrees C). Preheating milk for 10 min at 90 degrees C did not influence the extent of alkaline disruption of casein micelles, suggesting that whey proteins do not influence the alkaline disruption process. Levels of ionic calcium and serum calcium and phosphate decreased in a logarithmic fashion with increasing pH, indicating precipitation of calcium phosphate onto the casein micelles. A mechanism for alkaline disruption of casein micelles is proposed, in which increasing the milk pH improves the solvent quality for the caseins, thereby leading to the disruption of casein micelles into their constituent nanoclusters; increases in the net-negative charge on the caseins on increasing pH may contribute to micellar dissociation.  相似文献   

The effect of tuberculin testing on the development of cell-mediated immune responses during Mycobacterium bovis infection     

Thom ML  Hope JC  McAulay M  Villarreal-Ramos B  Coffey TJ  Stephens S  Vordermeier HM  Howard CJ 《Veterinary immunology and immunopathology》2006,114(1-2):25-36

Protection against tuberculosis (TB) is associated with Th1-type cell-mediated immunity (CMI). Whilst the intradermal injection of partially purified derivatives of tuberculin (PPD) represents the classic test assessing the delayed type hypersensitivity (DTH) response used in both humans and cattle for diagnosing TB, it has been suggested that the test may modulate host CMI responses. To investigate the kinetics of the development of the DTH response and its subsequent effect on CMI responses, groups of 6-month old calves were inoculated intranasally with 8 x 10(4) cfu of Mycobacterium bovis, subjected to the comparative intradermal tuberculin test (TT) using bovine and avian PPD (PPD-B, PPD-A) at various time intervals post-infection, and immune responses compared. These included DTH, lymphocyte proliferation, IgG production, and synthesis of the cytokines: IFNgamma, IL-10, IL-4, IL-6, and IL-13. All animals were subjected to post-mortem examination. The kinetics of the development of the DTH response assessed in the TT was such that infected cattle could be identified as early as 3 weeks post-infection, which correlated with the detection of an antigen-specific IFNgamma response. Transient increases in plasma-derived IFNgamma as a result of TT during an established TB infection were more pronounced when blood was stimulated with PPD-A compared with PPD-B stimulation. This has the potential to mask diagnosis of infection as a result of the stronger avian-bias if the IFNgamma test is used the week following TT. Disease pathology was not affected by TT. A transient failure to a second TT was observed in 1 of 30 animals and the time (post-infection) at which the TT is administered may be of significance. In serum, IgG responses to PPD-B, which were undetectable prior to TT, were elevated after TT and were most pronounced in cattle that were TT at 6 weeks post-infection. Other cytokines were also affected by the TT; IL-4 mRNA levels increased and IL-6 mRNA levels decreased, whilst PPD-B specific IL-10 protein synthesis was enhanced. These observations may offer the potential for further diagnostic assays that could complement the TT and IFNgamma test.  相似文献