Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to glycoprotein antigen of bovine herpesvirus type 1     

N Nakajima  E Oishi  T Okabe  I Iwamoto  N Sasaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(5):1063-1066

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Clinical pharmacokinetics of oseltamivir and its active metabolite oseltamivir carboxylate after oral administration in horses     

Yamanaka T  Yamada M  Tsujimura K  Kondo T  Nagata S  Hobo S  Kurosawa M  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):293-296

The aim of this study was to investigate the pharmacokinetics of oseltamivir carboxylate (OC) in horses (n=6) after oral administration of its prodrug oseltamivir. The binding rate of OC to horse plasma proteins was negligible (<1%). Oral administration of oseltamivir of 2 mg/kg body weight of oseltamivir to horses provided a plasma concentration of OC (mean maximum concentration: 257.9 ng/ml) above the inhibitory concentrations against equine influenza A viruses determined in vitro. However, because OC is rapidly eliminated from horse plasma (mean elimination half-life: 2.5 hr), administration intervals should be less than 10 hr to retain a suitable concentration when using a single dose of 2 mg/kg oseltamivir.  相似文献   

Molecular characterization of the equine herpesvirus 1 strains RacL11 and Kentucky D     

Ghanem YM  Ibrahim el-SM  Yamada S  Matsumura T  Osterrieder N  Yamaguchi T  Fukushi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):573-576

The pathogenicities of RacL11 and Kentucky D strains of equine herpesvirus 1 in the hamster infection model are different from those of Ab4p and the Japanese isolates. Virus genome restriction fragment length polymorphism analysis and sequence comparison of an intergenic region, glycoproteins and tegument genes showed higher conservation but with some strain-specific differences. These results indicate that point nucleotide differences in RacL11 and Kentucky D might be responsible for their pathogenicity in rodent models.  相似文献   

Occurrence of chrysanthemum virescence caused by “<Emphasis Type="Italic">Candidatus</Emphasis> Phytoplasma aurantifolia” in Okinawa     

Takashi Naito  Minoru Tanaka  Satoshi Taba  Tesuya Toyosato  Atsushi Oshiro  Kazuko Takaesu  Kazuo Hokama  Tomio Usugi  Shinji Kawano 《Journal of General Plant Pathology》2007,73(2):139-141

In 1999, a disease of chrysanthemum [Dendranthema grandiflorum (Ramat.) Kitamura], characterized by virescence of flowers, occurred in Okinawa Prefecture. The causal agent was identified as “Candidatus Phytoplasma aurantifolia” based on 16S rDNA sequencing. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB247462.  相似文献   

Protective immunity of sevenband grouper, Epinephelus septemfasciatus Thunberg, against experimental viral nervous necrosis   总被引：9，自引：0，他引：9  

S Tanaka  K Mori  M Arimoto  T Iwamoto  & T Nakai 《Journal of fish diseases》2001,24(1):15-22

This paper describes the protective immune responses of sevenband grouper, Epinephelus septemfasciatus Thunberg, immunized with live piscine nodavirus, the causative agent of viral nervous necrosis (VNN), or the Escherichia coli – expressed recombinant coat protein. Nodavirus-neutralizing antibodies were detected at titres ranging from 1:158 to 1:1257 in serum of sevenband grouper which survived intramuscular injection with the virus, by a cell culture assay system. The virus-neutralizing ability of immune serum was also confirmed by injecting virus previously treated with serum into fish. This indicates establishment of acquired immunity in survivors and thus explains why survivors from natural infection are resistant to recurrence of the disease. Young sevenband grouper were immunized twice by intramuscular injections with the recombinant coat protein. Immunized fish produced neutralizing antibodies at high titres for at least 110 days and showed significantly lower mortalities in virus challenge tests. These results suggest the potential for vaccination against VNN in sevenband grouper, which is susceptible to piscine nodavirus at all life-stages.  相似文献   

Transfection of striped jack nervous necrosis virus (SJNNV) RNA into fish cells   总被引：1，自引：0，他引：1  

Iwamoto  Nakai  Mori  Arimoto  Mise  & Furusawa 《Journal of fish diseases》2001,24(3):185-188

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Nodavirus infection of juvenile white seabass, Atractoscion nobilis, cultured in southern California: first record of viral nervous necrosis (VNN) in North America     

P A Curtis  M Drawbridge  T Iwamoto  T Nakai  R P Hedrick  & A P Gendron 《Journal of fish diseases》2001,24(5):263-271

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Evidence for brainstem structures participating in oculomotor integration     

Nakamagoe K  Iwamoto Y  Yoshida K 《Science (New York, N.Y.)》2000,288(5467):857-859

The cerebellar flocculus has been implicated in vestibulo-oculomotor control. One major central input to this structure originates from brainstem cells in the paramedian tract (PMT), whose function is unknown. Here it is reported that PMT cells in the pons carry vestibular and eye movement signals and their pharmacological inactivation produces a leaky integrator combined with vestibular imbalance. The results suggest that PMT cells provide the cerebellum with sensory and motor signals that are essential for velocity-to-position integration, a common premotor process that is required in all motor systems.  相似文献   

Distribution of <Emphasis Type="Italic">tomato chlorotic dwarf viroid</Emphasis> in floral organs of tomato     

Yosuke Matsushita  Tomio Usugi  Shinya Tsuda 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(4):441-447

In situ hybridization was used to analyze the distribution pattern of Tomato chlorotic dwarf viroid (TCDVd) in floral organs of tomato plants. Following TCDVd invasion of floral organs, it became localized only in sepals at an early developmental stage, then reached other floral organs at the flower opening stage, with the exception of part of the placenta and ovules. When distribution of TCDVd was compared with that of Potato spindle tuber viroid (PSTVd), TCDVd was not detected in the outer integument around the embryo sac even though PSTVd was able to invade there, suggesting that such specific distribution might reflect the frequent occurrence of viroid disease on crops caused by PSTVd-seed transmission.  相似文献   

Genomic diversity among equine herpesvirus-4 field isolates     

Maeda K  Kai K  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(6):555-561

Infection with equine herpesvirus-4 (EHV-4) is a major cause of respiratory tract disease, equine rhinopneumonitis, in horses. Although the full sequence of EHV-4 has been reported, genomic differences among EHV-4 field isolates have not yet been characterized. In this study, the genomic diversity between 23 Japanese EHV-4 isolates was analyzed by digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR). The restriction endonuclease digestion patterns of the EHV-4 field isolates showed distinct differences which included mobility shifts of some fragments as well as loss and/or gain of fragments. Two EHV-4 genes containing repeat sequences, ORFs 24 and 71, were amplified by PCR and the amplified fragments were compared among the field isolates. The sizes of the amplified fragments varied among epizootiologically unrelated isolates, while the fragments of related isolates had the same size. The observed genomic diversity among EHV-4 field isolates may be a useful tool for epidemiological study of equine rhinopneumonitis by EHV-4 infection.  相似文献